VPS10_ASHGO
ID VPS10_ASHGO Reviewed; 1508 AA.
AC Q754Q4;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; OrderedLocusNames=AFR018C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; AE016819; AAS53389.2; -; Genomic_DNA.
DR RefSeq; NP_985565.2; NM_210919.2.
DR AlphaFoldDB; Q754Q4; -.
DR SMR; Q754Q4; -.
DR STRING; 33169.AAS53389; -.
DR EnsemblFungi; AAS53389; AAS53389; AGOS_AFR018C.
DR GeneID; 4621804; -.
DR KEGG; ago:AGOS_AFR018C; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_1_1; -.
DR InParanoid; Q754Q4; -.
DR OMA; IFMHVTT; -.
DR Proteomes; UP000000591; Chromosome VI.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..1508
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407502"
FT TOPO_DOM 21..1348
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1349..1369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1370..1404
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1405..1425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1426..1508
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 164..172
FT /note="BNR 1"
FT REPEAT 372..381
FT /note="BNR 2"
FT REPEAT 452..461
FT /note="BNR 3"
FT REPEAT 497..507
FT /note="BNR 4"
FT REPEAT 717..726
FT /note="BNR 5"
FT REPEAT 812..822
FT /note="BNR 6"
FT REPEAT 1095..1105
FT /note="BNR 7"
FT REPEAT 1137..1146
FT /note="BNR 8"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 789
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 911
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1243
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1346
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1508 AA; 169235 MW; 82214594B3CC3129 CRC64;
MVGMLFVWQT FLFVWACAAA VEVGEKPRPV IDIVRDVDNL GSIWQFKGTN TLIRHQGREI
LVRHGGDQWS TVKKFDQGVN TVEVDPYYPE RRAFAWLENN ELYKTDDCGK TWARVMHVEA
EPGENLQRYS VYSNPDNYKY LMVTSHFERK DSSPVLAESY EKQWVSQDGG KTIAPLGHPD
PGVYRLCHFL RQARNHDVAK DSTILCIEHD AQKHTAVLYR TDDFGKSKQA LDSFDDEIVS
SVGLLGKYIL VTTTEDAYNK HAVQHVWVSR DGDKFQKIIF PTHIRDNFFR AVTTSTGNQI
IFSIPAGKHS HSRKNALYAN YISDSSGIQF HAVADSLDER YKELGPVMAL DANGTLSMVA
RKSSTEDQVT LLSYDYGYSW QPMHLAEGED HKALGCSKGG KECELPVQLS QLVSSSGLVG
HGESATSGIL MGIVLKKRGS RGPRKHKYLS VISRDYGLTW SVAFEYPVLS FAGNYGNILL
ACPYNPGDDG DPEEEIYYSL DQGHTWEEHH IEGKYEFVRV HSAISDGSST AFSFLAVGDD
QNTIVVDCIF TDIHNGKECT DGDMEEYKLR NGNCLNGARY TINKRKSEAA CLLKNDPSVI
NPLVKPCDCT NADYECSLGF LGLPDKGCTA DLGMLRSAGA CKDNPRELMP MHKIKGNECT
KDLNIEPVKV DCSAVQNRPD IEVTAHVFKT QFETYQYFNS AEDDSLMVLT KDKRALISHD
SGKTFKMLDT LGSGAELITF NRYFGDLAYI FASDDVLYIT DDRGYHFYAV DLPEVRHLFL
PLAFHAKDNQ TFIYFGGKDC EKLGPKCHST AYITTDGGRN FREMLTGAVS CEFVGSIYSH
PANKDMIMCE IENKKERRNI LLRSTDEFRT SDKVFDSIIG FHTTGDFTAI AVSEGRQQVS
SYLTIDGVHF NESRFPPDFV APEKQQSYSV LSAHEGAIFL HMSKSLVKHK EYGTLVKSDS
NGIDFVVLKD GVNSNAEGLV DFETPEGLDG VILINVVENL DQVQKGKAKA KHLKTAISFN
DGVDWKYLSP PGKDSSGKKY PCHGKNRAKC SLNLHGYTER KDLRDTYSSG SAIGYMLGVG
NVGEHLLPYE QCSTFLTTDG GVTWTEVKST PHQWEFGDRG SIIVLVPDGV KTNSITYSVD
AGRSWQDFKF ADEEVIIDDI ITVPYDTSMR FLLISKEHLR GKSKTYAISF AHIFKRQCEF
HPGGGRHNGD FEYTSIKHPD YKCLFGKEVE FLKRIKYDCF IGNASYTKDY RTSRICPCTR
QDFECDYNYI RTSDGTCKLP SGVKPEAPSA VCERNRDLVE YFQPTGYRKI PLSKCEGGLK
LQRTDSPHPC PGKEKEFYEK YPSSTNASSV VFWWLLLTMV LLVPLWVIYD RGIRRNGGFS
RFGEIRLDDD DLIEENGLDR AINKVVKVGA YGVAGLFGFL LLLKSKAGAR IRRFRESVSS
RRGPSYSSLI SDQFLDDAND LLVGHDNDAN NLNAFLDEDG QHFDVDDEIE GADQASYHDD
VDLGRTSE
