VPS10_BLAGS
ID VPS10_BLAGS Reviewed; 1500 AA.
AC C5JC96; A0A179U5W1;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; ORFNames=BDBG_00123;
OS Blastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Blastomyces.
OX NCBI_TaxID=559298;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SLH14081;
RX PubMed=26439490; DOI=10.1371/journal.pgen.1005493;
RA Munoz J.F., Gauthier G.M., Desjardins C.A., Gallo J.E., Holder J.,
RA Sullivan T.D., Marty A.J., Carmen J.C., Chen Z., Ding L., Gujja S.,
RA Magrini V., Misas E., Mitreva M., Priest M., Saif S., Whiston E.A.,
RA Young S., Zeng Q., Goldman W.E., Mardis E.R., Taylor J.W., McEwen J.G.,
RA Clay O.K., Klein B.S., Cuomo C.A.;
RT "The dynamic genome and transcriptome of the human fungal pathogen
RT Blastomyces and close relative Emmonsia.";
RL PLoS Genet. 11:E1005493-E1005493(2015).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; GG657448; OAT03396.1; -; Genomic_DNA.
DR RefSeq; XP_002628877.1; XM_002628831.1.
DR AlphaFoldDB; C5JC96; -.
DR SMR; C5JC96; -.
DR STRING; 559298.C5JC96; -.
DR EnsemblFungi; OAT03396; OAT03396; BDBG_00123.
DR GeneID; 8507980; -.
DR KEGG; bgh:BDBG_00123; -.
DR VEuPathDB; FungiDB:BDBG_00123; -.
DR HOGENOM; CLU_000700_0_0_1; -.
DR Proteomes; UP000002038; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR036278; Sialidase_sf.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
DR SUPFAM; SSF50939; SSF50939; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..1500
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407498"
FT TOPO_DOM 23..1364
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1365..1385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1386..1412
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1413..1433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1434..1500
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 378..387
FT /note="BNR 1"
FT REPEAT 438..449
FT /note="BNR 2"
FT REPEAT 480..490
FT /note="BNR 3"
FT REPEAT 724..734
FT /note="BNR 4"
FT REPEAT 821..831
FT /note="BNR 5"
FT REPEAT 1108..1118
FT /note="BNR 6"
FT REPEAT 1150..1159
FT /note="BNR 7"
FT REGION 1481..1500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 298
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 322
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 810
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 973
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1500 AA; 168145 MW; 684C43A160883560 CRC64;
MILRRLLLAA GSLLLASAFT SAKKADGPKI TVTKFKHEPV NLFYFDDSDV VMLQDGNNGD
VYVSRNAGTK WDIVDVGEMK GQAWSLMPHP MDRTKAYVLG KGGKHWVTND QAMTWREFTV
DAELSPYQLP LVFHGKDSNR VMLQGIKCSG RDCVERTYYT TDGFQTLHLL MEKGRHCAWA
VSTPMFGDGL DLPKEVNDRI FCVVSGLHSQ WPEDNRLLYS DRFFKDDSGI EAGLDSGRAV
SGVIRTASAK RHLLAATKSR RTNELALFVT DDASGWHRAE FDGQRIEEDA YTILESTNYS
LQVDVVSTDS SAMGTLFTSN SNGTYFTRNI EHTNRNRQGL VDFEKIATIQ GIILVNTVKN
WEDVEKSSVV EKKIVSRISF DDGRTFQPLK AGKQDLHLHS VTDATNYGRV FSSPAPGLVM
GVGNTGGHLK DYSDGDLYIS DDAGINWKKA LSDAHKYEFG DQGSVIVAVY DEGRTNKISY
SFNHGKSWEK ASLPLPDDVK IRAKILTTMP DSTSLKFVLV GSAKADSDVE HYVIAIDFAE
MEERTCVDPD FEKWPARLNE KQEPDCLMGH KQFYRRRKAD ANCFIKKKFE DPIPNFEPCK
CTEEDFECDF NFIRSEDGKS CVPARSLLAP EGACKNPDDK YTGSSGFRLI PGDACIKKGG
LELDKPIERV CSDTMKTPAA GEIAVEKTFF TADNYRDYFY LERTGSSRGD DETVIMITSE
QEIFISRDHG KRWKRIFEAE TITRIEPHRY FNDVAYFLTN SGEGWYTLDR GENFRKFKAP
LPPNQDKLPA LSFHPDRRDW LIWTGAEECN GSGGQCHSVA YYTTNRGAEW HFLMRYVRRC
EFIKRDARGS SDKIVFCEQY EDENPSSKHL QLISSEDWFA EKKVHYNNIL DFATMQEFII
VAIRGEKPQD SLSVGVSIDG KTFADAELPA NVKIPIQRAY TVLESRTHAA FLHVTVNNLE
DHEYGSLIKS NSNGTSYVLS LSAVNRNSRG YADFEKMQGL EGVAMANVVA NVDDVEKGAA
KKYRTMITHN DGAEWTLLAP PGKDSEGRAY GCSTKGGKPT KACALHLHSY TERVDPRDTY
SSPGAVGIMI GTGNVGEYLT PKSSADTFIT RDAGISWEEA KKGKYQWEYG DSGSIIVLVP
ESTPTKTLLY SLDEGRSWKE YQFSEVEMQI RDISTVPSDT SRNFLLWGNE VGQGKKPGLA
TVNIDFSGLK ERSKQCVLNE EKPEADDYYL WEPKHPLQPN GCLFGHRAKY HRKRPEKDCY
NGREIQHLDS IGDICECTRS DYECDYNYEP QTDGTCARVP GLEPLDPKLV CTEDPKAIEW
YEPTGYRRIP LTKCQGGKQL NHIIAHPCPN KEEEFEKKHP RLRGIGLFFA IVIPIGLAAA
VGYYVYQHWD GKFGRIRLGE TGSGGFFDRD SPLISIPVTI VAGIVAVATA LPLLVSSLWR
SLGGYIRVPG SGSSRQPYSS RGAFAARRGD YVGVVDDEDE LLGTDDLEDD EEGEERNGQV
