VPS10_EMENI
ID VPS10_EMENI Reviewed; 1416 AA.
AC Q5AS50; C8VLU2;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin vps10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor vps10;
DE Flags: Precursor;
GN Name=vps10; Synonyms=vpsT; ORFNames=AN8880;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=21226815; DOI=10.1111/j.1600-0854.2011.01164.x;
RA Pantazopoulou A., Penalva M.A.;
RT "Characterization of Aspergillus nidulans RabC(Rab6).";
RL Traffic 12:386-406(2011).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000269|PubMed:21226815}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21226815}. Prevacuolar compartment membrane
CC {ECO:0000269|PubMed:21226815}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21226815}. Note=Cycles between the Golgi apparatus
CC and the prevacuolar compartment.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAA64094.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AACD01000164; EAA64094.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BN001307; CBF84749.1; -; Genomic_DNA.
DR RefSeq; XP_682149.1; XM_677057.1.
DR AlphaFoldDB; Q5AS50; -.
DR SMR; Q5AS50; -.
DR STRING; 162425.CADANIAP00007992; -.
DR PRIDE; Q5AS50; -.
DR EnsemblFungi; EAA64094; EAA64094; AN8880.2.
DR GeneID; 2868185; -.
DR KEGG; ani:AN8880.2; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; Q5AS50; -.
DR OrthoDB; 1046610at2759; -.
DR Proteomes; UP000000560; Chromosome VII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR036278; Sialidase_sf.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 3.
DR SMART; SM00602; VPS10; 2.
DR SUPFAM; SSF50939; SSF50939; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..1416
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407518"
FT TOPO_DOM 24..1284
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1285..1305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1306..1333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1334..1354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1355..1416
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 62..71
FT /note="BNR 1"
FT REPEAT 374..384
FT /note="BNR 2"
FT REPEAT 436..446
FT /note="BNR 3"
FT REPEAT 478..487
FT /note="BNR 4"
FT REPEAT 714..724
FT /note="BNR 5"
FT REPEAT 808..818
FT /note="BNR 6"
FT REPEAT 1077..1083
FT /note="BNR 7"
FT REPEAT 1115..1124
FT /note="BNR 8"
FT CARBOHYD 583
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 938
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1416 AA; 158405 MW; 6F33D0B6F995C74C CRC64;
MIPRWIQLVG CLLLALMLPP SAAKKDKPGI SFHKLHEKPY SMFYFEKSDT VLLNLEDGEV
LRSFDAGENW EVIEDNGMKH GVTSIHQHPF DEDKAYALGD DGKHWITTDK AKTWKSFKVP
DQAYFKSPQT GPLTFHGRDS SKVIFETRPC PSCAPRSYYT TDDFDTIEVL TESAVGCYWA
VGNPQFAAGP NMPKGMEERT VCVVPGLKAS SRFAFRLVYS DDYFRSQGIE TKLQDGRPVS
GVTSIAQVKS FIVAAVESQG TTERALYVTT DIEKWHRAEF GDHRLEQDAY TVLESSDYSL
QVDVLTNPFS GMGVLFTSNS DGTYFTRNIE HTNRGPRGYV DFEKLAAIQG IVLVNTVRNP
DDVEAGASKE VVSQISFDDG RTFQPLKSTD GQRIHLHSVN SDTNLGRVFS SPAPGLVMGV
GNTGDYLGKR SDGHLYVSDD AGLTWRLALK GPHKYEFGDQ GAVLVAISEA PKVKKIEYSL
DHGKEWKSVD LPHEVDGETS TLTTTPDSTS LKFILLGHSK GEPSVYAIDF EDLHERKCED
EDFDNHWPAR LDEHGEPDCL MGQKQFFRRR KANADCFVAE AFNSSMSKFE PCKCTVEDFE
CQSTRTEDGK DCVPPKSFTP PDGDCINPDD TTMIPSGWRL IPGDVCVRDD GVNLDKDVEI
PCKDVNNRPK SKEITSTMKM FSSGLSAYRY LERQVSNLGE DETVLMLSRN QELFVSHDHG
QTWQQELKGA SIMKIVPHPY NSDTAYILTD SEEVFYTINR GATFGSFRAK TLPDYENGPI
LRFHPLKRDW LLWVGTECDS GSCHSNAYFS DDRGDSWKTI LRHAKKCEFE YKENRPDSLY
LVFCEQYENE DDKKRLQLMS TTDERFSDWE TVEENLVEYA TRAEYIILAS HTDKDNALKA
RVSVDGTTFA DLKFPPNVVP AQNLYTLVDA SEHAIFLNGT TYVLSLDAVN QNDWGYVDFE
RMQALEGVII ANIVSNVDEL SDGAPKRLRT MITHNDGGEW TLLAPPTKDA NGKKFPCSVV
EGKGTEDCAL HLHGYTERAD PRDTFSSGSA IGLMMGLGNV GDRLTSKDEA DTFLTMDGGI
TWKSVKKGRY MWEYGDSGSV IVIVSEQKST KVLHYSTDEG ATWQDYYFSD EEIEVIDIST
VPSDTSKKFI LWGKKSDELV TVNVDFSGLY DRDCEFDDKG GDVSDDYQLW TPKHPFQEEN
SDYECDYNYE RQNDGTCKLV PGLKPHDPVG YCKAHPDAIE YFEPTGYRRI PQTTCQGGYN
LDHQTAKPCP GKQQEYDKKH GISGIGLFFA IVAPIAVAGA VGYYIYTKWD GKFGQIRLGE
TSTGTQGILS RDSLLVTVPI AIIAGIVAVA KALPLLVTSL WRSASGYMRV GNRSYPRPYA
SRASFAARRG DYSSVVEDED ELLGVVDFDG DEEEDS
