VPS10_KOMPG
ID VPS10_KOMPG Reviewed; 1542 AA.
AC C4R192;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; OrderedLocusNames=PAS_chr2-1_0625;
OS Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=644223;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS115 / ATCC 20864;
RX PubMed=19465926; DOI=10.1038/nbt.1544;
RA De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT "Genome sequence of the recombinant protein production host Pichia
RT pastoris.";
RL Nat. Biotechnol. 27:561-566(2009).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; FN392320; CAY69266.1; -; Genomic_DNA.
DR RefSeq; XP_002491546.1; XM_002491501.1.
DR AlphaFoldDB; C4R192; -.
DR SMR; C4R192; -.
DR STRING; 644223.C4R192; -.
DR EnsemblFungi; CAY69266; CAY69266; PAS_chr2-1_0625.
DR GeneID; 8198939; -.
DR KEGG; ppa:PAS_chr2-1_0625; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; C4R192; -.
DR OMA; CEISAYI; -.
DR Proteomes; UP000000314; Chromosome 2.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR036278; Sialidase_sf.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
DR SUPFAM; SSF50939; SSF50939; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..1542
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407532"
FT TOPO_DOM 21..1387
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1388..1408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1409..1445
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1446..1466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1467..1542
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 56..65
FT /note="BNR 1"
FT REPEAT 102..112
FT /note="BNR 2"
FT REPEAT 373..383
FT /note="BNR 3"
FT REPEAT 439..449
FT /note="BNR 4"
FT REPEAT 484..494
FT /note="BNR 5"
FT REPEAT 732..742
FT /note="BNR 6"
FT REPEAT 1128..1138
FT /note="BNR 7"
FT REPEAT 1170..1178
FT /note="BNR 8"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 633
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 714
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 922
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 989
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1542 AA; 173835 MW; EFA9944A7A879667 CRC64;
MWIERNLIAS ILLFSTSAYA AFKPRIVKKE FDDLLNPIYF NDSSTVLGLV DQTLLISNDD
GKSWTNLQEV ITPGEIDPLT IVNIEFNPSA SKAFVFTASK HYLTLDKGST WKEFQIPLEK
YGNRIAYDVE FNFVNEEHAI IRTRSCKRRF DCKDEYFYSL DDLQSVDKIT ISDEIVNCQF
SQSSTSSDSR KNDAITCVTR KLDSNRHFLE SNVLTTLNFF KDVTSLPASD PLTKMLIKDI
RVVQNYIVLF VSSDRYNKYS PTLLFISKDG NTFKEASLPD SEGTSPSVHF LKSPNPNLIR
AIRLGKKNSL DGGGFYSEVL QSDSTGLHFH VLLDHLEANL LSYYQIENLA NLEGIWIANQ
IDTSSKFGSK SVITFDAGLT WSPVTVDEDE DKSLHIIAFA GENSLYESKF PVSTPGIALR
IGLIGDSSDA LDIGSYRTFL TRDAGLTWSQ VFDNVSVCGF GNYGNIILCC SYDPLLRSEP
LKFRYSLDQG LNWESIDLGF NGVAVGVLNN IDNSSPQFLV MTIATDGKSS KAQHFLYSVD
FSDAYEKKIC DVTKDELFEE WTGRIDPVTK LPICVNGHKE KFRRRKADAE CFSGELFQDL
TPIEEPCDCD PDIDYECSLG FEFDAESNRC EPNLSILSSH YCVGKNLKRK VKVDRKSKVA
GTKCKKDVKL KDNSFTLDCS KTSEPDLSEQ RIVSTTISFE GSPVQYIYLK QGTNTTLLDE
TVILRTSLRT VYVSHNGGTT FDRVSIEDDV SFIDIYTNHY FPDNVYLITD TDELYVSDNR
AISFQKVDMP SRAGLELGVR ALTFHKSDPN KFIWFGEKDC NSIFDRSCQT QAYITEDNGL
SFKPLLENVR SCYFVGTTFD SKLYDFDPNL IFCEQRVPNQ RFLKLVASKD YFYDDKEELY
PKIIGIATTM SFVIVATINE DNRSLKAFIT ADGSTFAEQL FPADLDFGRE VAYTVIDNWE
SKTPNFFFHL TTSEDKDLEF GALLKSNYNG TTYTLAANNV NRNDRGYVDY EIVLNLNGIA
LINTVINSKE LESEQSLETA KKLKTQITYN DGSEWVYLKP PTIDSEKNKF SCVKDKLSLE
KCSLNLKGAT DRPDSRDSIS SGSAVGLLFG VGNVGEYLNQ DSSGLALYFS KDAGISWKEI
AKGDYMWEFG DQGTILVIVE FKKKVDTLKY SLDEGETWFD YKFANEKTYV LDLATVPSDT
SRKFIILANR GEEGDHETVV HTIDFSKVHQ RQCLLNLQDS NAGDDFEYWS PKNPSAVDGC
MLGHEESYLK RIASHSDCFI GNAPLSEKYK VIKNCACTRR DYECDYNFAL ANDGTCKLVE
GESPLDYSEV CRRDPTSIEY FLPTGYRKVG LSTCEGGLEL DNWNPVPCPG KTREFNRKYG
TGATGYKIVV IVAVPLLVLL SATWFLYEKG IKRNGGFARF GVIRLGEDDD DDLQMIEENN
TDKVVNVVVK GLIHAFRAVF VSYLFFRKRA AKMFGGSSFS HRHILPQDED AQAFLASDLE
SESGELFRYA SDDDDAREID SVIEGGIDVE DDDEENINFD SR
