VPS10_LACBS
ID VPS10_LACBS Reviewed; 1469 AA.
AC B0E2U2;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; ORFNames=LACBIDRAFT_192355;
OS Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver)
OS (Laccaria laccata var. bicolor).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Tricholomataceae; Laccaria.
OX NCBI_TaxID=486041;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 / ATCC MYA-4686;
RX PubMed=18322534; DOI=10.1038/nature06556;
RA Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F.,
RA Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A., Shapiro H.J.,
RA Wuyts J., Blaudez D., Buee M., Brokstein P., Canbaeck B., Cohen D.,
RA Courty P.E., Coutinho P.M., Delaruelle C., Detter J.C., Deveau A.,
RA DiFazio S., Duplessis S., Fraissinet-Tachet L., Lucic E., Frey-Klett P.,
RA Fourrey C., Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P.,
RA Kilaru S., Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R.,
RA Melayah D., Montanini B., Muratet M., Nehls U., Niculita-Hirzel H.,
RA Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M.,
RA Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U.,
RA Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J.,
RA Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A.,
RA Tuskan G., Grigoriev I.V.;
RT "The genome of Laccaria bicolor provides insights into mycorrhizal
RT symbiosis.";
RL Nature 452:88-92(2008).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; DS547190; EDQ98838.1; -; Genomic_DNA.
DR RefSeq; XP_001890509.1; XM_001890474.1.
DR AlphaFoldDB; B0E2U2; -.
DR SMR; B0E2U2; -.
DR EnsemblFungi; EDQ98838; EDQ98838; LACBIDRAFT_192355.
DR GeneID; 6086167; -.
DR KEGG; lbc:LACBIDRAFT_192355; -.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; B0E2U2; -.
DR OrthoDB; 1046610at2759; -.
DR Proteomes; UP000001194; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..1469
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407520"
FT TOPO_DOM 32..1364
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1365..1385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1386..1412
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1413..1433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1434..1469
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 69..80
FT /note="BNR 1"
FT REPEAT 112..122
FT /note="BNR 2"
FT REPEAT 393..403
FT /note="BNR 3"
FT REPEAT 471..481
FT /note="BNR 4"
FT REPEAT 512..521
FT /note="BNR 5"
FT REPEAT 784..793
FT /note="BNR 6"
FT REPEAT 839..850
FT /note="BNR 7"
FT REPEAT 1049..1058
FT /note="BNR 8"
FT REPEAT 1166..1175
FT /note="BNR 9"
FT CARBOHYD 339
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 995
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1469 AA; 165379 MW; A284FDEFEEF0EAF1 CRC64;
MASTAKARYN VGLKLYTFLL LLLSLIALVL GQEQPVHTIT PFNNLPARLF FFDDTEAAIY
HDSIDGNVYV SQDEGKSWSM AEGIPKGKTV MVIEHPFDNR YAFALTDSKI HYRTEDRGKT
WRSFEVPVPP ALVARPMSFH SDPKKWGSIL YQGTACNRQG WGAICHDETY YTKEAFSDTP
QLLLSETSRC QFAHSSKDFK HEAHSDLIYC VAFDTSSVDG SHALSSSRLF STTDFFDKDR
KVEELGIGKN AKGVIAFAIV SKYAVVALKD LSPSNDGEML LYVTVDTNTW AKAQFPHASS
AKLRENAYTI VESTTHSLAV DVVLQDKSSI GTLFVSNSNG TFFVESLKDT NRNDMGYVDY
EKLYGVDGVG LANVVSNAKD VEGRGARKQL KTMITFDDGT TWTSLRPPSQ DSEGKRISCD
PADTDLCSLH LHSVTTPHNY GRIFSSPAPG FAMGVGSIGE SLLPYDESDT FISTDAGVTW
QMVRRDAHKY EFGDKGSILV VVNDEDGTDN VRYSLDLGKS WLKYDIGIKF RARALMTLPD
STSQRFLLLG QVARKDQTKD TGRVVIIQLD FSGTRKRKCV EGDFEKWYAR TSKTECLMGH
KQWYKRRKPD ADCYVGEKYM DPVEHEDNCQ CTEEDYECDY NFVRNGKDCV PVGPEPIPAG
VCTGNPDQTY KGSSGWRKIP GNTCVDGVKK DEKVDKKCSQ AQPAPGEITH QIHDFKHQIV
QHAYFKNPKV KTILVRLMDH TMWQSSNEGY SWTQIHPEHR FLAFYHHKYA DDRAYLITDT
DTFFYTTDTG RTWIRAKAPT PPNTFGAQVL HFHPNTDNLI WTGNRGCSAQ AQSCHAEAQY
SRDNGRKWSL IDSYVRNCAW AKDAELNTDP NEIICESYRD KKGNQRLFQN ENPMELVTGS
NYYANKRKVF DHVVGFAKFS EFLIVAEMIP ERRSLELEVS LDGIHFATGK FPPSMHPETH
AYTVLESSTK SLFLHMTMSE APAPFWGNIL KSNSNGTYFG LALENVNRDE RGYVDFEKMI
GLDGIALVNV VSNPTDATLS GHKQLQSRIT HNDGSTWRPL TPPVVDSQGN KYSCEGTKCA
LHIHGYTERM DPRATYSSPS IVGVLMAVGN VGETLAPYTE SDTFLSRDAG FTWQEVHKDA
HLWEFGDSGS ILVLANDEEP TDHILFSTDE GLTWREYKFT EEKMRVRSIV TIPSDTSRRF
ILFGSLMRTP GSIAVHIDFT QLTSRKCDLS TDNPAKDDFE MWSPSENRPE QCLFGRQTLY
HRRIRNTDCT VGEQEKAASR VVLNCACSKV DFECEFNHVK NTNDECELVP GTTPLPDSDT
ACRNGEEFWY ERTAYRLIPY SSCTDGERPD RGREHRCPGF KSHSAWFWLF MLLLPFGFTA
LIAFYYYRRS GLARGTIRLP GDGGRPAYGG DTGVVATLAS VPWFIVGVAG IAWEWVVSHV
ERLAPGARSG YRNLPIDEDA QVLRFEDED
