VPS10_NEUCR
ID VPS10_NEUCR Reviewed; 1528 AA.
AC Q7SH60;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin vps10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor vps10;
DE Flags: Precursor;
GN Name=vps10; ORFNames=B16B8.180, NCU02669;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; BX842634; CAE76478.1; -; Genomic_DNA.
DR EMBL; CM002236; EAA36206.1; -; Genomic_DNA.
DR RefSeq; XP_965442.1; XM_960349.3.
DR AlphaFoldDB; Q7SH60; -.
DR SMR; Q7SH60; -.
DR STRING; 5141.EFNCRP00000002058; -.
DR PRIDE; Q7SH60; -.
DR EnsemblFungi; EAA36206; EAA36206; NCU02669.
DR GeneID; 3881583; -.
DR KEGG; ncr:NCU02669; -.
DR VEuPathDB; FungiDB:NCU02669; -.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; Q7SH60; -.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..1528
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407526"
FT TOPO_DOM 25..1385
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1386..1406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1407..1440
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1441..1461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1462..1528
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 62..72
FT /note="BNR 1"
FT REPEAT 105..115
FT /note="BNR 2"
FT REPEAT 450..461
FT /note="BNR 3"
FT REPEAT 494..502
FT /note="BNR 4"
FT REPEAT 749..759
FT /note="BNR 5"
FT REPEAT 1136..1146
FT /note="BNR 6"
FT REPEAT 1179..1189
FT /note="BNR 7"
FT CARBOHYD 308
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 735
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 899
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1006
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1244
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1295
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1528 AA; 172085 MW; BB09FE6CF58ACC5E CRC64;
MRVRGALQAA ALLASALWAA PLLAKDHPTF KTTKLDTQPN NLNYFEGSDT ILFHDTHKSN
LWRSDDAGAT WSVVKDIPDG KVARLYMHDF DSKRAFAITD GRKHYRTTDQ GKTWKTFEVD
AAWDTERFDI LHFHATDPDR IIFNGLHCLG FLCEEVSLYT IDDFETPAKP LRPDTLGCWW
AKSSPVFTTG DSDLDKDRIL CIIMGVDSIF SEDRRLVISD DFFKADKEGN IQEFEPNLAG
DKPVRGVVNV AAVKRYFMVA TTSANTDEMA LFISTDTKKW QRAMFPNAHD DHDHKIVQEA
YTVLESTNYS IQLNVMTGSK SAPMGIMFTS NYDGTYFSEN LEYTNLNMFG HVDFEKIAGI
QGIFLVNKVD NGKEVDEKPS TKKKLVSEIT FDDGRTFDKV TADGKRIHLH SVTELNNVGR
VFSSPAPGLV MANGNTGDYL GDYWEDANLY VSDDAGKTWK KALKGPHKYE FGDQGSILVA
VRDSKEEDIS EISYSLDHGE NWVDEKLPDD LKIQPWILTT TPDSTGLQFV LIGKAKGAWH
VIHIDFEGLH EATCKESDME EWHARVDKDG EPTCIMGHTQ TYPRRKKDAK CFLKKEFKLA
EVETKDCDCT DQDYECDYNF ERNDKGICVS RGPIPIPEGA CKEGDRNGKF LGTSGYRKIP
GNTCKDTKET KDKYKDVERS CGDGIGAPPD EATGELQQVD TKGKFGHWTH WEKHYLERGE
SSSDSSETII MRGMNRTVDD SGPKVGPIWR TTDHGKKWDK VDFFKDDEVI SIVPHPTVKD
WVFFLTEGKK LIYTPDRGKR FRTFEVPQPA DFEAAANGIF PLIFHPDKPN WLIWLGKKCE
SKNDCYRVAY GTKDGERWET LARDVYRCEF TGAEAYGRKY ANRALEQILC LKHEKEGDND
SALQLVSSND WFNQDERVRL KEAKEFATMA EFIVVATEDT EKKTLRAYAS LDGSLYSEAK
FPFGFEVPHQ HAYTVLDSST HAVNLFVATR MEGDKSLGTI LKSNSNGTSY VVSVKNVNCD
QYFYVDFEKM VGLEGVALVN VVVNPDAKSS APKKLQTKIT HNDGAQWAYL PTPRKDEFGK
FPCSSSGTEK CALHIQGYTQ RRDRGKTYSS EGAVGVMFGW GNVGDSLGSI KDADTFMTTD
AGITWKRVKK GRWNWALGDQ GSIIVLVPIR GQKTDTLEYS LDQGATWKKH TFSKEKVDIW
DLTTTRSGNS QNFLIWGENS DGLFTTKIDF TKFTDHVCKY DPENLSKSDY QIFSPKHPLQ
PDGCLFGHVS QYLRKKPGLK CFNDFRLDPL YSKQNCTCTR SDFECDFNYE LDKHGQCSLV
SGLKPKDHKL WCKEHPDEIE YYEPTGFRRI PLTTCQGGLD FEKAAAVHPC PGHEDDFERK
HRVSGIAIFF AVVLPVAAAS AIGWWVYRHW DGKFGQIRLG EQGASTMEFF DADRPWVKYP
VIALSAVVAL AGAMPLVLGA LWRTAKSTAE RWGIGGGGGG RGGWSRLDGG GASRTFRTRD
SFARGRGDYT IVDEDEGELL GEESDEEV
