VPS10_SCHPO
ID VPS10_SCHPO Reviewed; 1466 AA.
AC O42930;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin vps10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor vps10;
DE Flags: Precursor;
GN Name=vps10; Synonyms=pep1; ORFNames=SPBC16C6.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF PHE-1419; PHE-1425 AND
RP PHE-1426.
RX PubMed=16622069; DOI=10.1099/mic.0.28627-0;
RA Iwaki T., Hosomi A., Tokudomi S., Kusunoki Y., Fujita Y., Giga-Hama Y.,
RA Tanaka N., Takegawa K.;
RT "Vacuolar protein sorting receptor in Schizosaccharomyces pombe.";
RL Microbiology 152:1523-1532(2006).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment. {ECO:0000269|PubMed:16622069}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000269|PubMed:16622069}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:16622069}. Prevacuolar compartment membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC Note=Cycles between the Golgi apparatus and the prevacuolar
CC compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA16914.1; -; Genomic_DNA.
DR PIR; T39557; T39557.
DR RefSeq; NP_596804.1; NM_001023825.2.
DR AlphaFoldDB; O42930; -.
DR SMR; O42930; -.
DR BioGRID; 276408; 6.
DR STRING; 4896.SPBC16C6.06.1; -.
DR iPTMnet; O42930; -.
DR MaxQB; O42930; -.
DR PaxDb; O42930; -.
DR PRIDE; O42930; -.
DR EnsemblFungi; SPBC16C6.06.1; SPBC16C6.06.1:pep; SPBC16C6.06.
DR GeneID; 2539861; -.
DR KEGG; spo:SPBC16C6.06; -.
DR PomBase; SPBC16C6.06; vps10.
DR VEuPathDB; FungiDB:SPBC16C6.06; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; O42930; -.
DR OMA; ATMSEFI; -.
DR PhylomeDB; O42930; -.
DR PRO; PR:O42930; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005770; C:late endosome; IDA:PomBase.
DR GO; GO:0005802; C:trans-Golgi network; IDA:PomBase.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0005048; F:signal sequence binding; ISO:PomBase.
DR GO; GO:0006895; P:Golgi to endosome transport; IMP:PomBase.
DR GO; GO:0006896; P:Golgi to vacuole transport; IMP:PomBase.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IMP:PomBase.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Membrane; Nucleotide-binding;
KW Protein transport; Receptor; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..1466
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000316211"
FT TOPO_DOM 34..1363
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1364..1384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1385..1466
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 71..82
FT /note="BNR 1"
FT REPEAT 115..126
FT /note="BNR 2"
FT REPEAT 388..399
FT /note="BNR 3"
FT REPEAT 457..468
FT /note="BNR 4"
FT REPEAT 498..509
FT /note="BNR 5"
FT REPEAT 738..749
FT /note="BNR 6"
FT REPEAT 778..789
FT /note="BNR 7"
FT REPEAT 836..847
FT /note="BNR 8"
FT REPEAT 1039..1050
FT /note="BNR 9"
FT REPEAT 1112..1123
FT /note="BNR 10"
FT REPEAT 1153..1164
FT /note="BNR 11"
FT CARBOHYD 465
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 545
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 986
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 1419
FT /note="F->A: No cpy1 secretion; localizes to Golgi and
FT vacuolar membrane."
FT /evidence="ECO:0000269|PubMed:16622069"
FT MUTAGEN 1425..1426
FT /note="FF->AA: No cpy1 secretion; localizes to Golgi and
FT vacuolar membrane."
FT MUTAGEN 1425
FT /note="F->A: No cpy1 secretion."
FT /evidence="ECO:0000269|PubMed:16622069"
FT MUTAGEN 1426
FT /note="F->A: No cpy1 secretion."
FT /evidence="ECO:0000269|PubMed:16622069"
SQ SEQUENCE 1466 AA; 165062 MW; CEB315E0F7688D79 CRC64;
MFFLTKILPL RGRIFPMFGC LLLIVSLITG CIASPAAEVA ETVFDSKPVD FMTFKDSTNT
LFLNAEFGDV YLSQDNGQSW RNGVISGQVC PIKKLIKHSF ENSRVFALTE CDTVYYSYDN
GENWDYFTID HPISITQLPF HFHAKNPDYV IFNNQYCSSS GTWVGKICKP DLYYTKDGFQ
SDPEPMPVGS SYCIFADSSE KMVVSSEEQI ICISLNPNSA ARPPFSHHIV YSDDWFQSIV
PVQLHNFLGS DGAYGILSTG SFLVAALIDA ATRKLFVYVS QDGYYWEEAL KFHKGFEFDA
FTILPSTEYS FFIDSLDSHP NNPTGILYSL DSESNTFVIR QMNTNRYVDG YTDFMLIDYL
DGLQFVNVVE NVDEIEVDPQ VDKVLSSRIT FDGGKTWSTV ASPESSCNSM KQCSLHLFLD
PHVSHASIAS SKFAPGILLA SGSVGDRLLS ENQMDLFVSE DGGRNWTLSR DGMHLFAMSG
FGSIFFASEY LDVINEVYYS LDHGQSWVTV TLDKTIVPIK LFASEDPYAE IFYLLAMTDD
GEQSNYSLFS FNFGKFLPKE CQFSNSESNK NDFEKWYTRY ANGSPICSEM GKKEFFWRKK
ATSVCSVPKS ITDLHGSFDA CECTDEDYEC NTQFISNDQG ECKLLDFIGS LLCASEDLDT
FQKIPYRLVP GNKCTPNKRD SHREPQTFNC DSFNEPGTEI TSFLYDFDEK IVDVVYLEGT
VPEENTFLIG ISVNSHVYFS EDEGKTWDKF SKEEFSSVLP HAYNKNSVYM VTSKNIVYFT
TNRGKNFYKF KAPSPPNQNG KSLFSFHPSR PAWLLYAGSE NCEKNPFADD CRDVVFVSLD
FGDTWSRLPS NLEYCSWAKA EKLVVDDTLI FCIRQNTNDP FKKELISSID FFEYEQDEIL
NDVVGFMIED EYVIVAVQDE EGTSLSLDVS INGLNFASCS FPAYLNVHPK QAYTILDSQT
HSLFIHVTTN THLGSEWGDI LKSNSNGTYF MTSLANVNRD SVGYVDFERL EGIQGIALAN
IVSNTKELTD GGTKKLQTLI TFNDGLDWSY LNLVGGEKIV PKCGKNCYLH LHGYTERNQF
SDPTSTNAAV GLIIGVGSFS PFLIPYEESQ TFISRDAGVT WYRIFDSPHL WAFLDSGSII
IAVESISPTN VIKYSADEGR TWQEYQFSEK SKVVVDVSTK PSGVGHQVLL LTTDDENAPI
SSVLIDFDAL YRRTCVFDEE NSEESDFVRW VPTDISGKPL CLRGRISSFY RKSIHKKCRV
GSSLLVKEEV LSKCECTRAD FECDYNYRRL KDGTCVLVSG LQPPDTREEQ CSVDDAFEWR
QPTGYKRTPL TECEGGVPLD AGTLHPCPGK EDDYYKAHPK PGGWSIFLTI IFSILLAAVA
GCILYYYSRR FLKGAIRLGS DSATENPLES GISYTRGAFS SIPIFFSALY QSVRSLFIRS
TPTNGEFENA AFLQNYEIDD DDEESV
