VPS10_USTMA
ID VPS10_USTMA Reviewed; 1506 AA.
AC Q4P3I9; A0A0D1CH66;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 2.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; ORFNames=UMAG_10561;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC Prevacuolar compartment membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}. Note=Cycles between the Golgi apparatus
CC and the prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; CM003158; KIS66323.1; -; Genomic_DNA.
DR RefSeq; XP_011392160.1; XM_011393858.1.
DR AlphaFoldDB; Q4P3I9; -.
DR SMR; Q4P3I9; -.
DR STRING; 5270.UM05324P0; -.
DR EnsemblFungi; KIS66323; KIS66323; UMAG_10561.
DR GeneID; 23566577; -.
DR KEGG; uma:UMAG_10561; -.
DR VEuPathDB; FungiDB:UMAG_10561; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_0_1; -.
DR InParanoid; Q4P3I9; -.
DR OrthoDB; 1046610at2759; -.
DR Proteomes; UP000000561; Chromosome 19.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..1506
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407540"
FT TOPO_DOM 35..1383
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1384..1404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1405..1506
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 71..82
FT /note="BNR 1"
FT REPEAT 114..124
FT /note="BNR 2"
FT REPEAT 396..406
FT /note="BNR 3"
FT REPEAT 474..484
FT /note="BNR 4"
FT REPEAT 516..525
FT /note="BNR 5"
FT REPEAT 795..805
FT /note="BNR 6"
FT REPEAT 1181..1190
FT /note="BNR 7"
FT CARBOHYD 1010
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1299
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1506 AA; 168694 MW; 0B8408C52BD14AA7 CRC64;
MSTIPLRHSR RQWLPLFLLY LVSTLIILPA ATLAADAGVK VSRFAHLPSK VSYFDDSSVV
LYHDATAGVV YRSEDEGKTW SPVSGSSSGK AAMLVPHPYD KKQAFILSRE LTHWRSTDRG
KTWQEFLTPE PPATRAGPPM EFHADEKHWD WIIFTGKKCS MWTPWGGRIC HDEAYYTTNA
FATNPPRLLE FVMHCNWAKA TPEMQTSTDA LERIFCIAWE DAQEQRRSMV AGGTGGSTRL
FQSDDFFKSR KMVELDMGRN ARQFAGLGPS KRFLVTALRD SQGSSSKAGT EMALFVTKDG
VKWEKAKFPH GSELRENAYT VVDSTSHSIM IDVVDSVYDN TGVLFTSDST GTQFVESLRG
TRRTPQGLVD FEHLVKIDGV GIANVQSEAE GGVRSMITFD DGSSWHTLKP PAEDDKGKRI
DCDPSDAENC ALHLWSVSGK TNVGKLFSSV APGFVMGVGT IGKGLKNYDE CDTFLSIDAG
RTWKMVSRDA HKYEFGDQGS VLVMVDDEDV TDHVSYSTNF GKTWNKLSLG VTMRAKVLTT
IPDSTSLKFL LVGSQTRKQA GGKDRNVVIF LDFAEMKKSK CRESDMEKWY VQAAAEGSCL
MGHKQWYKRR KPDADCFIQD KFHDPEDKED PCPCTDADYE CDFGFVRDKQ GECVATGNEH
IPEGACAKPS DTYLGSSGYR KVPGNTCDAG KGIRKDEKTQ KSCSGKNMPQ KGSVLHKSFT
FPSLVVDKMY FPESSSVLVQ LADGTVFQSL NDGYSWKQLN GDGAPSSAED RFLTMALHAY
DPKRGYLITA GQRVYYTTNQ GASWSWFSVP LPANGLGINI LDFHPDRSDW LIWTGSRDCT
FSTAKQCHAE AWYSVSNGAK WEKIDSYVRT CSWARDKKLK MDARAIFCES YQDKKGNQRE
FTAANRLELI RGDEFYRKRT RVFDAVVGFA VFEQYLVVAE YVTTSSAPGL QLHVSLNGRD
FAPIHFPPGM QLGTRAYTVL DSVTDAIFLH FTTHSEAGSE WGTLLKSNSN GTFYTQSLDF
VNRNDKGFVD FEKMMGLDGV AIVNVVSNPD DASVSRQKDL VTRITHNDGG RWKSLVPPSR
DVYGQPYECN KVGCDLHLHG FTERDDPRDT YSSPSAVGLM MGVGNVGRKL APYRDSDTFL
TRDGGFSWEE VHKDAHKWEF GDQGSIIVLV NDEQATDSVL YSLNEGVTWE SYTFGERMRI
SQIVTVPEDT HRRFILVGTP SGSATKSVAI YLDFSALESR KCVLNVAKPE ADDFELWSPS
EERAEQCLFG RQTYYYRRKR RADCYVGEKI VQPHSVSRNC SCTEADFECE FNHYRDAKGT
CVLYPGVSPL STDAASQCWA ADSDGYWYER TNVRKIPYSW CTQGSRPDRG TRHVCSNNLR
AHGFLWWFFV IGCAFGLAGL VGYWWAKKQS SRPGYGSSGR IRLPDASNRL YGRDSELVQN
LASVPRFVLG AASAGFARFR EIASDRIPFV RNRLDRSRGA YGGYRHLSTD EDAAMLPDFE
EEEFER
