VPS10_VERA1
ID VPS10_VERA1 Reviewed; 1447 AA.
AC C9SM62;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE Flags: Precursor;
GN Name=VPS10; ORFNames=VDBG_05986;
OS Verticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136)
OS (Verticillium wilt of alfalfa) (Verticillium albo-atrum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Plectosphaerellaceae; Verticillium.
OX NCBI_TaxID=526221;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VaMs.102 / ATCC MYA-4576 / FGSC 10136;
RX PubMed=21829347; DOI=10.1371/journal.ppat.1002137;
RA Klosterman S.J., Subbarao K.V., Kang S., Veronese P., Gold S.E.,
RA Thomma B.P.H.J., Chen Z., Henrissat B., Lee Y.-H., Park J.,
RA Garcia-Pedrajas M.D., Barbara D.J., Anchieta A., de Jonge R., Santhanam P.,
RA Maruthachalam K., Atallah Z., Amyotte S.G., Paz Z., Inderbitzin P.,
RA Hayes R.J., Heiman D.I., Young S., Zeng Q., Engels R., Galagan J.,
RA Cuomo C.A., Dobinson K.F., Ma L.-J.;
RT "Comparative genomics yields insights into niche adaptation of plant
RT vascular wilt pathogens.";
RL PLoS Pathog. 7:E1002137-E1002137(2011).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; DS985220; EEY19877.1; -; Genomic_DNA.
DR RefSeq; XP_003003544.1; XM_003003498.1.
DR AlphaFoldDB; C9SM62; -.
DR SMR; C9SM62; -.
DR STRING; 526221.C9SM62; -.
DR PRIDE; C9SM62; -.
DR EnsemblFungi; EEY19877; EEY19877; VDBG_05986.
DR GeneID; 9536344; -.
DR KEGG; val:VDBG_05986; -.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_000700_0_0_1; -.
DR OMA; ATMSEFI; -.
DR Proteomes; UP000008698; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 3.
DR SMART; SM00602; VPS10; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..1447
FT /note="Vacuolar protein sorting/targeting protein 10"
FT /id="PRO_0000407542"
FT TOPO_DOM 27..1314
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1315..1335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1336..1364
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1365..1385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1386..1447
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REPEAT 65..74
FT /note="BNR 1"
FT REPEAT 108..119
FT /note="BNR 2"
FT REPEAT 386..396
FT /note="BNR 3"
FT REPEAT 447..458
FT /note="BNR 4"
FT REPEAT 491..500
FT /note="BNR 5"
FT REPEAT 723..733
FT /note="BNR 6"
FT REPEAT 820..829
FT /note="BNR 7"
FT REPEAT 1103..1113
FT /note="BNR 8"
FT REGION 1426..1447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1433..1447
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 306
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 331
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 864
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 973
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1447 AA; 161426 MW; A1B6515434F32578 CRC64;
MRIWGRGAAS WRTLLFVLSW TTTTLAKDDG PTIKVSKFKH PPLNMNYFED SDVVLFHDMS
ENNIYRSDDA GVSWGKVAGV PDGVAYTLAM HEFDNSRAFI ITDRREHFKT TDRGKSWEKF
DSAAQTSRYR DQILSFHAGD PDRVIFNGMD CAGIFCNEVA TYTTNNFKEA KTLRGSTEGC
WWAKSSELFT TGKDDLDRQR VLCIGADSIS PFKEDQRLFI SDNFFEKIDG QVQQFEPNLD
TNHGIQGVVN LAVVKKYLLV ATTSINTDEM SLFVTDDTLK WHRAMFPAAH GHKVNQGAYT
VLEGTNYSIQ VDVMNTRPSN PMGVMFTSNS NGTFFVENLE HTNRNERGNV DFEKISGIQG
IFLVNTVKNW EDVEKHGGSR KEVVTQITFD DGRTFHDIKA GDDRVHLHSV THLDNVGRVF
SSPAPGLVMG VGNTGGSLND FEEGNLFVSD DAGASWKKAL DGPHKYEFGD QGSLLVAVKD
SKKDDVGEVS YSLDHGLNWK TAALPDGLKL KPEIITTTQD STSLKFILVG SSGGDNYHVV
ALDFGGLQEK TCEDNDMEDW PARDADCFLK KEFKDPIPKT EDCECSDADF ECDYNFIRKD
GECEKAGPIV APDEACKNAG PDTSFKGSSG WRLIPGNTCK RKDGSQKDDP VDRKCSDVVN
SPAPPASGDI TATQHIFKDT KLNDFEKIYL ERGDSSSDSD ETVIARAVEY ENDGSMRVEQ
RVYRTRDHGK KWDEILEKEE IRGIYPHQYF KDAVFFTTKS RKVWYTIDRA EHFHEFEAPS
DPGSGNPLSF HPDKKDWLIW VGQKCEKVDG KEQCFKEASI SRDRGDNWRT ALRYVQKCEF
TGRSTYKFRD MRQIVCLTHK REDNESDNPK VIVSSNDFFE EDKQVRESNV KDFATMAEFI
VVAAEDKEQK GLRALASLDG ETYAAAHFPY NFEVSHQNAY TVLDSSTHAV NLFVATQLEK
NQRQGSILKS NSNGTSYVMS APKVNCDNNY FVDFEKIIGL EGVVLINSVT GKDKKGNKIL
RTEISHNDGS QWAYLPPPKA DVNGKAYGCS STYGDEKCAL HLHHYTERLD KKKTFSAASV
AGLMFGYGNI GSSLGPIEEA DTFMTTDAGI TWKSVKKGAW TWQYGDQGSI VVLVPRSTED
KKAKTKSISY TTNEGKDWKD FEFSQEEVTI LDITSIRTGS SRNFLLWCRS SDGKTFSVNL
DFTGLTDRPC VNPESGDSDY YLWSPTHPLQ DNECDYNFEL DPHKQCSLVP GKKPLSAEQY
CKENPDAVSY YEPTGYRRIP LTTCVGGTEL DKISEAHPCK GKEEEFEKLR GTSGVAIFFA
IVIPIGIAAA VGWWVWRNYG SQFGQIRLGE SSGFDNDAPW VKYPVIAVSA AVAVVAALPL
LGSALWRTAS SAYERVSGGG SGGGSWLNGG GQRRFTTRDS FARGRGDYAS VDDDEGELLG
DDSDEEV
