VPS10_YEAS7
ID VPS10_YEAS7 Reviewed; 1579 AA.
AC A6ZKT1;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Vacuolar protein sorting/targeting protein PEP1;
DE AltName: Full=Carboxypeptidase Y receptor;
DE Short=CPY receptor;
DE AltName: Full=Carboxypeptidase Y-deficient protein 1;
DE AltName: Full=Sortilin VPS10;
DE AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE AltName: Full=Vacuolar protein sorting-associated protein 10;
DE AltName: Full=Vacuolar protein-targeting protein 1;
DE Flags: Precursor;
GN Name=PEP1; Synonyms=VPS10, VPT1; ORFNames=SCY_0201;
OS Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=307796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YJM789;
RX PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA Steinmetz L.M.;
RT "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT strain YJM789.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC required for the intracellular sorting and delivery of soluble vacuolar
CC proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC multiple rounds of sorting by cycling between the late Golgi and a
CC prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form
CC of CPY, and this interaction is dependent on the presence of an intact
CC CPY vacuolar protein sorting signal (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC Prevacuolar compartment membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}. Note=Cycles between the Golgi apparatus
CC and the prevacuolar compartment. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS10-related sortilin family.
CC {ECO:0000305}.
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DR EMBL; AAFW02000011; EDN64600.1; -; Genomic_DNA.
DR AlphaFoldDB; A6ZKT1; -.
DR SMR; A6ZKT1; -.
DR PRIDE; A6ZKT1; -.
DR EnsemblFungi; EDN64600; EDN64600; SCY_0201.
DR HOGENOM; CLU_000700_0_1_1; -.
DR Proteomes; UP000007060; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR036278; Sialidase_sf.
DR InterPro; IPR031777; Sortilin_C.
DR InterPro; IPR031778; Sortilin_N.
DR InterPro; IPR006581; VPS10.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF15902; Sortilin-Vps10; 2.
DR Pfam; PF15901; Sortilin_C; 2.
DR SMART; SM00602; VPS10; 2.
DR SUPFAM; SSF50939; SSF50939; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW Repeat; Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..1579
FT /note="Vacuolar protein sorting/targeting protein PEP1"
FT /id="PRO_0000407546"
FT TOPO_DOM 22..1393
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1394..1414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1415..1579
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 58..68
FT /note="BNR 1"
FT REPEAT 101..111
FT /note="BNR 2"
FT REPEAT 181..189
FT /note="BNR 3"
FT REPEAT 416..425
FT /note="BNR 4"
FT REPEAT 487..497
FT /note="BNR 5"
FT REPEAT 533..543
FT /note="BNR 6"
FT REPEAT 764..774
FT /note="BNR 7"
FT REPEAT 861..871
FT /note="BNR 8"
FT REPEAT 1143..1152
FT /note="BNR 9"
FT REPEAT 1185..1194
FT /note="BNR 10"
FT REGION 1533..1579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 447
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 793
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1010
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1303
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1579 AA; 177705 MW; A1BB81D8D426F26C CRC64;
MILLHFVYSL WALLLIPLIN AEEFTPKVTK TIAQDSFEIL SFDDSNTLIR KQDTSVTISF
DDGETWEKVE GIEGEITWIY IDPFNRHDRA VATAMNGSYL YITNDQGKSW ERITLPDSGE
SISPRGCYIE THPLNKNYFL AKCNYCEKTE VNNDNEENSG DEEGEFEIFN ITRCTDKVFA
SNDGGKSFSE IKSSLERNEN SPISISDCGF AKTSKDSDLE SSDTSIICLF QNMQFIMDEF
SSPYTESKLV LTTDWGKSLK EFDQFKDKVV NGYRILKSHM VVLTQGDRYN DMSSMDVWVS
NDLSSFKMAY MPTQLRHSMQ GEIYEDAMGR IILLMSRERS DQEEDKGIVS EILISDSQGL
KFSPIPWTAN EVFGYINLYQ PTYLKGTMIA SLYPLSRRRN RKGKAKGVKS KGVTKISVDN
GLTWTMLKVV DPDNADSFDC DITDFENCSL QNMFYTREGS TPTAGILMTT GIVGDGSVFD
WGDQRTFISR DGGLTWKLAF DFPCLYAVGD YGNVIVAIPY NADEDDDPQS EFYYSLDQGK
TWTEYQLETT IYPNEVMNTT PDGSGAKFIL NGFTLAHMDG TTNFIYAIDF STAFNDKTCE
ENDFEDWNLA EGKCVNGVKY KIRRRKQDAQ CLVKKVFEDL QLFETACDKC TEADYECAFE
FVRDATGKCV PDYNLIVLSD VCDKTKKKTV PVKPLQLVKG DKCKKPMTVK SVDISCEGVP
KKGTNDKEIV VTENKFDFKI QFYQYFDTVT DESLLMINSR GEAYISHDGG QTIKRFDSNG
ETIIEVVFNP YFNSSAYLFG SKGSIFSTHD RGYSFMTAKL PEARQLGMPL DFNAKAQDTF
IYYGGKNCES ILSPECHAVA YLTNDGGETF TEMLDNAIHC EFAGSLFKYP SNEDMVMCQV
KEKSSQTRSL VSSTDFFQDD KNTVFENIIG YLSTGGYIIV AVPHENNELR AYVTIDGTEF
AEAKFPYDED VGKQEAFTIL ESEKGSIFLH LATNLVPGRD FGNLLKSNSN GTSFVTLEHA
VNRNTFGYVD FEKIQGLEGI ILTNIVSNSD KVAENKEDKQ LKTKITFNEG SDWNFLKPPK
RDSEGKKFSC SSKSLDECSL HLHGYTERKD IRDTYSSGSA LGMMFGVGNV GPNLLPYKEC
STFFTTDGGE TWAEVKKTPH QWEYGDHGGI LVLVPENSET DSISYSTDFG KTWKDYKFCA
DKVLVKDITT VPRDSALRFL LFGEAADIGG SSFRTYTIDF RNIFERQCDF DITGKESADY
KYSPLSSKSN CLFGHQTEFL RKTDENCFIG NIPLSEFSRN IKNCSCTRQD FECDYNFYKA
NDGTCKLVKG LSPANAADVC KKEPDLIEYF ESSGYRKIPL STCEGGLKLD APSSPHACPG
KEKEFKEKYS VSAGPFAFIF ISILLIIFFA AWFVYDRGIR RNGGFARFGE IRLGDDGLIE
NNNTDRVVNN IVKSGFYVFS NIGSLLQHTK TNIAHAISKI RGRFGNRTGP SYSSLIHDQF
LDEADDLLAG HDEDANDLSS FMDQGSNFEI EEDDVPTLEE EHTSYTDQPT TTDVPDALPE
GNEENIDRPD STAPSNENQ
