ID   VPS13_DROME             Reviewed;        3321 AA.
AC   A1Z713;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Intermembrane lipid transfer protein Vps13 {ECO:0000305};
DE   AltName: Full=Vacuolar protein sorting-associated protein 13 {ECO:0000303|PubMed:28107480};
GN   Name=Vps13 {ECO:0000303|PubMed:28107480, ECO:0000312|FlyBase:FBgn0033194};
GN   ORFNames=CG2093 {ECO:0000312|FlyBase:FBgn0033194};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN   [1] {ECO:0000312|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2] {ECO:0000312|Proteomes:UP000000803}
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=28107480; DOI=10.1371/journal.pone.0170106;
RA   Vonk J.J., Yeshaw W.M., Pinto F., Faber A.I., Lahaye L.L., Kanon B.,
RA   van der Zwaag M., Velayos-Baeza A., Freire R., van Ijzendoorn S.C.,
RA   Grzeschik N.A., Sibon O.C.;
RT   "Drosophila Vps13 Is Required for Protein Homeostasis in the Brain.";
RL   PLoS ONE 12:E0170106-E0170106(2017).
CC   -!- FUNCTION: Mediates the transfer of lipids between membranes at
CC       organelle contact sites (By similarity). Plays a role in the survival
CC       of neurons by maintaining protein homeostasis in the central nervous
CC       system (PubMed:28107480). May function as part of a lysosomal
CC       degradation pathway (PubMed:28107480). {ECO:0000250|UniProtKB:Q07878,
CC       ECO:0000269|PubMed:28107480}.
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane; Peripheral membrane
CC       protein {ECO:0000269|PubMed:28107480}.
CC   -!- TISSUE SPECIFICITY: High levels in the central nervous system (at
CC       protein level). {ECO:0000269|PubMed:28107480}.
CC   -!- SIMILARITY: Belongs to the VPS13 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE013599; AAF59228.2; -; Genomic_DNA.
DR   EMBL; AE013599; AGB93314.1; -; Genomic_DNA.
DR   RefSeq; NP_001260781.1; NM_001273852.1.
DR   RefSeq; NP_610299.2; NM_136455.3.
DR   IntAct; A1Z713; 1.
DR   STRING; 7227.FBpp0087984; -.
DR   PaxDb; A1Z713; -.
DR   PRIDE; A1Z713; -.
DR   EnsemblMetazoa; FBtr0088910; FBpp0087984; FBgn0033194.
DR   EnsemblMetazoa; FBtr0310024; FBpp0301727; FBgn0033194.
DR   GeneID; 35693; -.
DR   KEGG; dme:Dmel_CG2093; -.
DR   UCSC; CG2093-RA; d. melanogaster.
DR   CTD; 35693; -.
DR   FlyBase; FBgn0033194; Vps13.
DR   VEuPathDB; VectorBase:FBgn0033194; -.
DR   eggNOG; KOG1809; Eukaryota.
DR   GeneTree; ENSGT00950000183083; -.
DR   HOGENOM; CLU_000135_1_1_1; -.
DR   InParanoid; A1Z713; -.
DR   OMA; VDVRDWS; -.
DR   OrthoDB; 4159at2759; -.
DR   PhylomeDB; A1Z713; -.
DR   BioGRID-ORCS; 35693; 0 hits in 3 CRISPR screens.
DR   ChiTaRS; Vps13; fly.
DR   GenomeRNAi; 35693; -.
DR   PRO; PR:A1Z713; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0033194; Expressed in oviduct (Drosophila) and 25 other tissues.
DR   GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR   GO; GO:0031902; C:late endosome membrane; IDA:FlyBase.
DR   GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR   GO; GO:0019538; P:protein metabolic process; IMP:FlyBase.
DR   GO; GO:0045053; P:protein retention in Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0006623; P:protein targeting to vacuole; ISS:FlyBase.
DR   InterPro; IPR026847; VPS13.
DR   InterPro; IPR026854; VPS13-like_N.
DR   InterPro; IPR031645; VPS13_C.
DR   InterPro; IPR031642; VPS13_mid_rpt.
DR   InterPro; IPR031646; VPS13_N2.
DR   InterPro; IPR009543; VPS13_VAB.
DR   PANTHER; PTHR16166; PTHR16166; 1.
DR   Pfam; PF12624; Chorein_N; 1.
DR   Pfam; PF06650; SHR-BD; 1.
DR   Pfam; PF16908; VPS13; 1.
DR   Pfam; PF16909; VPS13_C; 1.
DR   Pfam; PF16910; VPS13_mid_rpt; 2.
PE   1: Evidence at protein level;
KW   Endosome; Lipid transport; Membrane; Reference proteome; Transport.
FT   CHAIN           1..3321
FT                   /note="Intermembrane lipid transfer protein Vps13"
FT                   /id="PRO_0000440688"
FT   DOMAIN          3..116
FT                   /note="Chorein N-terminal"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          2351..2598
FT                   /note="SHR-BD"
FT                   /evidence="ECO:0000255"
FT   REGION          1399..1425
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   3321 AA;  374545 MW;  3FBE8B43812C3FF9 CRC64;
     MVFEAVVADV LNKVLGDYIE NLDRNQLKIG IWGGDVVLQN LKIRENALDE LDLPVQLIYG
     YLGKLVLKIP WKNLYSQPVI VNIEDLYVLV SPNNNVQYNA EKEAKYEMDL KKAALDALEA
     ARKKELEMDQ PKADAGFAEK LTAQIVNNLQ VQITNVHLRY EDTTTTGSPF SFGISLHELE
     LYTTDCDWEK CYMAQQASQV FKIANLSCLS AYLNCGGQLY ANNKSDLSQQ FKTNIACKET
     KPNYNYVLGP ISCNAKLKLN MNPELDDPPF EKPKIDLTLE MEKLNVGLTN TQFDNLMKLG
     DAMNRQQLGI PYRKYRPYNI PYKGHARDWW HFAITSILEE EVRKPRESWT WGHIKTHRER
     CNTYAQKYKE QCLSKKPSAV LTETCRLLET ELDVFNLLLI RQRVNIEIAK QREAVPEQKS
     GWFSGWGWGG GAKKDDQTTS QKLVEKFEAA MTSEEKEKMY RAIGYQENAK PTDLPESYEA
     IRMNFKLIAL EVGLYKDERN SSAATKDFHE LPSLVLLNFS MATALITQRP AAEAISIIAG
     MREIKVTGLT RNDYTPLLVE SKITDEFNLL EVFFETNPLD KLCDQRVKVV ARPLQITYDA
     PTILALINAF QTPGDVTLSK FEDAASTKIS NFKERSATGM QYMIDKKAVL DVDILLMPNI
     LVVPHKGVYD AGNVSLLVVS MGQVHLSSQP RRESNKLQHL FSAGEDKDEI LKTVMENAYD
     RFTVAVDDVQ MLVVRAGEPW QNALAEANST EMHVLRPVSL KVTAALCVVD NDPRLPNIKV
     DIDLPAILVN VSEDRIFLAI KVATSIPLPE QKEPASRLTQ TNSRSSMSIS NFINKEVKKI
     GPSASGSSAS KDPLLDEIIQ YTSLDVNFSL GEINFVLFQS SRKCETSPDV SIEFLTPDGD
     VLPSQLTENI QEPIEELPPT PPQQILSIDI RRLEAHFVSK TYESVATVKL GDINLRQYDC
     QDSDMDVLDV IYTPKQENSS NYLFTVSCTI ADKSSPEFST KYNSTEQLVV ANFEVLQIVL
     HQECLQRIME VVNNFQRNLD LVLSSTRPRD RMGSIGGGDG IKRTLNVILE DTEEIMTTDQ
     MKRRKKTRRT HVVETVKVRV IANLDQVGLV LTGRKRPIAE MNVKKFVSSL IIKSSYTEVN
     IGLKDIQVLD LNPYTIHKNI LSIVGKDAFN CQIVIYNKEE TQDYNSDDMK ITVDIGCMKI
     IFLNWFVAGV MNFLNNFTAA QATISQAGAA AAESARQKAM DAYETATRMK LNIRIKAPII
     IVPIGSQDRN ALLLDLGLLE LTNNTVEVAV AEEERLAVID EIKLQICDVK ISKIVLLDGN
     ESTVDEVDAE VGFLSKFNMM NPMSCTLSIT RNLSYTWYRD VPELNLSGRL KSIELTLFAD
     DYALVMLVLN RNLNEGLEEF PPSEEAPQEA QVRPERRNSR AGRLSRTVQV SPIREKIHES
     IKFNFQFDGV VINLMEGEGA GLARFGIYFL SVKGTKLDNG TLSTSVVLCN IQMDDMRSNS
     KSQIRQYLSR KDWVQPKLDT DEIIDACYNE RNFMVDVTAI IKEDDTFAEV RVRGFDLIVC
     IDFLLKLTTF LTLPPEENPR ESVYIKPAPV SETARDTKHS IRSSAILAAQ ELVPVESSSH
     EVPNRKMNLI LHIDEPDIIL VENLEDLNTS CIIFNAQVHL NYRSINDKQI VNGQIDALKM
     YMCAFLPERR EMTRHYILHP CVISLQGSTP EEEGMHISLK LSDIIINVSP ATIELLNKAM
     LSVSSGTMTK CAIAEESRNY SNLWHQHHFH SRTYWFTKVE QGVDALEAEQ RSVSTDNEKQ
     KTEKCVIEIP SITLVIESGV GYYTKPLISL DTRITAVFNN WSRSLTAHGS LTLNMNYYNQ
     ALAEWEPIIE LNEVIGRNGV REYTPWELKF EMGMEKVQSE LEDDAEQQAM HMNIHSAETL
     EITLSKTCLG LLSELAEAFS QAIDQNGLTK PDIVAPYVLE NDTGFDVNLN LRKGIFTLHE
     VHRGGTPVGA NSTLLMVAQS EEVDPSVIKT CTISTGGRAY LQTKDLSTLS EEDSEDYTLY
     VTIGDINKEI ALPVSKSDTR FFNLMRSTSH EPWGIISEVK QEYGTTKVNI HGVVSVHNHF
     TTGLNIYRRN PAPTAQCFED IFVGRVRPGE VFHVPLHAIY AESKDLFFSM RGYRRSVQGI
     SWASNPSDLN YSHQLHCDPT NTFEPLIMNA RRSKSEVYFE NTNKYTLLSA FYTIHLRPPL
     YLRNSLPINI QVSVAGCSVR KEDGLDAQSS QRFVDRGYRK EDFLDYGEKP VNSGDVLHLP
     TVRLASKGKE SKSFLVVRLV QYLEKDWSCA TEIWDYTDDV ITWTFSSYDS EMKVDMDLYV
     KTENRHGSLM LTLFSPFWMI NKTGMMLTYK SETTSVEVLY HPPEYSGPIL FTFRDKLFFD
     KKKASIRIDN GQWSEKIPLD VAGSVGEVIC FANNQKYPVG VHNHLTQNSL TKQITFIPFY
     IVCNKCHFDI ELQEQSRPAD PWLHLEPNEM EPLWPRNDTK NNLVVRVDGK ITPAFDFTEV
     ICTLLKLEDS KYGGINVDVQ TTEGGVYITF TDYKPADAPG LLINHTGKQI VYHEKGTKNE
     HILNAKSTIM YAWDDPTGPK MLVFGTNKEE TDLKRDGIGE VIMQDGGKVL WVSFLDGLQR
     VLLFTENESI ANRTESTASL QSITQSIDLR IHGIGLSVIN NETGLDILYL GVTSSGIIWE
     SKKVTKNRFK ELTINENALL EIEYQKYLVH KSVNDVQTYK LDNKFPIDFD LMILKKTVER
     NLRRSFYPAI WLSRKSSPFQ SQLHVKINRI QVDNQFLDPI FPVVLAPIPP PKSVASTTSL
     KPFIECSMVQ RIMPNSTVRQ FKYARILIQE FLFKVDLNFL TAIAEMFAKE VSDEAAAKQF
     RQDVESIELP LSAFFEEHSL EEQKSFYDNL HLGPLKIHVS FSMAGSDTKA LPGFLGSLVQ
     GVGVTLTDVN DVVFRLAFFE REYQFFSQKQ LINEITSHYT GQALKQLYVL VLGLDVLGNP
     YGLVVGLKKG VEDLFYEPFQ GAIQGPGEFA EGLVLGVKSL FGHTVGGAAG AVSKITGAMG
     KGLAALTFDE DYQKKRRQGI QNKPKNFHEG LARSSKGLVM GFVDGVTGVV TKPVTGARDN
     GVEGFFKGLG KGAIGLVARP TAGVVDFASG SFEAVKRAAD ASEDVKRMRP PRFQHYDFVL
     RPYCLMEATG NKIMKETDKG KFATTDNFIH CEEIIQKSEY LVVTNYRVMY VQRNEMFGVW
     TSLWSYLWNE ISSVAATARG VQFTVKTDGK KVLGLFSSKE SPRKLVLVAD ERKRDALVDI
     IESQRSDPNP LRATIAYPAH N