位置:首页 > 蛋白库 > VPS18_MOUSE
VPS18_MOUSE
ID   VPS18_MOUSE             Reviewed;         973 AA.
AC   Q8R307; Q8BGV6; Q8BZX6; Q8C126;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2004, sequence version 2.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Vacuolar protein sorting-associated protein 18 homolog;
GN   Name=Vps18;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Skin, Testis, and Vagina;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Kidney, and Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION.
RX   PubMed=14517315; DOI=10.1091/mbc.e03-01-0040;
RA   Poupon V., Stewart A., Gray S.R., Piper R.C., Luzio J.P.;
RT   "The role of mVps18p in clustering, fusion, and intracellular localization
RT   of late endocytic organelles.";
RL   Mol. Biol. Cell 14:4015-4027(2003).
RN   [4]
RP   INTERACTION WITH HOOK1.
RX   PubMed=14668490; DOI=10.1091/mbc.e03-06-0358;
RA   Richardson S.C.W., Winistorfer S.C., Poupon V., Luzio J.P., Piper R.C.;
RT   "Mammalian late vacuole protein sorting orthologues participate in early
RT   endosomal fusion and interact with the cytoskeleton.";
RL   Mol. Biol. Cell 15:1197-1210(2004).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC   Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [6]
RP   SUBUNIT.
RX   PubMed=21411634; DOI=10.1091/mbc.e10-10-0799;
RA   Zlatic S.A., Tornieri K., L'Hernault S.W., Faundez V.;
RT   "Clathrin-dependent mechanisms modulate the subcellular distribution of
RT   class C Vps/HOPS tether subunits in polarized and nonpolarized cells.";
RL   Mol. Biol. Cell 22:1699-1715(2011).
RN   [7]
RP   FUNCTION.
RX   PubMed=22699122; DOI=10.1016/j.bbrc.2012.06.021;
RA   Peng C., Yan S., Ye J., Shen L., Xu T., Tao W.;
RT   "Vps18 deficiency inhibits dendritogenesis in Purkinje cells by blocking
RT   the lysosomal degradation of Lysyl Oxidase.";
RL   Biochem. Biophys. Res. Commun. 423:715-720(2012).
RN   [8]
RP   FUNCTION.
RX   PubMed=22854957; DOI=10.1074/jbc.m112.384305;
RA   Peng C., Ye J., Yan S., Kong S., Shen Y., Li C., Li Q., Zheng Y., Deng K.,
RA   Xu T., Tao W.;
RT   "Ablation of vacuole protein sorting 18 (Vps18) gene leads to
RT   neurodegeneration and impaired neuronal migration by disrupting multiple
RT   vesicle transport pathways to lysosomes.";
RL   J. Biol. Chem. 287:32861-32873(2012).
RN   [9]
RP   SUBUNIT, AND INTERACTION WITH RAB5C.
RX   PubMed=25266290; DOI=10.1111/tra.12232;
RA   Perini E.D., Schaefer R., Stoeter M., Kalaidzidis Y., Zerial M.;
RT   "Mammalian CORVET is required for fusion and conversion of distinct early
RT   endosome subpopulations.";
RL   Traffic 15:1366-1389(2014).
CC   -!- FUNCTION: Plays a role in vesicle-mediated protein trafficking to
CC       lysosomal compartments including the endocytic membrane transport and
CC       autophagic pathways. Believed to act as a core component of the
CC       putative HOPS and CORVET endosomal tethering complexes which are
CC       proposed to be involved in the Rab5-to-Rab7 endosome conversion
CC       probably implicating MON1A/B, and via binding SNAREs and SNARE
CC       complexes to mediate tethering and docking events during SNARE-mediated
CC       membrane fusion. The HOPS complex is proposed to be recruited to Rab7
CC       on the late endosomal membrane and to regulate late endocytic,
CC       phagocytic and autophagic traffic towards lysosomes. The CORVET complex
CC       is proposed to function as a Rab5 effector to mediate early endosome
CC       fusion probably in specific endosome subpopulations (By similarity).
CC       Required for fusion of endosomes and autophagosomes with lysosomes
CC       (PubMed:14517315, PubMed:22854957). Involved in dendrite development of
CC       Pukinje cells (PubMed:22699122). {ECO:0000250|UniProtKB:Q9P253,
CC       ECO:0000269|PubMed:14517315, ECO:0000269|PubMed:22699122,
CC       ECO:0000269|PubMed:22854957}.
CC   -!- SUBUNIT: Core component of at least two putative endosomal tethering
CC       complexes, the homotypic fusion and vacuole protein sorting (HOPS)
CC       complex and the class C core vacuole/endosome tethering (CORVET)
CC       complex. Their common core is composed of the class C Vps proteins
CC       VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with
CC       VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with
CC       RAB5C (PubMed:25266290). Interacts with HOOK1 (PubMed:14668490).
CC       Interacts with STX7, MON1B (By similarity). Associates with adaptor
CC       protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).
CC       Interacts with SYNPO2 (By similarity). Interacts with PLEKHM1 (By
CC       similarity). {ECO:0000250|UniProtKB:Q9P253,
CC       ECO:0000269|PubMed:14668490, ECO:0000269|PubMed:21411634,
CC       ECO:0000269|PubMed:25266290}.
CC   -!- INTERACTION:
CC       Q8R307; Q9H9C1: VIPAS39; Xeno; NbExp=5; IntAct=EBI-2527788, EBI-749080;
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane
CC       {ECO:0000250|UniProtKB:Q9P253}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9P253}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q9P253}. Lysosome membrane
CC       {ECO:0000250|UniProtKB:Q9P253}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9P253}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q9P253}. Early endosome
CC       {ECO:0000250|UniProtKB:Q9P253}. Cytoplasmic vesicle, autophagosome
CC       {ECO:0000305}. Cytoplasmic vesicle, clathrin-coated vesicle
CC       {ECO:0000305}. Note=Cytoplasmic, peripheral membrane protein associated
CC       with early endosomes and late endosomes/lysosomes.
CC   -!- SIMILARITY: Belongs to the VPS18 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK029109; BAC26302.1; -; mRNA.
DR   EMBL; AK033333; BAC28236.1; -; mRNA.
DR   EMBL; AK036915; BAC29637.1; -; mRNA.
DR   EMBL; BC026870; AAH26870.1; -; mRNA.
DR   EMBL; BC036129; AAH36129.1; -; mRNA.
DR   EMBL; BC039176; AAH39176.1; -; mRNA.
DR   CCDS; CCDS16599.1; -.
DR   RefSeq; NP_758473.3; NM_172269.3.
DR   AlphaFoldDB; Q8R307; -.
DR   BioGRID; 230740; 19.
DR   IntAct; Q8R307; 5.
DR   MINT; Q8R307; -.
DR   STRING; 10090.ENSMUSP00000036915; -.
DR   iPTMnet; Q8R307; -.
DR   PhosphoSitePlus; Q8R307; -.
DR   EPD; Q8R307; -.
DR   MaxQB; Q8R307; -.
DR   PaxDb; Q8R307; -.
DR   PeptideAtlas; Q8R307; -.
DR   PRIDE; Q8R307; -.
DR   ProteomicsDB; 297582; -.
DR   Antibodypedia; 23197; 160 antibodies from 23 providers.
DR   DNASU; 228545; -.
DR   Ensembl; ENSMUST00000037280; ENSMUSP00000036915; ENSMUSG00000034216.
DR   GeneID; 228545; -.
DR   KEGG; mmu:228545; -.
DR   UCSC; uc008lto.2; mouse.
DR   CTD; 57617; -.
DR   MGI; MGI:2443626; Vps18.
DR   VEuPathDB; HostDB:ENSMUSG00000034216; -.
DR   eggNOG; KOG2034; Eukaryota.
DR   GeneTree; ENSGT00940000153635; -.
DR   HOGENOM; CLU_003488_1_0_1; -.
DR   InParanoid; Q8R307; -.
DR   OMA; HVQRESR; -.
DR   OrthoDB; 87842at2759; -.
DR   PhylomeDB; Q8R307; -.
DR   TreeFam; TF105704; -.
DR   BioGRID-ORCS; 228545; 23 hits in 75 CRISPR screens.
DR   ChiTaRS; Vps18; mouse.
DR   PRO; PR:Q8R307; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q8R307; protein.
DR   Bgee; ENSMUSG00000034216; Expressed in ankle joint and 175 other tissues.
DR   Genevisible; Q8R307; MM.
DR   GO; GO:0005884; C:actin filament; IDA:MGI.
DR   GO; GO:0005776; C:autophagosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0033263; C:CORVET complex; IDA:UniProtKB.
DR   GO; GO:0005769; C:early endosome; IDA:MGI.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR   GO; GO:0030897; C:HOPS complex; ISO:MGI.
DR   GO; GO:0005770; C:late endosome; ISO:MGI.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISO:MGI.
DR   GO; GO:0098793; C:presynapse; ISO:MGI.
DR   GO; GO:0003779; F:actin binding; IDA:MGI.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IBA:GO_Central.
DR   GO; GO:0019905; F:syntaxin binding; ISO:MGI.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; ISO:MGI.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0007032; P:endosome organization; IDA:MGI.
DR   GO; GO:0008333; P:endosome to lysosome transport; ISO:MGI.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   GO; GO:0007040; P:lysosome organization; ISO:MGI.
DR   GO; GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; ISO:MGI.
DR   GO; GO:0048284; P:organelle fusion; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; ISO:MGI.
DR   GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; ISO:MGI.
DR   GO; GO:0007033; P:vacuole organization; IBA:GO_Central.
DR   GO; GO:0006904; P:vesicle docking involved in exocytosis; IBA:GO_Central.
DR   GO; GO:0046718; P:viral entry into host cell; IMP:MGI.
DR   InterPro; IPR000547; Clathrin_H-chain/VPS_repeat.
DR   InterPro; IPR007810; Pep3_Vps18.
DR   Pfam; PF00637; Clathrin; 1.
DR   Pfam; PF05131; Pep3_Vps18; 1.
DR   PROSITE; PS50236; CHCR; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Autophagy; Coiled coil; Cytoplasmic vesicle; Endosome;
KW   Lysosome; Membrane; Metal-binding; Phosphoprotein; Protein transport;
KW   Reference proteome; Transport; Zinc; Zinc-finger.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   CHAIN           2..973
FT                   /note="Vacuolar protein sorting-associated protein 18
FT                   homolog"
FT                   /id="PRO_0000055907"
FT   REPEAT          618..772
FT                   /note="CHCR"
FT   ZN_FING         853..947
FT                   /note="RING-type"
FT   COILED          454..481
FT                   /evidence="ECO:0000255"
FT   COILED          802..848
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         3
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         11
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         13
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         362
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         689
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   MOD_RES         912
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9P253"
FT   CONFLICT        676
FT                   /note="P -> T (in Ref. 1; BAC28236)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        943
FT                   /note="E -> G (in Ref. 1; BAC26302)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   973 AA;  110219 MW;  6B4E90FC39DC897F CRC64;
     MASILDEYED SLSRSAVLQT GCPSVGIPHS GYVSAHLEKE VPIFTKQRVD FTPSERITSL
     VVSCNQLCMS LGKDTLLRID LGKASEPNRV ELGRKDDAKV HKMFLDHTGS HLLVALSSTE
     VLYMNRNGQK ARPLARWKGQ LVESVGWNKA MGNESSTGPI LVGTAQGQIF EAELSASEGG
     LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRGFV IATTRQRLFQ FIGRAVEDTE
     AQGFAGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGSLDCG
     RPDSLLSEER VWEYPAGVGP GANPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL
     EKFGPLRHMV KDSSTGHLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD
     CLDTVLAREA DFCFRQHRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLAGL
     KPTERTQATL LTTWLTELYL SRLGALQGDP DALTLYRDTR ECFRTFLSSP RHKEWLFASR
     ASIHELLASH GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP
     ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEAQ QVSQAIRYME FCVNVLGETE
     QAIHNYLLSL YARGQPASLL AYLEQAGASP HRVHYDLKYA LRLCAEHGHH RACVHVYKVL
     ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE LRKKLWLKIA RHVVQEEEDV QTAMACLASC
     PLLKIEDVLP FFPDFVTIDH FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL
     RGRYGTVEPQ DKCSTCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR
     KLGAAPPPTK GSVKAKEAEA GAAAVGPSRE QLKADLDELV AAECVYCGEL MIRSIDRPFI
     DPQRYEEEHL SWL
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024