VPS20_SCHPO
ID VPS20_SCHPO Reviewed; 226 AA.
AC O94318;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Vacuolar protein sorting-associated protein 20;
GN Name=vps20; ORFNames=SPBC215.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION.
RX PubMed=17660439; DOI=10.1099/mic.0.2007/006072-0;
RA Iwaki T., Onishi M., Ikeuchi M., Kita A., Sugiura R., Giga-Hama Y.,
RA Fukui Y., Takegawa K.;
RT "Essential roles of class E Vps proteins for sorting into multivesicular
RT bodies in Schizosaccharomyces pombe.";
RL Microbiology 153:2753-2764(2007).
CC -!- FUNCTION: Class E VPS protein implicated in concentration and sorting
CC of cargo proteins of the multivesicular body (MVB) for incorporation
CC into intralumenal vesicles. The lumenal sequestrated membrane proteins
CC will be targeted into the vacuole after fusion of the endosome with the
CC vacuole. {ECO:0000269|PubMed:17660439}.
CC -!- SUBUNIT: Component of the endosomal sorting required for transport
CC complex III (ESCRT-III). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}. Vacuole membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
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DR EMBL; CU329671; CAA22128.1; -; Genomic_DNA.
DR PIR; T39904; T39904.
DR RefSeq; NP_596691.1; NM_001022614.2.
DR AlphaFoldDB; O94318; -.
DR SMR; O94318; -.
DR BioGRID; 277248; 50.
DR IntAct; O94318; 1.
DR STRING; 4896.SPBC215.14c.1; -.
DR iPTMnet; O94318; -.
DR MaxQB; O94318; -.
DR PaxDb; O94318; -.
DR PRIDE; O94318; -.
DR EnsemblFungi; SPBC215.14c.1; SPBC215.14c.1:pep; SPBC215.14c.
DR GeneID; 2540725; -.
DR KEGG; spo:SPBC215.14c; -.
DR PomBase; SPBC215.14c; vps20.
DR VEuPathDB; FungiDB:SPBC215.14c; -.
DR eggNOG; KOG2910; Eukaryota.
DR HOGENOM; CLU_086201_0_1_1; -.
DR InParanoid; O94318; -.
DR OMA; GTIEFKL; -.
DR PhylomeDB; O94318; -.
DR Reactome; R-SPO-1632852; Macroautophagy.
DR Reactome; R-SPO-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR Reactome; R-SPO-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR PRO; PR:O94318; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005938; C:cell cortex; HDA:PomBase.
DR GO; GO:0000815; C:ESCRT III complex; ISO:PomBase.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045324; P:late endosome to vacuole transport; IMP:PomBase.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:PomBase.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR22761; PTHR22761; 1.
DR Pfam; PF03357; Snf7; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endosome; Lipoprotein; Membrane; Myristate; Phosphoprotein;
KW Protein transport; Reference proteome; Transport; Vacuole.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..226
FT /note="Vacuolar protein sorting-associated protein 20"
FT /id="PRO_0000362998"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 226 AA; 25556 MW; 4182EF95D872AAD3 CRC64;
MGVNSSKIND KDRSILSIKE QRDKLLRYSK RLEKIEQLEI DIARKCLRDS DKRGALRALK
AKKLYSGLIT QTYGQLGNIE QLLSTIEFTL IQKDVMFGLQ EGTNLIRQLQ ADMPLERVGR
ICNDRDEAMS YVDEVNDMLQ GRMSRDQEDE IQEELDSLIR EQEDEKVKDL EKPGFTPSTG
VDVLPSVPLK NAIPSLDESF PKAASVSNTS SAVVIDEELR KDPVLG
