VPS25_BOVIN
ID VPS25_BOVIN Reviewed; 176 AA.
AC Q5E9A6; Q3T0G0;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Vacuolar protein-sorting-associated protein 25;
DE AltName: Full=ESCRT-II complex subunit VPS25;
GN Name=VPS25;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex
CC required for transport II), which is required for multivesicular body
CC (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC MVB pathway mediates delivery of transmembrane proteins into the lumen
CC of the lysosome for degradation. The ESCRT-II complex is probably
CC involved in the recruitment of the ESCRT-III complex. The ESCRT-II
CC complex may also play a role in transcription regulation, possibly via
CC its interaction with ELL (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of a complex at least composed of ELL, SNF8/EAP30,
CC VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the ESCRT-II
CC complex (endosomal sorting complex required for transport II), composed
CC of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the
CC interaction requires misfolded CFTR. Interacts with the ESCRT-III
CC subunit CHMP6 (via N-terminal half) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome membrane
CC {ECO:0000250}. Nucleus {ECO:0000250}. Note=Distributes diffusely
CC throughout the cytoplasm and nucleoplasm, but exhibits a punctate
CC distribution on coexpression with CHMP6. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS25 family. {ECO:0000305}.
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DR EMBL; BT021014; AAX09031.1; -; mRNA.
DR EMBL; BC102410; AAI02411.1; -; mRNA.
DR RefSeq; NP_001015657.1; NM_001015657.2.
DR AlphaFoldDB; Q5E9A6; -.
DR SMR; Q5E9A6; -.
DR STRING; 9913.ENSBTAP00000026518; -.
DR PaxDb; Q5E9A6; -.
DR PRIDE; Q5E9A6; -.
DR Ensembl; ENSBTAT00000026518; ENSBTAP00000026518; ENSBTAG00000019906.
DR GeneID; 534750; -.
DR KEGG; bta:534750; -.
DR CTD; 84313; -.
DR VEuPathDB; HostDB:ENSBTAG00000019906; -.
DR VGNC; VGNC:36813; VPS25.
DR eggNOG; KOG4068; Eukaryota.
DR GeneTree; ENSGT00390000014892; -.
DR HOGENOM; CLU_087657_0_1_1; -.
DR InParanoid; Q5E9A6; -.
DR OMA; SQEFHGM; -.
DR OrthoDB; 1094121at2759; -.
DR TreeFam; TF317731; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000019906; Expressed in retina and 105 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0000814; C:ESCRT II complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; IBA:GO_Central.
DR GO; GO:0047485; F:protein N-terminus binding; IEA:Ensembl.
DR GO; GO:0005198; F:structural molecule activity; IBA:GO_Central.
DR GO; GO:0007175; P:negative regulation of epidermal growth factor-activated receptor activity; IEA:Ensembl.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.10.570; -; 1.
DR InterPro; IPR008570; ESCRT-II_cplx_Vps25-sub.
DR InterPro; IPR014041; ESCRT-II_cplx_Vps25-sub_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13149; PTHR13149; 1.
DR Pfam; PF05871; ESCRT-II; 1.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; Membrane; Nucleus; Protein transport;
KW Reference proteome; Transcription; Transcription regulation; Transport.
FT CHAIN 1..176
FT /note="Vacuolar protein-sorting-associated protein 25"
FT /id="PRO_0000215215"
SQ SEQUENCE 176 AA; 20747 MW; 3A82D09ADD9BF2BD CRC64;
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLTF CRLHKQSSMT VMEAQESPLF
NNVKLQRKLP VESIQVVLEE LRKKGNLEWL DKNKSSFLIM WRRPEEWGKL IYQWVSKSGQ
NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF
