ID   VPS25_DICDI             Reviewed;         194 AA.
AC   Q55GD9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Vacuolar protein-sorting-associated protein 25;
DE   AltName: Full=ESCRT-II complex subunit VPS25;
GN   Name=vps25; ORFNames=DDB_G0267708;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex
CC       required for transport II), which is required for multivesicular body
CC       (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC       MVB pathway mediates delivery of transmembrane proteins into the lumen
CC       of the lysosome for degradation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the endosomal sorting complex required for
CC       transport II (ESCRT-II). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the VPS25 family. {ECO:0000305}.
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DR   EMBL; AAFI02000003; EAL73307.1; -; Genomic_DNA.
DR   RefSeq; XP_647245.1; XM_642153.1.
DR   AlphaFoldDB; Q55GD9; -.
DR   SMR; Q55GD9; -.
DR   STRING; 44689.DDB0234026; -.
DR   PaxDb; Q55GD9; -.
DR   PRIDE; Q55GD9; -.
DR   EnsemblProtists; EAL73307; EAL73307; DDB_G0267708.
DR   GeneID; 8616050; -.
DR   KEGG; ddi:DDB_G0267708; -.
DR   dictyBase; DDB_G0267708; vps25.
DR   eggNOG; KOG4068; Eukaryota.
DR   HOGENOM; CLU_087657_0_1_1; -.
DR   InParanoid; Q55GD9; -.
DR   OMA; SQEFHGM; -.
DR   PhylomeDB; Q55GD9; -.
DR   Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   PRO; PR:Q55GD9; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0000814; C:ESCRT II complex; ISS:dictyBase.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0042803; F:protein homodimerization activity; IBA:GO_Central.
DR   GO; GO:0005198; F:structural molecule activity; IBA:GO_Central.
DR   GO; GO:0006605; P:protein targeting; ISS:dictyBase.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.10.570; -; 1.
DR   InterPro; IPR008570; ESCRT-II_cplx_Vps25-sub.
DR   InterPro; IPR014041; ESCRT-II_cplx_Vps25-sub_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR13149; PTHR13149; 1.
DR   Pfam; PF05871; ESCRT-II; 1.
DR   SUPFAM; SSF46785; SSF46785; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..194
FT                   /note="Vacuolar protein-sorting-associated protein 25"
FT                   /id="PRO_0000328263"
SQ   SEQUENCE   194 AA;  23306 MW;  EB7E036F814A720A CRC64;
     MSNQVFQFPP YYHKEPFFTI QPILNTRKKQ FQMWQDLILQ YCRYYKIYEL DINESIKSNS
     VLFNNEKINR KLSREALKSI IDDIIENGFA EWVDKEKEKE KEKDKDNNNN NRVLIMWRKP
     DEWASLIYKW VADCGLLNTV LTVWEIQNGD DSKKQEFHQL NTTILMKSLK VLEKQSKCQT
     FSQDENVGVK FFSI