VPS26_DROME
ID VPS26_DROME Reviewed; 478 AA.
AC Q9W552; O46088; Q4QQB4; Q95SD6;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Vacuolar protein sorting-associated protein 26;
DE AltName: Full=VPS26 protein homolog;
GN Name=Vps26; ORFNames=CG14804;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-478.
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP FUNCTION, PROBABLE COMPONENT OF THE RETROMER COMPLEX, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=18193032; DOI=10.1038/ncb1687;
RA Port F., Kuster M., Herr P., Furger E., Banziger C., Hausmann G.,
RA Basler K.;
RT "Wingless secretion promotes and requires retromer-dependent cycling of
RT Wntless.";
RL Nat. Cell Biol. 10:178-185(2008).
CC -!- FUNCTION: Component of the retromer complex which acts in conjunction
CC with wingless (wg) and clathrin-mediated endocytosis to sustain a
CC wntless (wls) traffic loop. This loop encompasses the Golgi, the cell
CC surface, an endocytic compartment and a retrograde route leading back
CC to the Golgi, thereby enabling wls to direct wg secretion. The hh and
CC dpp signaling pathways do not require the retromer complex suggesting
CC that it does not play a general role in exocytosis.
CC {ECO:0000269|PubMed:18193032}.
CC -!- SUBUNIT: Component of the retromer complex, composed of Vps26 and
CC Vps35. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18193032}. Membrane
CC {ECO:0000269|PubMed:18193032}; Peripheral membrane protein
CC {ECO:0000269|PubMed:18193032}.
CC -!- DISRUPTION PHENOTYPE: Accumulation of wg in the Golgi, preventing wg
CC from being secreted from the cells. {ECO:0000269|PubMed:18193032}.
CC -!- SIMILARITY: Belongs to the VPS26 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28390.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF45679.1; -; Genomic_DNA.
DR EMBL; AL021106; CAA15940.1; -; Genomic_DNA.
DR EMBL; BT023502; AAY84902.1; -; mRNA.
DR EMBL; AY060842; AAL28390.1; ALT_INIT; mRNA.
DR PIR; T12683; T12683.
DR RefSeq; NP_569952.2; NM_130596.3.
DR AlphaFoldDB; Q9W552; -.
DR SMR; Q9W552; -.
DR BioGRID; 57690; 10.
DR STRING; 7227.FBpp0070298; -.
DR PaxDb; Q9W552; -.
DR PRIDE; Q9W552; -.
DR DNASU; 31144; -.
DR EnsemblMetazoa; FBtr0070311; FBpp0070298; FBgn0014411.
DR GeneID; 31144; -.
DR KEGG; dme:Dmel_CG14804; -.
DR UCSC; CG14804-RA; d. melanogaster.
DR CTD; 31144; -.
DR FlyBase; FBgn0014411; Vps26.
DR VEuPathDB; VectorBase:FBgn0014411; -.
DR eggNOG; KOG3063; Eukaryota.
DR GeneTree; ENSGT00950000183064; -.
DR HOGENOM; CLU_029884_1_0_1; -.
DR InParanoid; Q9W552; -.
DR OMA; HQHVPLH; -.
DR OrthoDB; 987411at2759; -.
DR PhylomeDB; Q9W552; -.
DR Reactome; R-DME-3238698; WNT ligand biogenesis and trafficking.
DR BioGRID-ORCS; 31144; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Vps26; fly.
DR GenomeRNAi; 31144; -.
DR PRO; PR:Q9W552; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0014411; Expressed in embryonic/larval hemocyte (Drosophila) and 25 other tissues.
DR Genevisible; Q9W552; DM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; ISS:FlyBase.
DR GO; GO:0030904; C:retromer complex; IDA:UniProtKB.
DR GO; GO:0030906; C:retromer, cargo-selective complex; ISS:FlyBase.
DR GO; GO:0005112; F:Notch binding; IPI:FlyBase.
DR GO; GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
DR GO; GO:0048663; P:neuron fate commitment; IMP:FlyBase.
DR GO; GO:0030111; P:regulation of Wnt signaling pathway; IMP:UniProtKB.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:FlyBase.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.640; -; 2.
DR InterPro; IPR014752; Arrestin-like_C.
DR InterPro; IPR028934; Vps26-related.
DR PANTHER; PTHR12233; PTHR12233; 1.
DR Pfam; PF03643; Vps26; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Membrane; Protein transport; Reference proteome; Transport;
KW Wnt signaling pathway.
FT CHAIN 1..478
FT /note="Vacuolar protein sorting-associated protein 26"
FT /id="PRO_0000073011"
FT REGION 298..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 94
FT /note="R -> G (in Ref. 3; CAA15940)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 478 AA; 52656 MW; F9F9E108A8B60B26 CRC64;
MNFLGFGQSA DIEIVFDGAE HKTAEVKGED GKVEKMLLFY DGETVSGKVN VTLKKPGSKL
EHQGIKIEFI GQIELYYDRG NHHEFKCLAK ALARPGDLIQ NNSYPFDFPK VEKQFEVYAG
SNVRLRYFLR ATIVRRISDI TKEVDIAVHT LCSYPEMNNP IKMEVGIEDC LHIEFEYNKS
KYHLRDTIIG KIYFLLVRIK IKHMEIAIIK KESTGTGPTM FNENETIAKY EIMDGAPVKG
ESIPIRVFLA GYNLTPTMRD INKKFSVKYF LNLVLMDTED RRYFKQQEIT LWRKADKPRY
HGAQQHQQQQ HQHVPLHAPP HLVSGPAAPT VAHSLISSST DSGEVGGAPT APGTAGSESK
MGLFTRESPN QEFSQQQMDS PPLTPTPSTA SVAVAVPTAA SSVSEPAPER GIGDGAAAAT
TSASPVAMLS SSPPPLLPVS PLSRSETEAS EQVQPEDEDL DAITPNTKKT GATPLATD
