VPS27_MAGO7
ID VPS27_MAGO7 Reviewed; 713 AA.
AC A4QTV1; G4N3H2;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Vacuolar protein sorting-associated protein 27;
GN Name=VPS27; ORFNames=MGG_04958;
OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS fungus) (Pyricularia oryzae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX NCBI_TaxID=242507;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX PubMed=15846337; DOI=10.1038/nature03449;
RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL Nature 434:980-986(2005).
CC -!- FUNCTION: Component of the ESCRT-0 complex which is the sorting
CC receptor for ubiquitinated cargo proteins at the multivesicular body
CC (MVB) and recruits ESCRT-I to the MVB outer membrane. {ECO:0000250}.
CC -!- SUBUNIT: Component of the ESCRT-0 complex composed of HSE1 and VPS27.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- DOMAIN: The FYVE domain is involved in the binding to
CC phosphatidylinositol 3-phosphate (PtdIns(3)P) which is required for the
CC association to endosomal membranes.
CC -!- DOMAIN: Both IUM domains are necessary for efficient binding to
CC ubiquitin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS27 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001233; EHA52647.1; -; Genomic_DNA.
DR RefSeq; XP_003712454.1; XM_003712406.1.
DR AlphaFoldDB; A4QTV1; -.
DR SMR; A4QTV1; -.
DR STRING; 318829.MGG_04958T0; -.
DR PRIDE; A4QTV1; -.
DR EnsemblFungi; MGG_04958T0; MGG_04958T0; MGG_04958.
DR GeneID; 2675537; -.
DR KEGG; mgr:MGG_04958; -.
DR VEuPathDB; FungiDB:MGG_04958; -.
DR eggNOG; KOG1818; Eukaryota.
DR HOGENOM; CLU_011862_1_0_1; -.
DR InParanoid; A4QTV1; -.
DR OMA; PHSSCYS; -.
DR OrthoDB; 828765at2759; -.
DR Proteomes; UP000009058; Chromosome 3.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
DR GO; GO:0043130; F:ubiquitin binding; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IEA:UniProt.
DR GO; GO:0007034; P:vacuolar transport; IEA:UniProt.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProt.
DR Gene3D; 1.25.40.90; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR017073; HGS/VPS27.
DR InterPro; IPR003903; UIM_dom.
DR InterPro; IPR002014; VHS_dom.
DR InterPro; IPR000306; Znf_FYVE.
DR InterPro; IPR017455; Znf_FYVE-rel.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF01363; FYVE; 1.
DR Pfam; PF02809; UIM; 2.
DR Pfam; PF00790; VHS; 1.
DR PIRSF; PIRSF036956; Hrs_Vps27; 1.
DR SMART; SM00064; FYVE; 1.
DR SMART; SM00726; UIM; 2.
DR SMART; SM00288; VHS; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS50330; UIM; 1.
DR PROSITE; PS50179; VHS; 1.
DR PROSITE; PS50178; ZF_FYVE; 1.
PE 3: Inferred from homology;
KW Endosome; Membrane; Metal-binding; Reference proteome; Repeat; Zinc;
KW Zinc-finger.
FT CHAIN 1..713
FT /note="Vacuolar protein sorting-associated protein 27"
FT /id="PRO_0000292518"
FT DOMAIN 18..150
FT /note="VHS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00218"
FT DOMAIN 274..293
FT /note="UIM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT DOMAIN 324..343
FT /note="UIM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT ZN_FING 168..228
FT /note="FYVE-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT REGION 230..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 473..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 612..704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..267
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..317
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 495..547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 555..597
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 612..692
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 713 AA; 78060 MW; 927DAFA0410D255E CRC64;
MMSWWSSGAN TALDEQIEKA TSSSLEDIAL NLEISDVIRS KTVPPKDAMR SLKKRIGHKN
PNTQLSALEL TDTCVKNGGQ HFLVEIASRE FIDNLVSLLK ATGPAAVNAD VRARILGLIQ
SWAAVTQGRV ELSYIGEVYK TLQHEGFQFP PKVAVATSMI DSSAPPEWTD SDVCMRCRTP
FTFTNRKHHC RNCGSCFDQQ CSSKSIPLPH LGIMQPVRVD DGCYAKVTDK SRGSGGAGAG
AGYDRKSPSL YSSFPHKNRS SSAMQPRSAR VDDGFDEDLK KALAMSLEEV KSHSRNYAPA
SNGVANSGQS KVNGDSSATK TVEEEDDDLK AAIAASLADM EEQKKKHSAV LHEQTHSTEA
ASASTFVPPK NDYELTPVEA ENINLFATLV DRLQTQPPGT ILREPQIQEL YDSIGTLRPK
LARTYGETMS KHDTLLDLHA KLATVVRYYD RMLEERLSKA YGQRNFAGYN MHVPRQTSSP
YPSLQGPSAP ANVPGESFYT GQPQHDYAEP SRQPSYPPQA SGAQQQFHQY APPHQASQPS
EGWQPSHTPA PAAQYAGQPP QQSTESTHSH SSAHNHQNLA APSAPDQSLP TPTTDPAASY
YFNQQQVSAL QTPVSVPAEP VQSPYPNLQQ QPPAQYQHSP APQGQAQQQK PPQQPQQPYW
QHSASQNTAL PTNHQPWPQV PNRQSASYVQ EPLPSAPQHA PQKPVVEEAL IEL
