ID   VPS28_BOVIN             Reviewed;         221 AA.
AC   Q3T178;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Vacuolar protein sorting-associated protein 28 homolog;
DE   AltName: Full=ESCRT-I complex subunit VPS28;
GN   Name=VPS28;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC       trafficking process. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC       required for transport I) which consists of TSG101, VPS28, a VPS37
CC       protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC       Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of
CC       an ESCRT-I complex (endosomal sorting complex required for transport I)
CC       which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1
CC       stoichiometry. Interacts WITH VPS36; the interaction mediates the
CC       association with the ESCRT-II complex. Interacts with SNF8 and VPS25.
CC       Interacts with CEP55 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}. Late endosome
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the VPS28 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00642, ECO:0000255|PROSITE-ProRule:PRU00645}.
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DR   EMBL; BC102078; AAI02079.1; -; mRNA.
DR   RefSeq; NP_001030581.1; NM_001035504.2.
DR   AlphaFoldDB; Q3T178; -.
DR   BMRB; Q3T178; -.
DR   SMR; Q3T178; -.
DR   PRIDE; Q3T178; -.
DR   Ensembl; ENSBTAT00000054515; ENSBTAP00000049263; ENSBTAG00000026320.
DR   GeneID; 618785; -.
DR   KEGG; bta:618785; -.
DR   CTD; 51160; -.
DR   VEuPathDB; HostDB:ENSBTAG00000026320; -.
DR   VGNC; VGNC:54396; VPS28.
DR   GeneTree; ENSGT00390000007486; -.
DR   InParanoid; Q3T178; -.
DR   OMA; CDEFPTV; -.
DR   OrthoDB; 1281819at2759; -.
DR   Proteomes; UP000009136; Chromosome 14.
DR   Bgee; ENSBTAG00000026320; Expressed in vas deferens and 106 other tissues.
DR   ExpressionAtlas; Q3T178; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000813; C:ESCRT I complex; ISS:UniProtKB.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:UniProtKB.
DR   Gene3D; 1.20.120.1130; -; 1.
DR   Gene3D; 1.20.1440.200; -; 1.
DR   InterPro; IPR037202; ESCRT_assembly_dom.
DR   InterPro; IPR007143; Vps28.
DR   InterPro; IPR017899; VPS28_C.
DR   InterPro; IPR037206; VPS28_C_sf.
DR   InterPro; IPR017898; VPS28_N.
DR   InterPro; IPR038358; VPS28_N_sf.
DR   PANTHER; PTHR12937; PTHR12937; 1.
DR   Pfam; PF03997; VPS28; 1.
DR   PIRSF; PIRSF017535; VPS28; 1.
DR   SUPFAM; SSF140111; SSF140111; 1.
DR   SUPFAM; SSF140427; SSF140427; 1.
DR   PROSITE; PS51310; VPS28_C; 1.
DR   PROSITE; PS51313; VPS28_N; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cell membrane; Endosome; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..221
FT                   /note="Vacuolar protein sorting-associated protein 28
FT                   homolog"
FT                   /id="PRO_0000253019"
FT   DOMAIN          13..120
FT                   /note="VPS28 N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00645"
FT   DOMAIN          124..220
FT                   /note="VPS28 C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00642"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UK41"
SQ   SEQUENCE   221 AA;  25484 MW;  27F5F040963F734F CRC64;
     MFHGIPATPG MGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC
     VTPNEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD
     DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV
     SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A