VPS28_DICDI
ID VPS28_DICDI Reviewed; 288 AA.
AC Q54NF1;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Vacuolar protein sorting-associated protein 28;
DE AltName: Full=ESCRT-I complex subunit VPS28;
GN Name=vps28; ORFNames=DDB_G0285295;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC trafficking process. {ECO:0000250}.
CC -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC required for transport I). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC Late endosome membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS28 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00642, ECO:0000255|PROSITE-ProRule:PRU00645}.
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DR EMBL; AAFI02000077; EAL64811.1; -; Genomic_DNA.
DR RefSeq; XP_638318.1; XM_633226.1.
DR AlphaFoldDB; Q54NF1; -.
DR SMR; Q54NF1; -.
DR STRING; 44689.DDB0234023; -.
DR PaxDb; Q54NF1; -.
DR EnsemblProtists; EAL64811; EAL64811; DDB_G0285295.
DR GeneID; 8625036; -.
DR KEGG; ddi:DDB_G0285295; -.
DR dictyBase; DDB_G0285295; vps28.
DR eggNOG; KOG3284; Eukaryota.
DR HOGENOM; CLU_076417_1_0_1; -.
DR InParanoid; Q54NF1; -.
DR OMA; CDEFPTV; -.
DR PhylomeDB; Q54NF1; -.
DR Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR PRO; PR:Q54NF1; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0000813; C:ESCRT I complex; ISS:dictyBase.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0006605; P:protein targeting; ISS:dictyBase.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR Gene3D; 1.20.120.1130; -; 1.
DR Gene3D; 1.20.1440.200; -; 1.
DR InterPro; IPR037202; ESCRT_assembly_dom.
DR InterPro; IPR007143; Vps28.
DR InterPro; IPR017899; VPS28_C.
DR InterPro; IPR037206; VPS28_C_sf.
DR InterPro; IPR017898; VPS28_N.
DR InterPro; IPR038358; VPS28_N_sf.
DR PANTHER; PTHR12937; PTHR12937; 1.
DR Pfam; PF03997; VPS28; 1.
DR SUPFAM; SSF140111; SSF140111; 1.
DR SUPFAM; SSF140427; SSF140427; 1.
DR PROSITE; PS51310; VPS28_C; 1.
DR PROSITE; PS51313; VPS28_N; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endosome; Membrane; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..288
FT /note="Vacuolar protein sorting-associated protein 28"
FT /id="PRO_0000367435"
FT DOMAIN 74..181
FT /note="VPS28 N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00645"
FT DOMAIN 191..287
FT /note="VPS28 C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00642"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 288 AA; 32725 MW; 2EF80C8AD7C20579 CRC64;
MNINNSNNNF GEPPPPYSFN NSPLSSSPLG PPTLNNSNTN NNNNNNSTNN NNNNNNNNNN
NNSNKQFENM INLKSQNSFQ NIKEVKLFNN NIEREMYENL AELYSIIKVT EHLEKAYIRD
DVSPKDYTTA CSKLIAQFKS SQTLLKDQVS NVGQFMKDYD LNCKAAFDRL VIKGFPSTLE
HNTNESSTDS AMAKNVAEAV QLFITTMDSI RLKLVSVDGI YPLLSDLMES LNKNQWLGPT
FEGKEKIKNW ISILNQMKAT DELDDDQSRQ LLFDLDNSYN IFYKAIKS
