ID   VPS28_RAT               Reviewed;         228 AA.
AC   B5DEN9;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Vacuolar protein sorting-associated protein 28 homolog;
GN   Name=Vps28;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the endosomal sorting complex required for
CC       transport-I (ESCRT-I) complex, a regulator of vesicular trafficking
CC       process. The ESCRT-I complex participates in the sorting of membrane
CC       proteins into vesicles that bud into late endosomal compartments called
CC       multivesicular bodies, and is also involved in the budding of many
CC       viruses. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC       required for transport I) which consists of TSG101, VPS28, a VPS37
CC       protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC       Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of
CC       an ESCRT-I complex (endosomal sorting complex required for transport I)
CC       which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1
CC       stoichiometry. Interacts WITH VPS36; the interaction mediates the
CC       association with the ESCRT-II complex. Interacts with SNF8 and VPS25.
CC       Interacts with CEP55 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC       Cell membrane {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the VPS28 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00642, ECO:0000255|PROSITE-ProRule:PRU00645}.
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DR   EMBL; BC168742; AAI68742.1; -; mRNA.
DR   AlphaFoldDB; B5DEN9; -.
DR   BMRB; B5DEN9; -.
DR   SMR; B5DEN9; -.
DR   STRING; 10116.ENSRNOP00000019707; -.
DR   jPOST; B5DEN9; -.
DR   PaxDb; B5DEN9; -.
DR   PeptideAtlas; B5DEN9; -.
DR   PRIDE; B5DEN9; -.
DR   UCSC; RGD:1306537; rat.
DR   RGD; 1306537; Vps28.
DR   eggNOG; KOG3284; Eukaryota.
DR   HOGENOM; CLU_076417_2_0_1; -.
DR   InParanoid; B5DEN9; -.
DR   PhylomeDB; B5DEN9; -.
DR   TreeFam; TF313364; -.
DR   Reactome; R-RNO-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   PRO; PR:B5DEN9; -.
DR   Proteomes; UP000002494; Unplaced.
DR   Genevisible; B5DEN9; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR   GO; GO:0005769; C:early endosome; ISO:RGD.
DR   GO; GO:0005768; C:endosome; ISO:RGD.
DR   GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR   GO; GO:0000813; C:ESCRT I complex; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; ISO:RGD.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0045732; P:positive regulation of protein catabolic process; ISO:RGD.
DR   GO; GO:2000397; P:positive regulation of ubiquitin-dependent endocytosis; ISO:RGD.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:UniProtKB.
DR   Gene3D; 1.20.120.1130; -; 1.
DR   Gene3D; 1.20.1440.200; -; 1.
DR   InterPro; IPR037202; ESCRT_assembly_dom.
DR   InterPro; IPR007143; Vps28.
DR   InterPro; IPR017899; VPS28_C.
DR   InterPro; IPR037206; VPS28_C_sf.
DR   InterPro; IPR017898; VPS28_N.
DR   InterPro; IPR038358; VPS28_N_sf.
DR   PANTHER; PTHR12937; PTHR12937; 1.
DR   Pfam; PF03997; VPS28; 1.
DR   PIRSF; PIRSF017535; VPS28; 1.
DR   SUPFAM; SSF140111; SSF140111; 1.
DR   SUPFAM; SSF140427; SSF140427; 1.
DR   PROSITE; PS51310; VPS28_C; 1.
DR   PROSITE; PS51313; VPS28_N; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cell membrane; Cytoplasm; Endosome; Membrane;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..228
FT                   /note="Vacuolar protein sorting-associated protein 28
FT                   homolog"
FT                   /id="PRO_0000358932"
FT   DOMAIN          20..127
FT                   /note="VPS28 N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00645"
FT   DOMAIN          131..227
FT                   /note="VPS28 C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00642"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UK41"
SQ   SEQUENCE   228 AA;  26110 MW;  BB5A29EF96284643 CRC64;
     MFHGIPATPG VGALPCSVAA PGNKPELYEE VKLYKNARER EKYDNMAELF AVVKTMQALE
     KAYIKDCVTP NEYTAACSRL LVQYKAAFRQ VQGSEISSID EFCRKFRLDC PLAMERIKED
     RPITIKDDKG NLNRCIADVV SLFITVMDKL RLEIRAMDEI QPDLRELMET MHRMSHLPPD
     FEGRQTVSQW LQTLSGMSAS DELDDSQVRQ MLFDLESAYN AFNRFLHS