VPS2A_ARATH
ID VPS2A_ARATH Reviewed; 225 AA.
AC Q9SKI2; Q701A4; Q701B3; Q701B7; Q701C5; Q701E0; Q701E1; Q701E3; Q701F9;
AC Q701G8; Q701J8; Q8L915;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Vacuolar protein sorting-associated protein 2 homolog 1;
DE Short=AtVPS2-1;
DE AltName: Full=Charged multivesicular body protein 2 homolog 1;
DE AltName: Full=ESCRT-III complex subunit VPS2 homolog 1;
DE AltName: Full=SNF7-like protein;
GN Name=VPS2.1; Synonyms=CHMP2-1, SLP; OrderedLocusNames=At2g06530;
GN ORFNames=T12H3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 15-225.
RC STRAIN=cv. Ag-0, cv. Alc-0, cv. Bad, cv. Bas-1, cv. Bas-2, cv. Bas-3,
RC cv. Bij, cv. Bla-1, cv. Blh-1, cv. Br-0, cv. Bs-2, cv. Bur-0, cv. Can-0,
RC cv. Cha-1, cv. Cha-2, cv. Chi-0, cv. Chi-1, cv. Co, cv. Columbia, cv. Ct-1,
RC cv. Cvi-0, cv. Dar, cv. Di-1, cv. Dul, cv. Edi-0, cv. Es-0, cv. Est-0,
RC cv. Flo, cv. Gat, cv. Gie, cv. Gre-0, cv. Han, cv. HOG, cv. In-0, cv. Ita,
RC cv. K-oz-1, cv. K-oz-2, cv. K-oz-3, cv. Kaz-1, cv. Kaz-2, cv. Kaz-3,
RC cv. KEN, cv. Kent, cv. Kga, cv. Kly-1, cv. Kly-2, cv. Kly-3, cv. Kly-4,
RC cv. Kly-6, cv. Kn-0, cv. Ko-2, cv. Kol, cv. Kon, cv. La-0, cv. Leb-1,
RC cv. Leb-2, cv. Leb-3, cv. Leb-4, cv. Lim, cv. Lip-0, cv. Lis, cv. Ll-2,
RC cv. Mal, cv. Mas, cv. Mt-0, cv. Nos-1, cv. Nov-1, cv. Nov-2, cv. Nov-3,
RC cv. Oph, cv. Oy-0, cv. Pan-1, cv. Par, cv. Per-1, cv. Pog-0, cv. Rak-1,
RC cv. Rak-2, cv. Rak-3, cv. Rsch-0, cv. Rub, cv. Ryb-0, cv. Sah-0, cv. Sea-0,
RC cv. Sed, cv. Sev-1, cv. Sha, cv. Sie, cv. Sij-1, cv. Sij-2, cv. Sij-4,
RC cv. St-0, cv. Stw-0, cv. Tsar, cv. Tsu-0, cv. Wa-1, cv. Wassilewskija-1,
RC cv. Wil-1, and cv. Wt; TISSUE=Leaf;
RX PubMed=15045663; DOI=10.1055/s-2004-817837;
RA Schmuths H., Hoffmann M.H., Bachmann K.;
RT "Geographic distribution and recombination of genomic fragments on the
RT short arm of chromosome 2 of Arabidopsis thaliana.";
RL Plant Biol. 6:128-139(2004).
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=17090720; DOI=10.1242/dev.02654;
RA Spitzer C., Schellmann S., Sabovljevic A., Shahriari M., Keshavaiah C.,
RA Bechtold N., Herzog M., Mueller S., Hanisch F.-G., Huelskamp M.;
RT "The Arabidopsis elch mutant reveals functions of an ESCRT component in
RT cytokinesis.";
RL Development 133:4679-4689(2006).
RN [7]
RP IDENTIFICATION.
RX PubMed=16488176; DOI=10.1016/j.tplants.2006.01.008;
RA Winter V., Hauser M.-T.;
RT "Exploring the ESCRTing machinery in eukaryotes.";
RL Trends Plant Sci. 11:115-123(2006).
RN [8]
RP INTERACTION WITH SKD1.
RX PubMed=21810997; DOI=10.1105/tpc.111.087254;
RA Katsiarimpa A., Anzenberger F., Schlager N., Neubert S., Hauser M.T.,
RA Schwechheimer C., Isono E.;
RT "The Arabidopsis deubiquitinating enzyme AMSH3 interacts with ESCRT-III
RT subunits and regulates their localization.";
RL Plant Cell 23:3026-3040(2011).
RN [9]
RP INTERACTION WITH SKD1, AND REVIEW ON ESCRT-III.
RX PubMed=24812106; DOI=10.1104/pp.114.238378;
RA Cai Y., Zhuang X., Gao C., Wang X., Jiang L.;
RT "The Arabidopsis endosomal sorting complex required for transport III
RT regulates internal vesicle formation of the prevacuolar compartment and is
RT required for plant development.";
RL Plant Physiol. 165:1328-1343(2014).
CC -!- FUNCTION: Component of the ESCRT-III complex, which is required for
CC multivesicular bodies (MVBs) formation and sorting of endosomal cargo
CC proteins into MVBs. The ESCRT-III complex is probably involved in the
CC concentration of MVB cargo (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the endosomal sorting required for transport
CC complex III (ESCRT-III), composed at least of VPS2, VPS20, VPS24 and
CC VPS32 (By similarity). Interacts with SKD1 (PubMed:21810997,
CC PubMed:24812106). {ECO:0000250|UniProtKB:O43633,
CC ECO:0000269|PubMed:21810997, ECO:0000269|PubMed:24812106}.
CC -!- INTERACTION:
CC Q9SKI2; Q0WTY4: VPS2.2; NbExp=3; IntAct=EBI-3865345, EBI-3865323;
CC Q9SKI2; Q9FF81: VPS36; NbExp=3; IntAct=EBI-3865345, EBI-3865302;
CC Q9SKI2; Q9SCP9: VPS37-1; NbExp=3; IntAct=EBI-3865345, EBI-3865264;
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM67006.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC006420; AAD25152.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC06012.1; -; Genomic_DNA.
DR EMBL; AY090953; AAM13999.1; -; mRNA.
DR EMBL; AY088685; AAM67006.1; ALT_INIT; mRNA.
DR EMBL; AJ628449; CAF31658.1; -; Genomic_DNA.
DR EMBL; AJ628450; CAF31659.1; -; Genomic_DNA.
DR EMBL; AJ628451; CAF31660.1; -; Genomic_DNA.
DR EMBL; AJ628452; CAF31661.1; -; Genomic_DNA.
DR EMBL; AJ628453; CAF31662.1; -; Genomic_DNA.
DR EMBL; AJ628454; CAF31663.1; -; Genomic_DNA.
DR EMBL; AJ628455; CAF31664.1; -; Genomic_DNA.
DR EMBL; AJ628456; CAF31665.1; -; Genomic_DNA.
DR EMBL; AJ628457; CAF31666.1; -; Genomic_DNA.
DR EMBL; AJ628458; CAF31667.1; -; Genomic_DNA.
DR EMBL; AJ628459; CAF31668.1; -; Genomic_DNA.
DR EMBL; AJ628460; CAF31669.1; -; Genomic_DNA.
DR EMBL; AJ628461; CAF31670.1; -; Genomic_DNA.
DR EMBL; AJ628462; CAF31671.1; -; Genomic_DNA.
DR EMBL; AJ628463; CAF31672.1; -; Genomic_DNA.
DR EMBL; AJ628464; CAF31673.1; -; Genomic_DNA.
DR EMBL; AJ628465; CAF31674.1; -; Genomic_DNA.
DR EMBL; AJ628466; CAF31675.1; -; Genomic_DNA.
DR EMBL; AJ628467; CAF31676.1; -; Genomic_DNA.
DR EMBL; AJ628468; CAF31677.1; -; Genomic_DNA.
DR EMBL; AJ628469; CAF31678.1; -; Genomic_DNA.
DR EMBL; AJ628470; CAF31679.1; -; Genomic_DNA.
DR EMBL; AJ628471; CAF31680.1; -; Genomic_DNA.
DR EMBL; AJ628472; CAF31681.1; -; Genomic_DNA.
DR EMBL; AJ628473; CAF31682.1; -; Genomic_DNA.
DR EMBL; AJ628474; CAF31683.1; -; Genomic_DNA.
DR EMBL; AJ628475; CAF31684.1; -; Genomic_DNA.
DR EMBL; AJ628476; CAF31685.1; -; Genomic_DNA.
DR EMBL; AJ628477; CAF31686.1; -; Genomic_DNA.
DR EMBL; AJ628478; CAF31687.1; -; Genomic_DNA.
DR EMBL; AJ628479; CAF31688.1; -; Genomic_DNA.
DR EMBL; AJ628480; CAF31689.1; -; Genomic_DNA.
DR EMBL; AJ628481; CAF31690.1; -; Genomic_DNA.
DR EMBL; AJ628482; CAF31691.1; -; Genomic_DNA.
DR EMBL; AJ628483; CAF31692.1; -; Genomic_DNA.
DR EMBL; AJ628484; CAF31693.1; -; Genomic_DNA.
DR EMBL; AJ628485; CAF31694.1; -; Genomic_DNA.
DR EMBL; AJ628486; CAF31695.1; -; Genomic_DNA.
DR EMBL; AJ628487; CAF31696.1; -; Genomic_DNA.
DR EMBL; AJ628488; CAF31697.1; -; Genomic_DNA.
DR EMBL; AJ628489; CAF31698.1; -; Genomic_DNA.
DR EMBL; AJ628490; CAF31699.1; -; Genomic_DNA.
DR EMBL; AJ628491; CAF31700.1; -; Genomic_DNA.
DR EMBL; AJ628492; CAF31701.1; -; Genomic_DNA.
DR EMBL; AJ628493; CAF31702.1; -; Genomic_DNA.
DR EMBL; AJ628494; CAF31703.1; -; Genomic_DNA.
DR EMBL; AJ628495; CAF31704.1; -; Genomic_DNA.
DR EMBL; AJ628496; CAF31705.1; -; Genomic_DNA.
DR EMBL; AJ628497; CAF31706.1; -; Genomic_DNA.
DR EMBL; AJ628498; CAF31707.1; -; Genomic_DNA.
DR EMBL; AJ628499; CAF31708.1; -; Genomic_DNA.
DR EMBL; AJ628500; CAF31709.1; -; Genomic_DNA.
DR EMBL; AJ628501; CAF31710.1; -; Genomic_DNA.
DR EMBL; AJ628502; CAF31711.1; -; Genomic_DNA.
DR EMBL; AJ628503; CAF31712.1; -; Genomic_DNA.
DR EMBL; AJ628504; CAF31713.1; -; Genomic_DNA.
DR EMBL; AJ628505; CAF31714.1; -; Genomic_DNA.
DR EMBL; AJ628506; CAF31715.1; -; Genomic_DNA.
DR EMBL; AJ628507; CAF31716.1; -; Genomic_DNA.
DR EMBL; AJ628508; CAF31717.1; -; Genomic_DNA.
DR EMBL; AJ628509; CAF31718.1; -; Genomic_DNA.
DR EMBL; AJ628510; CAF31719.1; -; Genomic_DNA.
DR EMBL; AJ628511; CAF31720.1; -; Genomic_DNA.
DR EMBL; AJ628512; CAF31721.1; -; Genomic_DNA.
DR EMBL; AJ628513; CAF31722.1; -; Genomic_DNA.
DR EMBL; AJ628514; CAF31723.1; -; Genomic_DNA.
DR EMBL; AJ628515; CAF31724.1; -; Genomic_DNA.
DR EMBL; AJ628516; CAF31725.1; -; Genomic_DNA.
DR EMBL; AJ628517; CAF31726.1; -; Genomic_DNA.
DR EMBL; AJ628518; CAF31727.1; -; Genomic_DNA.
DR EMBL; AJ628519; CAF31728.1; -; Genomic_DNA.
DR EMBL; AJ628520; CAF31729.1; -; Genomic_DNA.
DR EMBL; AJ628521; CAF31730.1; -; Genomic_DNA.
DR EMBL; AJ628522; CAF31731.1; -; Genomic_DNA.
DR EMBL; AJ628523; CAF31732.1; -; Genomic_DNA.
DR EMBL; AJ628524; CAF31733.1; -; Genomic_DNA.
DR EMBL; AJ628525; CAF31734.1; -; Genomic_DNA.
DR EMBL; AJ628526; CAF31735.1; -; Genomic_DNA.
DR EMBL; AJ628527; CAF31736.1; -; Genomic_DNA.
DR EMBL; AJ628528; CAF31737.1; -; Genomic_DNA.
DR EMBL; AJ628529; CAF31738.1; -; Genomic_DNA.
DR EMBL; AJ628530; CAF31739.1; -; Genomic_DNA.
DR EMBL; AJ628531; CAF31740.1; -; Genomic_DNA.
DR EMBL; AJ628532; CAF31741.1; -; Genomic_DNA.
DR EMBL; AJ628533; CAF31742.1; -; Genomic_DNA.
DR EMBL; AJ628534; CAF31743.1; -; Genomic_DNA.
DR EMBL; AJ628535; CAF31744.1; -; Genomic_DNA.
DR EMBL; AJ628536; CAF31745.1; -; Genomic_DNA.
DR EMBL; AJ628537; CAF31746.1; -; Genomic_DNA.
DR EMBL; AJ628538; CAF31747.1; -; Genomic_DNA.
DR EMBL; AJ628539; CAF31748.1; -; Genomic_DNA.
DR EMBL; AJ628540; CAF31749.1; -; Genomic_DNA.
DR EMBL; AJ628541; CAF31750.1; -; Genomic_DNA.
DR EMBL; AJ628542; CAF31751.1; -; Genomic_DNA.
DR EMBL; AJ628543; CAF31752.1; -; Genomic_DNA.
DR EMBL; AJ628544; CAF31753.1; -; Genomic_DNA.
DR EMBL; AJ628545; CAF31754.1; -; Genomic_DNA.
DR EMBL; AJ628546; CAF31755.1; -; Genomic_DNA.
DR PIR; D84478; D84478.
DR RefSeq; NP_565336.1; NM_126650.4.
DR AlphaFoldDB; Q9SKI2; -.
DR SMR; Q9SKI2; -.
DR BioGRID; 606; 9.
DR IntAct; Q9SKI2; 6.
DR STRING; 3702.AT2G06530.1; -.
DR TCDB; 3.A.31.1.2; the endosomal sorting complexes required for transport iii (escrt-iii) family.
DR PaxDb; Q9SKI2; -.
DR PRIDE; Q9SKI2; -.
DR ProteomicsDB; 242568; -.
DR EnsemblPlants; AT2G06530.1; AT2G06530.1; AT2G06530.
DR GeneID; 815211; -.
DR Gramene; AT2G06530.1; AT2G06530.1; AT2G06530.
DR KEGG; ath:AT2G06530; -.
DR Araport; AT2G06530; -.
DR TAIR; locus:2054612; AT2G06530.
DR eggNOG; KOG3230; Eukaryota.
DR HOGENOM; CLU_069208_1_1_1; -.
DR InParanoid; Q9SKI2; -.
DR OMA; RYAKKFM; -.
DR OrthoDB; 1254120at2759; -.
DR PhylomeDB; Q9SKI2; -.
DR PRO; PR:Q9SKI2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKI2; baseline and differential.
DR Genevisible; Q9SKI2; AT.
DR GO; GO:0000815; C:ESCRT III complex; ISS:TAIR.
DR GO; GO:0005770; C:late endosome; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005771; C:multivesicular body; IDA:TAIR.
DR GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0070676; P:intralumenal vesicle formation; IDA:TAIR.
DR GO; GO:0045324; P:late endosome to vacuole transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IMP:TAIR.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR10476; PTHR10476; 1.
DR Pfam; PF03357; Snf7; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Protein transport; Reference proteome; Transport.
FT CHAIN 1..225
FT /note="Vacuolar protein sorting-associated protein 2
FT homolog 1"
FT /id="PRO_0000368195"
FT COILED 13..54
FT /evidence="ECO:0000255"
FT VARIANT 47
FT /note="E -> Q (in strain: cv. Ag-0)"
FT VARIANT 95
FT /note="K -> R (in strain: cv. Per-1)"
FT VARIANT 143
FT /note="V -> F (in strain: cv. Mal)"
FT VARIANT 182
FT /note="N -> S (in strain: cv. Bas-2)"
FT VARIANT 186
FT /note="G -> S (in strain: cv. Ct-1)"
FT VARIANT 192
FT /note="A -> T (in strain: cv. Tsar)"
FT VARIANT 193
FT /note="A -> T (in strain: cv. Edi-0, cv. Lip-0, cv. Sie and
FT cv. Bur-0)"
FT VARIANT 198
FT /note="V -> G (in strain: cv. Mas)"
FT VARIANT 198
FT /note="V -> I (in strain: cv. Dul)"
SQ SEQUENCE 225 AA; 25292 MW; 7068FB91553C1828 CRC64;
MMNSIFGKRK TPAELLRENK RMLDKSIREI ERERQGLQTQ EKKLINEIKK TAKQGQMGAV
KVMAKDLIRT RHQIEKFYKL KSQLQGVSLR IQTLKSTQAM GEAMKGVTKA MGQMNRQMNL
PSLQKIMQEF ERQNEKMEMV SEVMGDAIDD ALEGDEEEEE TEDLVSQVLD EIGIDINQEL
VNAPSGAVAV PAAKNKVVQA EATGAEDSGG IDSDLQARLD NLRKM
