VPS2B_ARATH
ID VPS2B_ARATH Reviewed; 222 AA.
AC Q0WTY4; B3H7E8; Q84TF3; Q9FGV7;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Vacuolar protein sorting-associated protein 2 homolog 2;
DE Short=AtVPS2-2;
DE AltName: Full=Charged multivesicular body protein 2 homolog 2;
DE AltName: Full=ESCRT-III complex subunit VPS2 homolog 2;
GN Name=VPS2.2; Synonyms=CHMP2-2; OrderedLocusNames=At5g44560;
GN ORFNames=MFC16.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-203.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=17090720; DOI=10.1242/dev.02654;
RA Spitzer C., Schellmann S., Sabovljevic A., Shahriari M., Keshavaiah C.,
RA Bechtold N., Herzog M., Mueller S., Hanisch F.-G., Huelskamp M.;
RT "The Arabidopsis elch mutant reveals functions of an ESCRT component in
RT cytokinesis.";
RL Development 133:4679-4689(2006).
RN [7]
RP IDENTIFICATION.
RX PubMed=16488176; DOI=10.1016/j.tplants.2006.01.008;
RA Winter V., Hauser M.-T.;
RT "Exploring the ESCRTing machinery in eukaryotes.";
RL Trends Plant Sci. 11:115-123(2006).
RN [8]
RP INTERACTION WITH CHMP1A; CHMP1B AND VPS60-1.
RX PubMed=22010978; DOI=10.1021/pr200845n;
RA Ibl V., Csaszar E., Schlager N., Neubert S., Spitzer C., Hauser M.T.;
RT "Interactome of the plant-specific ESCRT-III component AtVPS2.2 in
RT Arabidopsis thaliana.";
RL J. Proteome Res. 11:397-411(2012).
CC -!- FUNCTION: Component of the ESCRT-III complex, which is required for
CC multivesicular bodies (MVBs) formation and sorting of endosomal cargo
CC proteins into MVBs. The ESCRT-III complex is probably involved in the
CC concentration of MVB cargo (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the endosomal sorting required for transport
CC complex III (ESCRT-III), composed at least of VPS2, VPS20, VPS24 and
CC VPS32 (By similarity). Interacts with CHMP1A, CHMP1B and VPS60-1
CC (PubMed:22010978). {ECO:0000250|UniProtKB:O43633,
CC ECO:0000269|PubMed:22010978}.
CC -!- INTERACTION:
CC Q0WTY4; Q8LE58: CHMP1A; NbExp=4; IntAct=EBI-3865323, EBI-6391458;
CC Q0WTY4; Q9SKI2: VPS2.1; NbExp=3; IntAct=EBI-3865323, EBI-3865345;
CC Q0WTY4; O65421: VPS28-1; NbExp=4; IntAct=EBI-3865323, EBI-3865310;
CC Q0WTY4; Q9SZE4: VPS32.2; NbExp=3; IntAct=EBI-3865323, EBI-3865953;
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WTY4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WTY4-2; Sequence=VSP_036803;
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11575.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB024024; BAB11575.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB017065; BAB11575.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED95123.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95124.1; -; Genomic_DNA.
DR EMBL; AK227410; BAE99414.1; -; mRNA.
DR EMBL; BT005848; AAO64783.1; -; mRNA.
DR RefSeq; NP_001119371.1; NM_001125899.1. [Q0WTY4-2]
DR RefSeq; NP_199269.1; NM_123823.6. [Q0WTY4-1]
DR AlphaFoldDB; Q0WTY4; -.
DR SMR; Q0WTY4; -.
DR BioGRID; 19733; 39.
DR IntAct; Q0WTY4; 35.
DR STRING; 3702.AT5G44560.1; -.
DR TCDB; 3.A.31.1.2; the endosomal sorting complexes required for transport iii (escrt-iii) family.
DR PaxDb; Q0WTY4; -.
DR PRIDE; Q0WTY4; -.
DR ProteomicsDB; 242569; -. [Q0WTY4-1]
DR EnsemblPlants; AT5G44560.1; AT5G44560.1; AT5G44560. [Q0WTY4-1]
DR EnsemblPlants; AT5G44560.2; AT5G44560.2; AT5G44560. [Q0WTY4-2]
DR GeneID; 834483; -.
DR Gramene; AT5G44560.1; AT5G44560.1; AT5G44560. [Q0WTY4-1]
DR Gramene; AT5G44560.2; AT5G44560.2; AT5G44560. [Q0WTY4-2]
DR KEGG; ath:AT5G44560; -.
DR Araport; AT5G44560; -.
DR TAIR; locus:2163396; AT5G44560.
DR eggNOG; KOG3230; Eukaryota.
DR HOGENOM; CLU_069208_1_1_1; -.
DR InParanoid; Q0WTY4; -.
DR OMA; MEMTEDM; -.
DR OrthoDB; 1480977at2759; -.
DR PhylomeDB; Q0WTY4; -.
DR PRO; PR:Q0WTY4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0WTY4; baseline and differential.
DR Genevisible; Q0WTY4; AT.
DR GO; GO:0000815; C:ESCRT III complex; ISS:TAIR.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0045324; P:late endosome to vacuole transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR10476; PTHR10476; 1.
DR Pfam; PF03357; Snf7; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endosome; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..222
FT /note="Vacuolar protein sorting-associated protein 2
FT homolog 2"
FT /id="PRO_0000368196"
FT REGION 179..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 26..83
FT /evidence="ECO:0000255"
FT COILED 143..222
FT /evidence="ECO:0000255"
FT COMPBIAS 206..222
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..20
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036803"
FT CONFLICT 199
FT /note="A -> G (in Ref. 5; AAO64783)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="N -> S (in Ref. 2; BAE99414 and 5; AAO64783)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 222 AA; 24305 MW; C86FB65136DCE0AC CRC64;
MNIFKKKTTP KDALRTSKRE MAVATRGIER EITSLQLEEK RLVAEIKKTA KTGNEAATKI
LARQLVRLRQ QITNLQGSRA QIRGVTTHTQ ALYASTSISS GMKGATKAMV AMNKQMAPTK
QAKVIKDFQK QSAQLDMTIE MMSEAIDETL DKDEAEEETE DLTNQVLDEI GVGVASQLSS
APKGRIATKT AAPPASTAAT NKNSESSEVD ELEKRLASLR RI
