VPS33_ARATH
ID VPS33_ARATH Reviewed; 592 AA.
AC Q94KJ7; Q9SV35;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Vacuolar protein-sorting-associated protein 33 homolog;
DE Short=AtVPS33;
GN Name=VPS33; OrderedLocusNames=At3g54860; ORFNames=F28P10.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION IN THE C-VSP COMPLEX,
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=12589039; DOI=10.1091/mbc.e02-08-0509;
RA Rojo E., Zouhar J., Kovaleva V., Hong S., Raikhel N.V.;
RT "The AtC-VPS protein complex is localized to the tonoplast and the
RT prevacuolar compartment in arabidopsis.";
RL Mol. Biol. Cell 14:361-369(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [5]
RP SUBUNIT, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29463724; DOI=10.1073/pnas.1717839115;
RA Takemoto K., Ebine K., Askani J.C., Krueger F., Gonzalez Z.A., Ito E.,
RA Goh T., Schumacher K., Nakano A., Ueda T.;
RT "Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases
RT mediate membrane fusion at the vacuole in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E2457-E2466(2018).
CC -!- FUNCTION: Involved in regulating membrane fusion at the tonoplast and
CC the prevacuolar compartment. {ECO:0000269|PubMed:12589039}.
CC -!- SUBUNIT: Core component of at least two putative endosomal tethering
CC complexes, the homotypic fusion and vacuole protein sorting (HOPS)
CC complex and the class C core vacuole/endosome tethering (CORVET)
CC complex. Their common core is composed of the class C Vps proteins
CC VPS11, VCL1, VPS18 and VPS33, which in HOPS further associates with
CC VPS39 and VPS41 and in CORVET with VPS3. {ECO:0000269|PubMed:12589039,
CC ECO:0000269|PubMed:29463724}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:12589039};
CC Peripheral membrane protein {ECO:0000269|PubMed:12589039}. Prevacuolar
CC compartment membrane {ECO:0000269|PubMed:12589039}; Peripheral membrane
CC protein {ECO:0000269|PubMed:12589039}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q94KJ7-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, siliques and
CC flowers. {ECO:0000269|PubMed:12589039}.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB41098.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF357527; AAK48903.1; -; mRNA.
DR EMBL; AL049655; CAB41098.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79301.1; -; Genomic_DNA.
DR RefSeq; NP_567009.1; NM_115343.4. [Q94KJ7-1]
DR AlphaFoldDB; Q94KJ7; -.
DR SMR; Q94KJ7; -.
DR BioGRID; 9967; 2.
DR STRING; 3702.AT3G54860.2; -.
DR iPTMnet; Q94KJ7; -.
DR PaxDb; Q94KJ7; -.
DR PRIDE; Q94KJ7; -.
DR EnsemblPlants; AT3G54860.1; AT3G54860.1; AT3G54860. [Q94KJ7-1]
DR GeneID; 824651; -.
DR Gramene; AT3G54860.1; AT3G54860.1; AT3G54860. [Q94KJ7-1]
DR KEGG; ath:AT3G54860; -.
DR Araport; AT3G54860; -.
DR eggNOG; KOG1302; Eukaryota.
DR InParanoid; Q94KJ7; -.
DR OMA; FHIFFVP; -.
DR PhylomeDB; Q94KJ7; -.
DR PRO; PR:Q94KJ7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q94KJ7; baseline and differential.
DR Genevisible; Q94KJ7; AT.
DR GO; GO:0033263; C:CORVET complex; IDA:UniProtKB.
DR GO; GO:0030897; C:HOPS complex; IDA:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IPI:UniProtKB.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 1.25.40.850; -; 1.
DR Gene3D; 3.40.50.1910; -; 2.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR InterPro; IPR043155; VPS33_dom3b.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Reference proteome; Vacuole.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..592
FT /note="Vacuolar protein-sorting-associated protein 33
FT homolog"
FT /id="PRO_0000206309"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 592 AA; 66912 MW; 86D531598D1FEBA8 CRC64;
MAQIPSLENA PLNLKSIRDK SERELVNLLK DVRGTKCLVI DPKLSGSVSL IIPTSKLKEL
GLELRHLTAE PVQTECTKVV YLVRSQLSFM KFIASHIQND IAKAIQRDYY VYFVPRRSVA
CEKILEQEKV HNLVTVKEFP LYMVPLDEDV ISFELELSEK DCLVDGDVSS LWHIAKAIHE
LEFSFGVISK MRAKGKASVR VADILNRMQV EEPVNSNDVG RPEVDTLILL DREVDMVTPM
CSQLTYEGLI DEILHISNGA VEVDSSVMGA QQEGKKMKVP LNSSDKLFKE TRDLNFEVVV
QVLRQKAMTM KEDYTEINST QTVSELKDFV KKLNSLPEMT RHIHLAQHLT TFTSKQSFNS
QLDMEQTLVE AENYDICYEY IEEMIHKQEP LTNVLRLLVL FSVTNSGLPK KQFDYIRMEL
LHSYGFEHVV TLNNLEKAGL LKKQEFKSNW LTVKRTLKLI VEDTDTSRPN DIAYVYSGYA
PLSIRLIQQA IHSGWRPMED ILKLLPGPHL ETKRSGFPSS PSVDSLHGAS NGVADGRRSI
VLVVFIGGVT FAEISALRYL ASKEGMAYDL IVATTKIVNG ATLIETFMEK LG
