ID   VPS36_BOVIN             Reviewed;         386 AA.
AC   A5PK00;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Vacuolar protein-sorting-associated protein 36;
DE   AltName: Full=ESCRT-II complex subunit VPS36;
GN   Name=VPS36;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex
CC       required for transport II), which is required for multivesicular body
CC       (MVB) formation and sorting of endosomal cargo proteins into MVBs. The
CC       MVB pathway mediates delivery of transmembrane proteins into the lumen
CC       of the lysosome for degradation. The ESCRT-II complex is probably
CC       involved in the recruitment of the ESCRT-III complex. Its ability to
CC       bind ubiquitin probably plays a role in endosomal sorting of
CC       ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex
CC       may also play a role in transcription regulation, possibly via its
CC       interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
CC       {ECO:0000250|UniProtKB:Q86VN1, ECO:0000250|UniProtKB:Q91XD6}.
CC   -!- SUBUNIT: Component of a complex at least composed of ELL, SNF8/EAP30,
CC       VPS25/EAP20 and VPS36/EAP45. Component of the endosomal sorting complex
CC       required for transport II (ESCRT-II), composed of SNF8, VPS36 and two
CC       copies of VPS25. Interacts with VPS25, SNF8, TSG101 and CHMP6.
CC       Interacts (via GLUE domain) with ubiquitin. Interacts with RILPL1 (via
CC       the C-terminal domain); which recruits ESCRT-II to the endosome
CC       membranes. Interacts with ECPAS. {ECO:0000250|UniProtKB:P0C0A2,
CC       ECO:0000250|UniProtKB:Q86VN1, ECO:0000250|UniProtKB:Q91XD6}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC       Late endosome {ECO:0000250|UniProtKB:Q91XD6}. Membrane {ECO:0000250}.
CC       Nucleus {ECO:0000250}. Note=Colocalizes with ubiquitinated proteins on
CC       late endosomes. Recruited to the endosome membrane to participate in
CC       vesicle formation. {ECO:0000250|UniProtKB:Q91XD6}.
CC   -!- DOMAIN: The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates
CC       binding to ubiquitin and phosphoinosides.
CC       {ECO:0000250|UniProtKB:Q91XD6}.
CC   -!- SIMILARITY: Belongs to the VPS36 family. {ECO:0000305}.
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DR   EMBL; BC142301; AAI42302.1; -; mRNA.
DR   RefSeq; NP_001092409.1; NM_001098939.2.
DR   AlphaFoldDB; A5PK00; -.
DR   SMR; A5PK00; -.
DR   STRING; 9913.ENSBTAP00000005646; -.
DR   PaxDb; A5PK00; -.
DR   PRIDE; A5PK00; -.
DR   GeneID; 511223; -.
DR   KEGG; bta:511223; -.
DR   CTD; 51028; -.
DR   eggNOG; KOG2760; Eukaryota.
DR   InParanoid; A5PK00; -.
DR   OrthoDB; 981902at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0000814; C:ESCRT II complex; IBA:GO_Central.
DR   GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:InterPro.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR021648; GLUE_dom.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR040608; Snf8/Vps36.
DR   InterPro; IPR037855; Vps36.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR13128; PTHR13128; 1.
DR   Pfam; PF04157; EAP30; 1.
DR   Pfam; PF11605; Vps36_ESCRT-II; 1.
DR   SUPFAM; SSF46785; SSF46785; 2.
DR   PROSITE; PS51495; GLUE; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; Endosome; Lipid-binding; Membrane; Nucleus;
KW   Protein transport; Reference proteome; Transcription;
KW   Transcription regulation; Transport.
FT   CHAIN           1..386
FT                   /note="Vacuolar protein-sorting-associated protein 36"
FT                   /id="PRO_0000383119"
FT   DOMAIN          1..88
FT                   /note="GLUE N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00828"
FT   DOMAIN          105..138
FT                   /note="GLUE C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00828"
FT   COILED          160..185
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   386 AA;  43791 MW;  E0B2FD58AA56DD57 CRC64;
     MDRFVWTSGL LEINETLVIQ QRGVRIYDGE EKIKFDPGTL LLSTHRLIWR DQKNHECCMA
     IPLSQIVFIE EQAAGIGKSA KIVVHLHPAA SNKEPGPFQS SKNSYIKLSF KEHGQIEFYR
     RLSEEMTQRR WENMPVPQSL QTNRGPQPGR IRAVGIVGIE RKLEEKRKET DKNISEAFED
     LSKLMVKAKE MVELSKSIAN KIKDKQGDIT EDETIRFKSY LLSMGIANPV TRETYGSGTQ
     YHMQLAKQLA GILQAPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP
     LRLRVFDSGV MVIELQSHKE EEMVASALET VSEKGSLTSE EFAKLMGMSV LLAKERLLLA
     EKMGHLCRDD SVEGLRFYPN LFMTQS