VPS41_ARATH
ID VPS41_ARATH Reviewed; 980 AA.
AC P93043; Q9SGE1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 3.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Vacuolar protein sorting-associated protein 41 homolog;
GN Name=VPS41; OrderedLocusNames=At1g08190; ORFNames=T23G18.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 648-980.
RX PubMed=9159129; DOI=10.1073/pnas.94.11.5662;
RA Radisky D.C., Snyder W.B., Emr S.D., Kaplan J.;
RT "Characterization of VPS41, a gene required for vacuolar trafficking and
RT high-affinity iron transport in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:5662-5666(1997).
RN [4]
RP SUBUNIT, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29463724; DOI=10.1073/pnas.1717839115;
RA Takemoto K., Ebine K., Askani J.C., Krueger F., Gonzalez Z.A., Ito E.,
RA Goh T., Schumacher K., Nakano A., Ueda T.;
RT "Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases
RT mediate membrane fusion at the vacuole in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E2457-E2466(2018).
CC -!- FUNCTION: Required for vacuolar assembly and vacuolar traffic.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the homotypic fusion and vacuole protein sorting
CC (HOPS) complex composed of the class C Vps core proteins VPS11, VCL1,
CC VPS18 and VPS33, which in HOPS further associates with VPS39 and VPS41.
CC {ECO:0000269|PubMed:29463724}.
CC -!- SIMILARITY: Belongs to the VPS41 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF18239.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011438; AAF18239.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28259.1; -; Genomic_DNA.
DR EMBL; U86663; AAB60858.1; -; Genomic_DNA.
DR RefSeq; NP_172297.2; NM_100693.4.
DR AlphaFoldDB; P93043; -.
DR SMR; P93043; -.
DR STRING; 3702.AT1G08190.1; -.
DR iPTMnet; P93043; -.
DR PaxDb; P93043; -.
DR PRIDE; P93043; -.
DR ProteomicsDB; 242748; -.
DR EnsemblPlants; AT1G08190.1; AT1G08190.1; AT1G08190.
DR GeneID; 837340; -.
DR Gramene; AT1G08190.1; AT1G08190.1; AT1G08190.
DR KEGG; ath:AT1G08190; -.
DR Araport; AT1G08190; -.
DR TAIR; locus:2200008; AT1G08190.
DR eggNOG; KOG2066; Eukaryota.
DR HOGENOM; CLU_001285_2_2_1; -.
DR InParanoid; P93043; -.
DR OMA; KFKYQRI; -.
DR OrthoDB; 722805at2759; -.
DR PhylomeDB; P93043; -.
DR PRO; PR:P93043; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P93043; baseline and differential.
DR Genevisible; P93043; AT.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0030897; C:HOPS complex; IDA:UniProtKB.
DR GO; GO:0005770; C:late endosome; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; IBA:GO_Central.
DR GO; GO:0034058; P:endosomal vesicle fusion; IBA:GO_Central.
DR GO; GO:0009630; P:gravitropism; IMP:TAIR.
DR GO; GO:0016236; P:macroautophagy; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR000547; Clathrin_H-chain/VPS_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR016902; VPS41.
DR InterPro; IPR045111; Vps41/Vps8.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR12616; PTHR12616; 1.
DR Pfam; PF00637; Clathrin; 1.
DR PIRSF; PIRSF028921; VPS41; 1.
DR SMART; SM00299; CLH; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50236; CHCR; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
PE 1: Evidence at protein level;
KW Protein transport; Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..980
FT /note="Vacuolar protein sorting-associated protein 41
FT homolog"
FT /id="PRO_0000212825"
FT REPEAT 57..95
FT /note="WD 1"
FT REPEAT 96..135
FT /note="WD 2"
FT REPEAT 185..221
FT /note="WD 3"
FT REPEAT 613..769
FT /note="CHCR"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 931..964
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..38
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 940..957
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 648
FT /note="R -> G (in Ref. 3; AAB60858)"
FT /evidence="ECO:0000305"
FT CONFLICT 948
FT /note="E -> EE (in Ref. 3; AAB60858)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 980 AA; 110279 MW; E9A41687FC84FB58 CRC64;
MAAVPPENGV DGDDEREEEE EDEEEEEEEE EEENGDEAEE EPRLKYQRMG GNVPALLSND
AASCIAVAAR MIALGTHDGT VRILDLLGNQ VKEFRAHTAP VNDINFDTEG EYIGSCSDDG
SVVINSLFTD DEKMKFDYHR PMKAISLDPD YTKKQSKRFV AGGLAGHLYM NSKKWFGNKD
QVLHSGEGPI HSVKWRGSLI AWANDVGVKV YDTAKDQRVT FIEKPRGSPR PEALLPHLVW
QDDTLLVIGW GTSVKIASIK SDQQQTGTFR QIQMSSLTQV DIVASFQTSY YISGIAPFGD
SLVILAYIPI EGDGEKEFSS TTTLSRQGNA QRPEIRIVSW NNDELTMDAL PVHGFEHYKA
KDYSLAHAPF PGSSYAGGQW AAGDEPLYYI VSPKDVVIAK PRDAEDHINW LLQHGFHEKA
LAAVEASEGR TELIDKVGAG YLDHLIVERK YAEAASLCPK LLRGSASAWE RWVFHFAQLR
QLPVLVPYMP TDNPRLKDTV YEVALVALAT NPSYHKELLS AVKSWPRSVY SALTVISAIE
PQLNTSSMTD ALKEALAELY VIDGQYQKAF SLYADLLKPE VFDFIEKYSL HEAIRGKVVQ
LMLLDCKRAT VLFIQNRDLI PPSEVVPQLL KAGKNPQVLK AGKKCDSRYY LYLYLHALFE
VSHDTGKDFH DMQVELYAEY DTKMLLPFLR SSQHYKLEKA YELCVKKDFL REQVFVLGRM
GNAKQALAVI INKLGDIEEA VEFVSMQHDD DLWEELIKQC LNKPEMVGLL LEHTVGNLDP
LYIVNMVPNG LEIPRLRDRL VKIVTDYRTE TSLRHGCNDI LKTDIVNLLV KCFNEARRGV
CLSHEDDDSR AKREDNNRSS FSQRMVVDKS LSIKMTEVKS KTRGDTRCCM CFDPVSIRGD
TVVVFFCCHA YHETCLMDAA FSNNNHKTTK GSSGYEYSYD NGVDEEEEDE EEDEDGDGDR
PGRSRLRCIL CTTAAAASAR
