ID   VPS45_DICDI             Reviewed;         563 AA.
AC   Q54GE3;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Vacuolar protein sorting-associated protein 45;
GN   Name=vps45; ORFNames=DDB_G0290213;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: May play a role in vesicle-mediated protein trafficking from
CC       the Golgi stack through the trans-Golgi network. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Endosome membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC       Note=Associated with Golgi/endosomal vesicles and the trans-Golgi
CC       network. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR   EMBL; AAFI02000161; EAL62328.1; -; Genomic_DNA.
DR   RefSeq; XP_635835.1; XM_630743.1.
DR   AlphaFoldDB; Q54GE3; -.
DR   SMR; Q54GE3; -.
DR   STRING; 44689.DDB0234175; -.
DR   PaxDb; Q54GE3; -.
DR   EnsemblProtists; EAL62328; EAL62328; DDB_G0290213.
DR   GeneID; 8627543; -.
DR   KEGG; ddi:DDB_G0290213; -.
DR   dictyBase; DDB_G0290213; vps45.
DR   eggNOG; KOG1299; Eukaryota.
DR   HOGENOM; CLU_013933_3_1_1; -.
DR   InParanoid; Q54GE3; -.
DR   OMA; VHQLNNA; -.
DR   PhylomeDB; Q54GE3; -.
DR   Reactome; R-DDI-6811438; Intra-Golgi traffic.
DR   PRO; PR:Q54GE3; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; ISS:dictyBase.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0051082; F:unfolded protein binding; ISS:dictyBase.
DR   GO; GO:0006896; P:Golgi to vacuole transport; ISS:dictyBase.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   Gene3D; 3.40.50.1910; -; 1.
DR   Gene3D; 3.40.50.2060; -; 1.
DR   Gene3D; 3.90.830.10; -; 1.
DR   InterPro; IPR043154; Sec-1-like_dom1.
DR   InterPro; IPR043127; Sec-1-like_dom3a.
DR   InterPro; IPR001619; Sec1-like.
DR   InterPro; IPR027482; Sec1-like_dom2.
DR   InterPro; IPR036045; Sec1-like_sf.
DR   PANTHER; PTHR11679; PTHR11679; 1.
DR   Pfam; PF00995; Sec1; 1.
DR   PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR   SUPFAM; SSF56815; SSF56815; 1.
PE   1: Evidence at protein level;
KW   Endosome; Golgi apparatus; Membrane; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..563
FT                   /note="Vacuolar protein sorting-associated protein 45"
FT                   /id="PRO_0000328593"
SQ   SEQUENCE   563 AA;  64570 MW;  EF5A6084490A34DF CRC64;
     MSGKQMDVIA SIQEYINKIL TNIQGMKVLV LDKETAGIVS MVYTQSEILQ KEVFLFEKIE
     NTKEKMLHMK GVYFIRPTQE NIQSICDELK DPKFNKYHLF FTNTISKVSL DEIAKADEQD
     VVSEIQEYFG DFFAVNPDTF TLNLPGMLTK KSPRWQGDVG RVVDGLFSSL LALKKKPVIR
     YSSNSDTTRY LAEKITERMN RDRDLFDFRR QGEPLLLILD RKDDPITPLL HQWTYQAMIH
     ELLTINNNRV SLAKAPGIKD ELKEVVLSLD HDIFYKENLY KNFGDLGASI KDLVDQFQDK
     MNTNQNIQTI DDMKKFIENY PNFQKFSTTV SKHVSLMDEL NRLISLDNLM EVSEVQQELA
     CNHDHNSIYN HVLEIVNDSK YTDRDKLVLV LLYSIRYEDG RVWELKEKLS SIGIPPKEIG
     LIDTLRGYAG ASLREGDLLG TKNIFSFARS VVKRGLQGVS NIYTQHKPLL HDILDSILKN
     KLKETSYPYL STTQSRERPQ DVIIFMVGGI TYEEALTVYT FNSLNTGVCR VVLGGTSILN
     REQFLEDLSS TQISNPSSSS GRR