VPS45_HUMAN
ID VPS45_HUMAN Reviewed; 570 AA.
AC Q9NRW7; D3DUZ9; F5H8K1; Q15715; Q53FR8; Q5T4P6; Q9Y4Z6;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Vacuolar protein sorting-associated protein 45;
DE Short=h-VPS45;
DE Short=hlVps45;
GN Name=VPS45; Synonyms=VPS45A, VPS45B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8996080; DOI=10.1016/s0378-1119(96)00367-8;
RA Pevsner J., Hsu S.-C., Hyde P.S., Scheller R.H.;
RT "Mammalian homologues of yeast vacuolar protein sorting (vps) genes
RT implicated in Golgi-to-lysosome trafficking.";
RL Gene 183:7-14(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Leukocyte;
RX PubMed=10404641; DOI=10.1016/s1357-2725(99)00017-5;
RA Rajasekariah P., Eyre H.J., Stanley K.K., Walls R.S., Sutherland G.R.;
RT "Molecular cloning and characterization of a cDNA encoding the human
RT leucocyte vacuolar protein sorting (hlVps45).";
RL Int. J. Biochem. Cell Biol. 31:683-694(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hematopoietic stem cell;
RA Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C.,
RA Ren S., Tu Y., Chen Z.;
RT "Novel genes expressed in hematopoietic stem/progenitor cells from
RT myelodysplastic syndrome patients.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Gastric mucosa;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Colon, and Hippocampus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP INTERACTION WITH ZFYVE20.
RX PubMed=11062261; DOI=10.1083/jcb.151.3.601;
RA Nielsen E., Christoforidis S., Uttenweiler-Joseph S., Miaczynska M.,
RA Dewitte F., Wilm M., Hoflack B., Zerial M.;
RT "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited
RT to endosomes through a FYVE finger domain.";
RL J. Cell Biol. 151:601-612(2000).
RN [10]
RP INTERACTION WITH ZFYVE20.
RX PubMed=11788822; DOI=10.1038/ncb744;
RA de Renzis S., Soennichsen B., Zerial M.;
RT "Divalent Rab effectors regulate the sub-compartmental organization and
RT sorting of early endosomes.";
RL Nat. Cell Biol. 4:124-133(2002).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [14]
RP VARIANTS SCN5 ASN-224 AND LYS-238.
RX PubMed=23738510; DOI=10.1056/nejmoa1301296;
RA Vilboux T., Lev A., Malicdan M.C., Simon A.J., Jarvinen P., Racek T.,
RA Puchalka J., Sood R., Carrington B., Bishop K., Mullikin J., Huizing M.,
RA Garty B.Z., Eyal E., Wolach B., Gavrieli R., Toren A., Soudack M.,
RA Atawneh O.M., Babushkin T., Schiby G., Cullinane A., Avivi C.,
RA Polak-Charcon S., Barshack I., Amariglio N., Rechavi G.,
RA van der Werff ten Bosch J., Anikster Y., Klein C., Gahl W.A., Somech R.;
RT "A congenital neutrophil defect syndrome associated with mutations in
RT VPS45.";
RL N. Engl. J. Med. 369:54-65(2013).
CC -!- FUNCTION: May play a role in vesicle-mediated protein trafficking from
CC the Golgi stack through the trans-Golgi network.
CC -!- SUBUNIT: Interacts with STX6 (By similarity). Interacts with ZFYVE20.
CC {ECO:0000250, ECO:0000269|PubMed:11062261,
CC ECO:0000269|PubMed:11788822}.
CC -!- INTERACTION:
CC Q9NRW7; Q9H1K0: RBSN; NbExp=4; IntAct=EBI-1782543, EBI-1105310;
CC Q9NRW7; O14662-5: STX16; NbExp=3; IntAct=EBI-1782543, EBI-9089968;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Note=Associated with Golgi/endosomal vesicles and the trans-Golgi
CC network. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NRW7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NRW7-2; Sequence=VSP_056739, VSP_056740, VSP_056741;
CC -!- TISSUE SPECIFICITY: Ubiquitous. Expression was highest in testis, heart
CC and brain, intermediate in kidney, spleen, prostate, ovary, small
CC intestine and thymus and low in lung, skeletal muscle, placenta, colon,
CC pancreas, peripheral blood leukocytes and liver.
CC -!- DISEASE: Neutropenia, severe congenital 5, autosomal recessive (SCN5)
CC [MIM:615285]: An autosomal recessive primary immunodeficiency disorder
CC characterized primarily by neutropenia and neutrophil dysfunction, a
CC lack of response to G-CSF, life-threatening infections, bone marrow
CC fibrosis, and renal extramedullary hematopoiesis.
CC {ECO:0000269|PubMed:23738510}. Note=The disease is caused by variants
CC affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR EMBL; U35246; AAC50931.1; -; mRNA.
DR EMBL; AJ133421; CAB40417.1; -; mRNA.
DR EMBL; AF165513; AAF86643.1; -; mRNA.
DR EMBL; AK023170; BAB14443.1; -; mRNA.
DR EMBL; AK223214; BAD96934.1; -; mRNA.
DR EMBL; AL358073; CAI14265.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53584.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53585.1; -; Genomic_DNA.
DR EMBL; BC012932; AAH12932.1; -; mRNA.
DR EMBL; BC028382; AAH28382.1; -; mRNA.
DR CCDS; CCDS60244.1; -. [Q9NRW7-2]
DR CCDS; CCDS944.1; -. [Q9NRW7-1]
DR PIR; JC5722; JC5722.
DR RefSeq; NP_001266282.1; NM_001279353.1. [Q9NRW7-2]
DR RefSeq; NP_001266283.1; NM_001279354.1.
DR RefSeq; NP_009190.2; NM_007259.4. [Q9NRW7-1]
DR AlphaFoldDB; Q9NRW7; -.
DR SMR; Q9NRW7; -.
DR BioGRID; 116443; 150.
DR DIP; DIP-46823N; -.
DR IntAct; Q9NRW7; 36.
DR MINT; Q9NRW7; -.
DR STRING; 9606.ENSP00000358126; -.
DR iPTMnet; Q9NRW7; -.
DR PhosphoSitePlus; Q9NRW7; -.
DR BioMuta; VPS45; -.
DR DMDM; 23396937; -.
DR EPD; Q9NRW7; -.
DR jPOST; Q9NRW7; -.
DR MassIVE; Q9NRW7; -.
DR MaxQB; Q9NRW7; -.
DR PaxDb; Q9NRW7; -.
DR PeptideAtlas; Q9NRW7; -.
DR PRIDE; Q9NRW7; -.
DR ProteomicsDB; 27808; -.
DR ProteomicsDB; 82431; -. [Q9NRW7-1]
DR Antibodypedia; 34017; 302 antibodies from 31 providers.
DR DNASU; 11311; -.
DR Ensembl; ENST00000369128.9; ENSP00000358124.5; ENSG00000136631.15. [Q9NRW7-2]
DR Ensembl; ENST00000535106.5; ENSP00000440690.2; ENSG00000136631.15. [Q9NRW7-2]
DR Ensembl; ENST00000644510.2; ENSP00000495563.1; ENSG00000136631.15. [Q9NRW7-1]
DR GeneID; 11311; -.
DR KEGG; hsa:11311; -.
DR MANE-Select; ENST00000644510.2; ENSP00000495563.1; NM_007259.5; NP_009190.2.
DR UCSC; uc001etp.5; human. [Q9NRW7-1]
DR CTD; 11311; -.
DR DisGeNET; 11311; -.
DR GeneCards; VPS45; -.
DR HGNC; HGNC:14579; VPS45.
DR HPA; ENSG00000136631; Low tissue specificity.
DR MalaCards; VPS45; -.
DR MIM; 610035; gene.
DR MIM; 615285; phenotype.
DR neXtProt; NX_Q9NRW7; -.
DR OpenTargets; ENSG00000136631; -.
DR Orphanet; 369852; Congenital neutropenia-myelofibrosis-nephromegaly syndrome.
DR PharmGKB; PA37901; -.
DR VEuPathDB; HostDB:ENSG00000136631; -.
DR eggNOG; KOG1299; Eukaryota.
DR GeneTree; ENSGT00550000075028; -.
DR HOGENOM; CLU_013933_3_1_1; -.
DR InParanoid; Q9NRW7; -.
DR OMA; VHQLNNA; -.
DR OrthoDB; 651641at2759; -.
DR PhylomeDB; Q9NRW7; -.
DR TreeFam; TF300407; -.
DR PathwayCommons; Q9NRW7; -.
DR Reactome; R-HSA-6811438; Intra-Golgi traffic.
DR Reactome; R-HSA-9754560; SARS-CoV-2 modulates autophagy.
DR Reactome; R-HSA-983231; Factors involved in megakaryocyte development and platelet production.
DR SignaLink; Q9NRW7; -.
DR BioGRID-ORCS; 11311; 192 hits in 1091 CRISPR screens.
DR ChiTaRS; VPS45; human.
DR GeneWiki; VPS45; -.
DR GenomeRNAi; 11311; -.
DR Pharos; Q9NRW7; Tbio.
DR PRO; PR:Q9NRW7; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9NRW7; protein.
DR Bgee; ENSG00000136631; Expressed in cerebellar cortex and 207 other tissues.
DR ExpressionAtlas; Q9NRW7; baseline and differential.
DR Genevisible; Q9NRW7; HS.
DR GO; GO:0010008; C:endosome membrane; TAS:Reactome.
DR GO; GO:0005794; C:Golgi apparatus; ISS:BHF-UCL.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISS:BHF-UCL.
DR GO; GO:0008021; C:synaptic vesicle; IEA:Ensembl.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 1.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disease variant; Endosome; Golgi apparatus; Membrane;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..570
FT /note="Vacuolar protein sorting-associated protein 45"
FT /id="PRO_0000206312"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT MOD_RES 441
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O08700"
FT VAR_SEQ 1..36
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_056739"
FT VAR_SEQ 124..192
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_056740"
FT VAR_SEQ 542..570
FT /note="SFLEEVLASGLHSRSKESSQVTSRSASRR -> RDGVSLCSPAWFRTPGLKR
FT STRLSLPKCWDYSFPRGSSGFWTAQPKQGELSSHIKVSEQKMKRWLGEGHSFLSCPHYR
FT FSLLNKGVGEQLWVLCWLLELISR (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_056741"
FT VARIANT 224
FT /note="T -> N (in SCN5; patient fibroblasts are
FT characterized by impaired motility and increased apoptosis;
FT dbSNP:rs879255237)"
FT /evidence="ECO:0000269|PubMed:23738510"
FT /id="VAR_069865"
FT VARIANT 238
FT /note="E -> K (in SCN5; dbSNP:rs782269909)"
FT /evidence="ECO:0000269|PubMed:23738510"
FT /id="VAR_069866"
FT CONFLICT 81..82
FT /note="YI -> SL (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="T -> S (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 163
FT /note="T -> A (in Ref. 5; BAD96934)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="A -> G (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="T -> S (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="C -> L (in Ref. 2; CAB40417)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="G -> D (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 298
FT /note="K -> R (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 370
FT /note="I -> V (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="A -> V (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 407..408
FT /note="MM -> IV (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 412
FT /note="N -> H (in Ref. 5; BAD96934)"
FT /evidence="ECO:0000305"
FT CONFLICT 412
FT /note="N -> S (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 416
FT /note="S -> A (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="R -> K (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 537
FT /note="V -> I (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 557
FT /note="K -> R (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
FT CONFLICT 562
FT /note="V -> A (in Ref. 1; AAC50931)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 65077 MW; 5574F0BE3A7D4EB3 CRC64;
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR
EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA
EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS
SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL
GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK
EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ
NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR
KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG
RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT
KSFLEEVLAS GLHSRSKESS QVTSRSASRR
