VPS45_MOUSE
ID VPS45_MOUSE Reviewed; 570 AA.
AC P97390; Q91VK9;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Vacuolar protein sorting-associated protein 45;
DE Short=mVps45;
GN Name=Vps45; Synonyms=Vps45a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH STX6.
RC STRAIN=Swiss albino; TISSUE=Adipocyte;
RX PubMed=9045632; DOI=10.1074/jbc.272.10.6187;
RA Tellam J.T., James D.E., Stevens T.H., Piper R.C.;
RT "Identification of a mammalian Golgi Sec1p-like protein, mVps45.";
RL J. Biol. Chem. 272:6187-6193(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in vesicle-mediated protein trafficking from
CC the Golgi stack through the trans-Golgi network.
CC -!- SUBUNIT: Interacts with ZFYVE20 (By similarity). Interacts with STX6.
CC {ECO:0000250, ECO:0000269|PubMed:9045632}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane
CC protein. Endosome membrane; Peripheral membrane protein.
CC Note=Associated with Golgi/endosomal vesicles and the trans-Golgi
CC network.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR EMBL; U66865; AAB37577.1; -; mRNA.
DR EMBL; BC012691; AAH12691.1; -; mRNA.
DR EMBL; BC058528; AAH58528.1; -; mRNA.
DR CCDS; CCDS17628.1; -.
DR RefSeq; NP_038869.1; NM_013841.3.
DR AlphaFoldDB; P97390; -.
DR SMR; P97390; -.
DR BioGRID; 204535; 8.
DR IntAct; P97390; 2.
DR MINT; P97390; -.
DR STRING; 10090.ENSMUSP00000015891; -.
DR iPTMnet; P97390; -.
DR PhosphoSitePlus; P97390; -.
DR SwissPalm; P97390; -.
DR EPD; P97390; -.
DR jPOST; P97390; -.
DR MaxQB; P97390; -.
DR PaxDb; P97390; -.
DR PeptideAtlas; P97390; -.
DR PRIDE; P97390; -.
DR ProteomicsDB; 275183; -.
DR Antibodypedia; 34017; 302 antibodies from 31 providers.
DR DNASU; 22365; -.
DR Ensembl; ENSMUST00000015891; ENSMUSP00000015891; ENSMUSG00000015747.
DR GeneID; 22365; -.
DR KEGG; mmu:22365; -.
DR UCSC; uc008qly.2; mouse.
DR CTD; 11311; -.
DR MGI; MGI:891965; Vps45.
DR VEuPathDB; HostDB:ENSMUSG00000015747; -.
DR eggNOG; KOG1299; Eukaryota.
DR GeneTree; ENSGT00550000075028; -.
DR HOGENOM; CLU_013933_3_1_1; -.
DR InParanoid; P97390; -.
DR OMA; VHQLNNA; -.
DR OrthoDB; 651641at2759; -.
DR PhylomeDB; P97390; -.
DR TreeFam; TF300407; -.
DR Reactome; R-MMU-6811438; Intra-Golgi traffic.
DR Reactome; R-MMU-983231; Factors involved in megakaryocyte development and platelet production.
DR BioGRID-ORCS; 22365; 15 hits in 74 CRISPR screens.
DR ChiTaRS; Vps45; mouse.
DR PRO; PR:P97390; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; P97390; protein.
DR Bgee; ENSMUSG00000015747; Expressed in retinal neural layer and 233 other tissues.
DR Genevisible; P97390; MM.
DR GO; GO:0010008; C:endosome membrane; IDA:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0000139; C:Golgi membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0008021; C:synaptic vesicle; ISO:MGI.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 1.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..570
FT /note="Vacuolar protein sorting-associated protein 45"
FT /id="PRO_0000206313"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NRW7"
FT MOD_RES 441
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O08700"
FT CONFLICT 55
FT /note="I -> M (in Ref. 2; AAH12691)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 65053 MW; DA704FDE8469F488 CRC64;
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR
EIMKHLKAIC FLRPTKENVE YLIQELRRPK YSIYFIYFSN VISKSDVKSL AEADEQEVVA
EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS
SEAAKRLGEC VKQVISKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL
GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKRPK
EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ
NDHSSALQNV KRLLQNPKVT EFDAVRLVML YALHYERHSS NSLPGLIVDL RSKGVAEKYR
KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG
RLKENLYPYL GPSTLRDRPQ DIIVFIIGGA TYEEALTVYN LNRTTPGVRI VLGGTTIHNT
KSFLEEVLAS GLHSRSRESS QATSRSANRR
