VPS45_RAT
ID VPS45_RAT Reviewed; 570 AA.
AC O08700;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Vacuolar protein sorting-associated protein 45;
DE Short=rVps45;
GN Name=Vps45; Synonyms=Vps45a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=9106478; DOI=10.1016/s0005-2736(97)00014-x;
RA El-Husseini A.E.-D., Guthrie H., Snutch T.P., Vincent S.R.;
RT "Molecular cloning of a mammalian homologue of the yeast vesicular
RT transport protein vps45.";
RL Biochim. Biophys. Acta 1325:8-12(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: May play a role in vesicle-mediated protein trafficking from
CC the Golgi stack through the trans-Golgi network.
CC -!- SUBUNIT: Interacts with STX6 and ZFYVE20. {ECO:0000250}.
CC -!- INTERACTION:
CC O08700; Q8BVI5: Stx16; Xeno; NbExp=6; IntAct=EBI-7849203, EBI-3871057;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Endosome membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Note=Associated with Golgi/endosomal vesicles and the trans-Golgi
CC network. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in
CC brain. Detected in every part of the brain.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR EMBL; U81160; AAB53041.1; -; mRNA.
DR EMBL; BC081705; AAH81705.1; -; mRNA.
DR RefSeq; NP_742069.1; NM_172072.2.
DR AlphaFoldDB; O08700; -.
DR SMR; O08700; -.
DR BioGRID; 249101; 2.
DR IntAct; O08700; 3.
DR MINT; O08700; -.
DR STRING; 10116.ENSRNOP00000028751; -.
DR iPTMnet; O08700; -.
DR PhosphoSitePlus; O08700; -.
DR jPOST; O08700; -.
DR PaxDb; O08700; -.
DR PRIDE; O08700; -.
DR Ensembl; ENSRNOT00000028751; ENSRNOP00000028751; ENSRNOG00000021173.
DR GeneID; 64516; -.
DR KEGG; rno:64516; -.
DR UCSC; RGD:621267; rat.
DR CTD; 11311; -.
DR RGD; 621267; Vps45.
DR eggNOG; KOG1299; Eukaryota.
DR GeneTree; ENSGT00550000075028; -.
DR HOGENOM; CLU_013933_3_1_1; -.
DR InParanoid; O08700; -.
DR OMA; VHQLNNA; -.
DR OrthoDB; 651641at2759; -.
DR PhylomeDB; O08700; -.
DR TreeFam; TF300407; -.
DR Reactome; R-RNO-6811438; Intra-Golgi traffic.
DR Reactome; R-RNO-983231; Factors involved in megakaryocyte development and platelet production.
DR PRO; PR:O08700; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000021173; Expressed in cerebellum and 20 other tissues.
DR Genevisible; O08700; RN.
DR GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
DR GO; GO:0000139; C:Golgi membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IDA:BHF-UCL.
DR GO; GO:0008021; C:synaptic vesicle; IDA:SynGO.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 1.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..570
FT /note="Vacuolar protein sorting-associated protein 45"
FT /id="PRO_0000206314"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NRW7"
FT MOD_RES 441
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 570 AA; 64894 MW; 0FF5E2D8E7E7E571 CRC64;
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR
EIMKHLKAIC FLRPTKENVD SLIQELRRPK YSIYFIYFSN VISKSDVKSL AEADEQEVVA
EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS
SEAAKRLGEC VKQVISKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL
GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKRPK
EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ
NDHSSALQNV KRLLQNPKVT EFDAVRLVML YALHYERHSS NSLPGLIVDL RSKGVAEKYR
KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG
KLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTIHNT
KSFLEEVLAS GLHSRSRESS QATSRSASRR
