VPS45_SCHPO
ID VPS45_SCHPO Reviewed; 558 AA.
AC Q09805;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Vacuolar protein sorting-associated protein 45;
GN Name=vps45; ORFNames=SPAC2G11.03c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17287531; DOI=10.1534/genetics.107.070946;
RA Miyatake M., Kuno T., Kita A., Katsura K., Takegawa K., Uno S., Nabata T.,
RA Sugiura R.;
RT "Valproic acid affects membrane trafficking and cell-wall integrity in
RT fission yeast.";
RL Genetics 175:1695-1705(2007).
CC -!- FUNCTION: Vacuolar protein sorting-associated protein involved in
CC Golgi-to-vacuole protein transport. {ECO:0000269|PubMed:17287531}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}. Vacuole membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: displays an accumulation of abnormal vesicular
CC structures and leads to valproic acid sensitivity.
CC {ECO:0000269|PubMed:17287531}.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
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DR EMBL; CU329670; CAA91168.1; -; Genomic_DNA.
DR PIR; S62458; S62458.
DR RefSeq; NP_593083.1; NM_001018481.2.
DR AlphaFoldDB; Q09805; -.
DR SMR; Q09805; -.
DR BioGRID; 277987; 1.
DR STRING; 4896.SPAC2G11.03c.1; -.
DR MaxQB; Q09805; -.
DR PaxDb; Q09805; -.
DR EnsemblFungi; SPAC2G11.03c.1; SPAC2G11.03c.1:pep; SPAC2G11.03c.
DR GeneID; 2541485; -.
DR KEGG; spo:SPAC2G11.03c; -.
DR PomBase; SPAC2G11.03c; vps45.
DR VEuPathDB; FungiDB:SPAC2G11.03c; -.
DR eggNOG; KOG1299; Eukaryota.
DR HOGENOM; CLU_013933_3_1_1; -.
DR InParanoid; Q09805; -.
DR OMA; VHQLNNA; -.
DR PhylomeDB; Q09805; -.
DR PRO; PR:Q09805; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005768; C:endosome; IDA:PomBase.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000149; F:SNARE binding; ISM:PomBase.
DR GO; GO:0048210; P:Golgi vesicle fusion to target membrane; ISO:PomBase.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 1.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Membrane; Nucleus; Protein transport; Reference proteome;
KW Transport; Vacuole.
FT CHAIN 1..558
FT /note="Vacuolar protein sorting-associated protein 45"
FT /id="PRO_0000206317"
SQ SEQUENCE 558 AA; 63552 MW; 2C138B7CDF5CFEA1 CRC64;
MDLVSASQSY FKRIFQEVSD LKILLLEEDT TKIVSSCITQ SNLLEQQIYL TVLLGNKREK
LRHLKCVAFL RPTPTTLRLL CEELRDPKYA EYHLYFTNVI PKSFLERLAE SDDFEAVKSI
QEFFLDYLVV NNDLASFNIP HIIEDSPDNW QDGAFHRTHQ GIISLLLSLK KKPVIRYDNN
SLLCLKLAEE VSYTIQHESQ LFNFRKPDTA PILLLLDRKN DPITPLLTQW TYQAMVHELF
GIDNGRVSFS NSTSDNEKST EIVLNPTLDP FYKETRFDNF GDLGVKIKDY VSHLQTKSTK
KASEIESIAD MKQFLEAYPE YRRLSGNVSK HVSLVSEISQ VVQRENLLEV GEVEQSLVCN
EPQSTDFNDI QRLLFSNISE NTKLRLAALY SLRFERIDPA KVSALQQMLI AGGVNPLKVS
VIPTLLHVAG YSFRQGDVFP PSNLFSRARS GLKGLRGVEN VYIQHNPFLK SILLDLIQGR
LKETTHPFLN SETRAQTSNE KPQDIIVVIV GGATYEEAHF VSEFNATQPG VRIILAGTTI
LNSTAYIDDI MYMSTRIK
