VPS51_YEAST
ID VPS51_YEAST Reviewed; 164 AA.
AC P36116; D6VX85;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Vacuolar protein sorting-associated protein 51;
DE AltName: Full=Apical bud growth protein 3;
GN Name=VPS51; Synonyms=API3, VPS67, WHI6; OrderedLocusNames=YKR020W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11957109; DOI=10.1007/s10142-001-0043-1;
RA Bidlingmaier S., Snyder M.A.;
RT "Large-scale identification of genes important for apical growth in
RT Saccharomyces cerevisiae by directed allele replacement technology (DART)
RT screening.";
RL Funct. Integr. Genomics 1:345-356(2002).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12377769; DOI=10.1074/jbc.m209428200;
RA Siniossoglou S., Pelham H.R.B.;
RT "Vps51p links the VFT complex to the SNARE Tlg1p.";
RL J. Biol. Chem. 277:48318-48324(2002).
RN [6]
RP FUNCTION.
RX PubMed=12134085; DOI=10.1091/mbc.02-01-0005;
RA Bonangelino C.J., Chavez E.M., Bonifacino J.S.;
RT "Genomic screen for vacuolar protein sorting genes in Saccharomyces
RT cerevisiae.";
RL Mol. Biol. Cell 13:2486-2501(2002).
RN [7]
RP FUNCTION, AND INTERACTION WITH VPS52; VPS53; VPS54 AND TGL1.
RX PubMed=12446664; DOI=10.1074/jbc.m210436200;
RA Reggiori F., Wang C.-W., Stromhaug P.E., Shintani T., Klionsky D.J.;
RT "Vps51 is part of the yeast Vps fifty-three tethering complex essential for
RT retrograde traffic from the early endosome and Cvt vesicle completion.";
RL J. Biol. Chem. 278:5009-5020(2003).
RN [8]
RP FUNCTION, AND INTERACTION WITH VPS52; VPS53 AND VPS54.
RX PubMed=12686613; DOI=10.1091/mbc.e02-10-0654;
RA Conibear E., Cleck J.N., Stevens T.H.;
RT "Vps51p mediates the association of the GARP (Vps52/53/54) complex with the
RT late Golgi t-SNARE Tlg1p.";
RL Mol. Biol. Cell 14:1610-1623(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in retrograde transport from early and late
CC endosomes to late Golgi. Links the Golgi-associated retrograde protein
CC (GARP) complex to the t-SNARE TLG1, leading to the fusion between the
CC endosomal vesicles with the late Golgi. May also be indirectly involved
CC in apical bud growth. {ECO:0000269|PubMed:11957109,
CC ECO:0000269|PubMed:12134085, ECO:0000269|PubMed:12377769,
CC ECO:0000269|PubMed:12446664, ECO:0000269|PubMed:12686613}.
CC -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC complex, also called VPS fifty three (VFT) complex, composed of VPS51,
CC VPS52, VPS53 and VPS54. Interacts with the t-SNARE TLG1.
CC {ECO:0000269|PubMed:12446664, ECO:0000269|PubMed:12686613}.
CC -!- INTERACTION:
CC P36116; Q03322: TLG1; NbExp=6; IntAct=EBI-26352, EBI-38705;
CC P36116; P39904: VPS52; NbExp=5; IntAct=EBI-26352, EBI-16418;
CC P36116; Q12071: VPS54; NbExp=3; IntAct=EBI-26352, EBI-36751;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000269|PubMed:12377769}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12377769}. Endosome membrane
CC {ECO:0000269|PubMed:12377769}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12377769}.
CC -!- MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the VPS51 family. {ECO:0000305}.
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DR EMBL; Z28245; CAA82092.1; -; Genomic_DNA.
DR EMBL; AY558556; AAS56882.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09175.1; -; Genomic_DNA.
DR PIR; S38089; S38089.
DR RefSeq; NP_012945.1; NM_001179810.1.
DR PDB; 2C5I; X-ray; 2.30 A; P=2-30.
DR PDB; 2C5K; X-ray; 2.05 A; P=9-32.
DR PDBsum; 2C5I; -.
DR PDBsum; 2C5K; -.
DR AlphaFoldDB; P36116; -.
DR SMR; P36116; -.
DR BioGRID; 34152; 545.
DR ComplexPortal; CPX-1718; GARP complex.
DR DIP; DIP-2096N; -.
DR IntAct; P36116; 5.
DR MINT; P36116; -.
DR STRING; 4932.YKR020W; -.
DR iPTMnet; P36116; -.
DR MaxQB; P36116; -.
DR PaxDb; P36116; -.
DR PRIDE; P36116; -.
DR EnsemblFungi; YKR020W_mRNA; YKR020W; YKR020W.
DR GeneID; 853890; -.
DR KEGG; sce:YKR020W; -.
DR SGD; S000001728; VPS51.
DR VEuPathDB; FungiDB:YKR020W; -.
DR eggNOG; ENOG502S3JK; Eukaryota.
DR HOGENOM; CLU_141194_0_0_1; -.
DR InParanoid; P36116; -.
DR OMA; MTEQISH; -.
DR BioCyc; YEAST:G3O-31996-MON; -.
DR EvolutionaryTrace; P36116; -.
DR PRO; PR:P36116; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36116; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000938; C:GARP complex; IPI:SGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:SGD.
DR GO; GO:0007118; P:budding cell apical bud growth; IDA:SGD.
DR GO; GO:0090156; P:cellular sphingolipid homeostasis; IMP:SGD.
DR GO; GO:0016239; P:positive regulation of macroautophagy; IMP:SGD.
DR GO; GO:0006623; P:protein targeting to vacuole; IDA:ComplexPortal.
DR GO; GO:0008361; P:regulation of cell size; HMP:SGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IDA:SGD.
DR GO; GO:0016050; P:vesicle organization; IMP:SGD.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Coiled coil; Endosome; Golgi apparatus;
KW Membrane; Protein transport; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..164
FT /note="Vacuolar protein sorting-associated protein 51"
FT /id="PRO_0000065905"
FT REGION 30..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 73..164
FT /evidence="ECO:0000255"
FT COMPBIAS 30..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT HELIX 16..29
FT /evidence="ECO:0007829|PDB:2C5K"
SQ SEQUENCE 164 AA; 18912 MW; 7E76264F654AC452 CRC64;
MAEQISHKKS LRVSSLNKDR RLLLREFYNL ENEPNKGRQE ARIGEKASEA HSGEEQVTDV
NIDTEANTEK PVKDDELSAT EEDLKEGSED AEEEIKNLPF KRLVQIHNKL LGKETETNNS
IKNTIYENYY DLIKVNDLLK EITNANEDQI NKLKQTVESL IKEL
