VPS52_CAEEL
ID VPS52_CAEEL Reviewed; 702 AA.
AC G5EFV8; G5EFV9;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2013, sequence version 2.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Vacuolar protein sorting-associated protein 52 homolog {ECO:0000250|UniProtKB:O01839, ECO:0000303|PubMed:21613545};
GN Name=vps-52 {ECO:0000312|WormBase:F08C6.3};
GN Synonyms=tag-197 {ECO:0000312|WormBase:F08C6.3}; ORFNames=F08C6.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ADO17797.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION IN THE GARP COMPLEX,
RP INTERACTION WITH VPS-53; VPS-54; RAB-6.1 AND RAB-6.2, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=21613545; DOI=10.1091/mbc.e10-06-0493;
RA Luo L., Hannemann M., Koenig S., Hegermann J., Ailion M., Cho M.K.,
RA Sasidharan N., Zweckstetter M., Rensing S.A., Eimer S.;
RT "The Caenorhabditis elegans GARP complex contains the conserved Vps51
RT subunit and is required to maintain lysosomal morphology.";
RL Mol. Biol. Cell 22:2564-2578(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=27191843; DOI=10.1371/journal.pgen.1006074;
RA Topalidou I., Cattin-Ortola J., Pappas A.L., Cooper K., Merrihew G.E.,
RA MacCoss M.J., Ailion M.;
RT "The EARP complex and its interactor eipr-1 are required for cargo sorting
RT to dense-core vesicles.";
RL PLoS Genet. 12:E1006074-E1006074(2016).
CC -!- FUNCTION: Acts as component of the GARP complex that is involved in
CC retrograde transport from early and late endosomes to the trans-Golgi
CC network (TGN) (PubMed:21613545). The GARP complex facilitates tethering
CC as well as SNARE complex assembly at the Golgi (PubMed:21613545). Plays
CC a role in the trafficking of cargo to dense-core vesicles, probably
CC through association with the EARP-interacting protein eipr-1
CC (PubMed:27191843). Important for neuronal function (PubMed:27191843).
CC {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:27191843}.
CC -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC complex, also called VFT (VPS fifty-three) complex, composed of vps-51,
CC vps-52, vps-53 and vps-54. Within the complex interacts with vps-53 and
CC vps-54 (PubMed:21613545). Interacts with the small GTPases rab-6.1 and
CC rab-6.2 (PubMed:21613545). {ECO:0000269|PubMed:21613545}.
CC -!- INTERACTION:
CC G5EFV8; O01839: vps-51; NbExp=6; IntAct=EBI-318981, EBI-313277;
CC G5EFV8; P34561: vps-53; NbExp=3; IntAct=EBI-318981, EBI-6394890;
CC G5EFV8; Q22639: vps-54; NbExp=5; IntAct=EBI-318981, EBI-6395200;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000269|PubMed:21613545}. Perikaryon
CC {ECO:0000269|PubMed:27191843}. Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:27191843}. Note=Co-localizes with rab-2 to
CC perinuclear puncta in the perikaryon. Co-localizes with the small
CC GTPases rab-6.1 and rab-6.2 at Golgi structures (PubMed:21613545).
CC {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:27191843}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed, with particularly strong
CC expression in neuronal cells (PubMed:21613545). Specifically expressed
CC in head and tail neurons and in the pharynx and ventral cord motor
CC neurons (PubMed:27191843). {ECO:0000269|PubMed:21613545,
CC ECO:0000269|PubMed:27191843}.
CC -!- DISRUPTION PHENOTYPE: Enlarged lysosomes (PubMed:21613545). Reduced
CC brood size (PubMed:21613545). Egg-laying defect, slow, but coordinated
CC locomotion, and reduced levels of unprocessed and processed cargo in
CC the motor neuron axon of the dorsal nerve cord (PubMed:27191843).
CC {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:27191843}.
CC -!- SIMILARITY: Belongs to the VPS52 family. {ECO:0000255}.
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DR EMBL; HQ237455; ADO17797.1; -; mRNA.
DR EMBL; BX284606; CCD67346.2; -; Genomic_DNA.
DR RefSeq; NP_509282.4; NM_076881.6.
DR AlphaFoldDB; G5EFV8; -.
DR SMR; G5EFV8; -.
DR BioGRID; 45942; 19.
DR ComplexPortal; CPX-365; GARP complex.
DR IntAct; G5EFV8; 18.
DR STRING; 6239.F08C6.3; -.
DR EPD; G5EFV8; -.
DR PaxDb; G5EFV8; -.
DR PeptideAtlas; G5EFV8; -.
DR EnsemblMetazoa; F08C6.3.1; F08C6.3.1; WBGene00007059.
DR GeneID; 181018; -.
DR KEGG; cel:CELE_F08C6.3; -.
DR CTD; 181018; -.
DR WormBase; F08C6.3; CE47824; WBGene00007059; vps-52.
DR eggNOG; KOG1961; Eukaryota.
DR GeneTree; ENSGT00390000008815; -.
DR HOGENOM; CLU_010797_0_0_1; -.
DR InParanoid; G5EFV8; -.
DR OMA; IHVVMVE; -.
DR OrthoDB; 158568at2759; -.
DR PhylomeDB; G5EFV8; -.
DR SignaLink; G5EFV8; -.
DR PRO; PR:G5EFV8; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00007059; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0000938; C:GARP complex; IPI:WormBase.
DR GO; GO:0005797; C:Golgi medial cisterna; IDA:WormBase.
DR GO; GO:0000138; C:Golgi trans cisterna; IDA:WormBase.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; IPI:WormBase.
DR GO; GO:0019905; F:syntaxin binding; IPI:WormBase.
DR GO; GO:0032456; P:endocytic recycling; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0007041; P:lysosomal transport; IBA:GO_Central.
DR GO; GO:1904810; P:negative regulation of dense core granule transport; IMP:UniProtKB.
DR GO; GO:1904811; P:positive regulation of dense core granule transport; IMP:UniProtKB.
DR GO; GO:0090326; P:positive regulation of locomotion involved in locomotory behavior; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR InterPro; IPR007258; Vps52.
DR PANTHER; PTHR14190; PTHR14190; 1.
DR Pfam; PF04129; Vps52; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Golgi apparatus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..702
FT /note="Vacuolar protein sorting-associated protein 52
FT homolog"
FT /id="PRO_0000421176"
FT COILED 505..535
FT /evidence="ECO:0000255"
FT CONFLICT 342
FT /note="A -> V (in Ref. 1; ADO17797)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 702 AA; 80708 MW; ED057C30BA432C75 CRC64;
MPRTRVNLQK SEANDRSFTI SSLEFCLSQL RKADPNLVKK AIASGDGLTE SKNDVSTRLS
EAHRYSVQQC LDNSEQLAQL HNQLVHCDNV FERLQATLYS FQDNLGSIGQ DMKNLQLQSH
HIHQELENRQ KVRVELSQFV DDIAVSQTMM KTINDTDAND RGFLEALHEL HHKITLILQR
GNGDAVAVND TMPILEGLKL KAVVKVREWL LQKMFQFRKP LSNYQVFQHQ LLKCRFFYEF
LLHHDLISAK ELQDEYIDTI SKMFFTYFKA YATRLFKLAM KDVATKEDAL GSIDFAKPAG
LGAIFSSKQH VVRNKATVFS IGQRHQILSD DFLGALIVPH AATQNHQSYQ FEALFRSIQL
AFVDHYSHEY LFITDFFLVS NDEAIELHNK AMARAMSVVL KSCEEQIALS WDAISLHLCI
CLCDKFTEVL AEREVPEVSD YWNTVTSFLW TRLNLVMSQH YESVKSVDLK KLMHSGSLDA
RPHFIVRRYA ELTSAHLMIA KASGKEMGAK MEAVLENSED SIEQLLTRMS AMQQTQKNKH
VFLINNYDLI LSIIDNEESK HTKIYAIVHE LEQKSIDDFV EEMLEPHIGY MIKFVNECES
LIVQGHTQLL VRYNDKVGTV VANFNAKWRP AVDSINSECI QLFTNFSLGT TILQTIFTKY
VQYINRFTKI LSHDVFAKNP VCSQLVNVHQ VMLEIKRFKP AY
