VPS53_CAEEL
ID VPS53_CAEEL Reviewed; 798 AA.
AC P34561; E5QCF9;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 4.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Vacuolar protein sorting-associated protein 53 homolog;
GN Name=vps-53 {ECO:0000312|WormBase:T05G5.8a};
GN ORFNames=T05G5.8 {ECO:0000312|WormBase:T05G5.8a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, IDENTIFICATION IN THE GARP COMPLEX, INTERACTION WITH VPS-51;
RP VPS-52 AND VPS-54, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=21613545; DOI=10.1091/mbc.e10-06-0493;
RA Luo L., Hannemann M., Koenig S., Hegermann J., Ailion M., Cho M.K.,
RA Sasidharan N., Zweckstetter M., Rensing S.A., Eimer S.;
RT "The Caenorhabditis elegans GARP complex contains the conserved Vps51
RT subunit and is required to maintain lysosomal morphology.";
RL Mol. Biol. Cell 22:2564-2578(2011).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=27191843; DOI=10.1371/journal.pgen.1006074;
RA Topalidou I., Cattin-Ortola J., Pappas A.L., Cooper K., Merrihew G.E.,
RA MacCoss M.J., Ailion M.;
RT "The EARP complex and its interactor eipr-1 are required for cargo sorting
RT to dense-core vesicles.";
RL PLoS Genet. 12:E1006074-E1006074(2016).
CC -!- FUNCTION: Acts as component of the GARP complex that is involved in
CC retrograde transport from early and late endosomes to the trans-Golgi
CC network (TGN) (PubMed:21613545). The GARP complex facilitates tethering
CC as well as SNARE complex assembly at the Golgi (PubMed:21613545). Plays
CC a role in the trafficking of cargo to dense-core vesicles, probably
CC through association with the EARP-interacting protein eipr-1
CC (PubMed:27191843). Important for neuronal function (PubMed:27191843).
CC {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:27191843}.
CC -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC complex, also called VFT (VPS fifty-three) complex, composed of vps-51,
CC vps-52, vps-53 and vps-54. Within the complex interacts with vps-51,
CC vps-52 and vps-54 (PubMed:21613545). {ECO:0000269|PubMed:21613545}.
CC -!- INTERACTION:
CC P34561; O01839: vps-51; NbExp=2; IntAct=EBI-6394890, EBI-313277;
CC P34561; G5EFV8: vps-52; NbExp=3; IntAct=EBI-6394890, EBI-318981;
CC P34561; Q22639: vps-54; NbExp=5; IntAct=EBI-6394890, EBI-6395200;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Endosome
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Perikaryon {ECO:0000269|PubMed:27191843}. Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:27191843}. Note=Co-localizes with rab-2 to
CC perinuclear puncta in the perikaryon. {ECO:0000269|PubMed:27191843}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:T05G5.8a};
CC IsoId=P34561-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:T05G5.8b};
CC IsoId=P34561-2; Sequence=VSP_043942;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed, with particularly strong
CC expression in neuronal cells (PubMed:21613545). Specifically expressed
CC in head and tail neurons and in the pharynx and ventral cord motor
CC neurons (PubMed:27191843). {ECO:0000269|PubMed:21613545,
CC ECO:0000269|PubMed:27191843}.
CC -!- DISRUPTION PHENOTYPE: Mutants are viable but display reduced brood size
CC and enlarged lysosomes (PubMed:21613545). Egg-laying defect, slow, but
CC coordinated locomotion, and reduced levels of unprocessed and processed
CC cargo in the motor neuron axon of the dorsal nerve cord
CC (PubMed:27191843). {ECO:0000269|PubMed:21613545,
CC ECO:0000269|PubMed:27191843}.
CC -!- SIMILARITY: Belongs to the VPS53 family. {ECO:0000305}.
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DR EMBL; BX284603; CAA81595.3; -; Genomic_DNA.
DR EMBL; BX284603; CBY25199.2; -; Genomic_DNA.
DR PIR; D88564; D88564.
DR PIR; S41008; S41008.
DR RefSeq; NP_001255027.2; NM_001268098.2. [P34561-1]
DR RefSeq; NP_001255028.2; NM_001268099.2. [P34561-2]
DR AlphaFoldDB; P34561; -.
DR SMR; P34561; -.
DR BioGRID; 41572; 4.
DR ComplexPortal; CPX-365; GARP complex.
DR IntAct; P34561; 7.
DR STRING; 6239.T05G5.8a.2; -.
DR EPD; P34561; -.
DR PaxDb; P34561; -.
DR PeptideAtlas; P34561; -.
DR PRIDE; P34561; -.
DR EnsemblMetazoa; T05G5.8a.1; T05G5.8a.1; WBGene00011502. [P34561-1]
DR EnsemblMetazoa; T05G5.8b.1; T05G5.8b.1; WBGene00011502. [P34561-2]
DR GeneID; 176377; -.
DR KEGG; cel:CELE_T05G5.8; -.
DR UCSC; T05G5.8.2; c. elegans. [P34561-1]
DR CTD; 176377; -.
DR WormBase; T05G5.8a; CE47826; WBGene00011502; vps-53. [P34561-1]
DR WormBase; T05G5.8b; CE47898; WBGene00011502; vps-53. [P34561-2]
DR eggNOG; KOG2180; Eukaryota.
DR GeneTree; ENSGT00390000015165; -.
DR HOGENOM; CLU_007339_1_0_1; -.
DR InParanoid; P34561; -.
DR OMA; YKFAEAK; -.
DR OrthoDB; 1379600at2759; -.
DR PhylomeDB; P34561; -.
DR PRO; PR:P34561; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00011502; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000938; C:GARP complex; IDA:UniProtKB.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:1904810; P:negative regulation of dense core granule transport; IMP:UniProtKB.
DR GO; GO:1904811; P:positive regulation of dense core granule transport; IMP:UniProtKB.
DR GO; GO:0090326; P:positive regulation of locomotion involved in locomotory behavior; IMP:UniProtKB.
DR GO; GO:0060378; P:regulation of brood size; IMP:UniProtKB.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR Gene3D; 1.10.357.110; -; 1.
DR InterPro; IPR039766; Vps53.
DR InterPro; IPR038260; Vps53_C_sf.
DR InterPro; IPR007234; Vps53_N.
DR PANTHER; PTHR12820; PTHR12820; 1.
DR Pfam; PF04100; Vps53_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Endosome; Golgi apparatus; Membrane;
KW Reference proteome.
FT CHAIN 1..798
FT /note="Vacuolar protein sorting-associated protein 53
FT homolog"
FT /id="PRO_0000065447"
FT VAR_SEQ 1..224
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_043942"
SQ SEQUENCE 798 AA; 89229 MW; 80076BB570140CA7 CRC64;
MEEPTTSELK LSDNVMNEIS DMCITEYCKP NMSLMAQINE LFPTEQSLTQ LDSIIASVEG
EIGELDNELA YLVETNANVS ERGEEALKHA QDAMIELEKS IGSIRERTKS SDEIVREMTR
DIKQLDIAKR NLTASITTLH HLHILLTGVE SLGAWVDKKD YSSIARQLPA ILNVLQLFDA
YKESDQIANL SGQLDKLKAS LTIQLAKDLK NAFQTGQLSD RITDMCRVAA ALEGNVKENF
VKWFIEQQLS EYVIIYADNE EGAWLDKVDD RYKWFVRKLT DFERAGLSNI FPADWHMGRR
LTSEFCTVTR DILYRIMTRR RQDLDWKLLG HAIQHTKMFE ALLTKRFPEK DGISFEKAIW
SVFDTFLDVF INAQEKTLNE FLDTCASKIR SGEEKPSRES STHAVPFPSS ADMFLLLKKV
ITESSKLSSE PDALIRDVIG VVRVCLRGYA TSCLVAFLPS LGSQQSGAAN LFSLIREEIA
YPRLTPDQQF LVCCILATAD WCAETSIQLQ EKLSQRIPGV DISQETEAFY SITNQSLQVL
VQDVESTCDA ALQSISKVNW TAVDCVGDES PFIGSMRAHL RQAVPLIRDM LSDRRKYFAH
FCLKLATQLA HKFVGSLFRC RTISTHGAEQ LLLDTHSLKT FLLSVPSIDS IINSKPPTAY
VTSVNAALTK AEMILKVVMC SLETVDEFVE QYIKLLPASD AAEMQKVLEM KGVKRQEHSA
VLNAYRLKIG ASGSDPIQQS NSLTSRIGGA LPTVGSAASV SEAFNAVVSM AADGLSDQAV
TSSIDKLKRF ERLVKRQL
