VPS54_CAEEL
ID VPS54_CAEEL Reviewed; 1058 AA.
AC Q22639;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Vacuolar protein sorting-associated protein 54;
GN Name=vps-54 {ECO:0000312|WormBase:T21C9.2a};
GN ORFNames=T21C9.2 {ECO:0000312|WormBase:T21C9.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, IDENTIFICATION IN THE GARP COMPLEX, INTERACTION WITH VPS-52 AND
RP VPS-53, AND DISRUPTION PHENOTYPE.
RX PubMed=21613545; DOI=10.1091/mbc.e10-06-0493;
RA Luo L., Hannemann M., Koenig S., Hegermann J., Ailion M., Cho M.K.,
RA Sasidharan N., Zweckstetter M., Rensing S.A., Eimer S.;
RT "The Caenorhabditis elegans GARP complex contains the conserved Vps51
RT subunit and is required to maintain lysosomal morphology.";
RL Mol. Biol. Cell 22:2564-2578(2011).
CC -!- FUNCTION: Acts as component of the GARP complex that is involved in
CC retrograde transport from early and late endosomes to the trans-Golgi
CC network (TGN). The GARP complex facilitates tethering as well as SNARE
CC complex assembly at the Golgi. {ECO:0000269|PubMed:21613545}.
CC -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC complex, also called VFT (VPS fifty-three) complex, composed of vps-51,
CC vps-52, vps-53 and vps-54. Within the complex interacts with vps-52 and
CC vps-53 (PubMed:21613545). {ECO:0000269|PubMed:21613545}.
CC -!- INTERACTION:
CC Q22639; G5EFV8: vps-52; NbExp=5; IntAct=EBI-6395200, EBI-318981;
CC Q22639; P34561: vps-53; NbExp=5; IntAct=EBI-6395200, EBI-6394890;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Mutants are viable but display reduced brood size
CC and enlarged lysosomes. {ECO:0000269|PubMed:21613545}.
CC -!- SIMILARITY: Belongs to the VPS54 family. {ECO:0000305}.
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DR EMBL; BX284605; CAA97331.2; -; Genomic_DNA.
DR PIR; T25068; T25068.
DR RefSeq; NP_001256226.1; NM_001269297.1.
DR AlphaFoldDB; Q22639; -.
DR SMR; Q22639; -.
DR BioGRID; 44502; 5.
DR ComplexPortal; CPX-365; GARP complex.
DR IntAct; Q22639; 5.
DR STRING; 6239.T21C9.2a; -.
DR iPTMnet; Q22639; -.
DR EPD; Q22639; -.
DR PaxDb; Q22639; -.
DR PRIDE; Q22639; -.
DR EnsemblMetazoa; T21C9.2a.1; T21C9.2a.1; WBGene00006934.
DR GeneID; 179473; -.
DR KEGG; cel:CELE_T21C9.2; -.
DR UCSC; T21C9.2; c. elegans.
DR CTD; 179473; -.
DR WormBase; T21C9.2a; CE31365; WBGene00006934; vps-54.
DR eggNOG; KOG2115; Eukaryota.
DR GeneTree; ENSGT00390000000583; -.
DR HOGENOM; CLU_005185_1_0_1; -.
DR InParanoid; Q22639; -.
DR OMA; FSFVQSY; -.
DR OrthoDB; 449680at2759; -.
DR PhylomeDB; Q22639; -.
DR PRO; PR:Q22639; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00006934; Expressed in germ line (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q22639; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0000938; C:GARP complex; IPI:WormBase.
DR GO; GO:0019905; F:syntaxin binding; IPI:WormBase.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR InterPro; IPR039745; Vps54.
DR InterPro; IPR012501; Vps54_C.
DR InterPro; IPR019515; VPS54_N.
DR PANTHER; PTHR12965; PTHR12965; 1.
DR Pfam; PF07928; Vps54; 1.
DR Pfam; PF10475; Vps54_N; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Golgi apparatus; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..1058
FT /note="Vacuolar protein sorting-associated protein 54"
FT /id="PRO_0000148735"
FT COILED 369..389
FT /evidence="ECO:0000255"
SQ SEQUENCE 1058 AA; 119182 MW; C65EF22D698C8702 CRC64;
MKSMDDRTVG LISASSSRAS LKIQPEIGYP RICDYCQPII ELMTASEFAR HIRQDHTTKE
GGSFLCRYGE HGVCQKLPLE GVCDLDFEAH IRRCHTSSQP AGYSSPTSSL VASYTEDSEE
TASLRSIRLT SDRDTPTIEK KKFTLHSFTQ NLSAVLADPS RSRSDLTTFF TRHWGDSFVP
TQPVPQSKRL ARLADSAFDS YCQSAGESYR RYQAIKRALR LSHADGGVGI GDERQDAEDL
PTIFIDPRFT LGDSSTFSDV FTVPANDDLD ALKQTLSGRN VIPATPLEIA TNRKPGEFRD
YEALQNRLEM MHDVVDGRLA GKLVAKTDDF WQVVRSYSGL QEQLANALQC VMVVRKNLKR
VDELVCDQSK KIVEVHERYE QKKKLLAKLH DISCLREAQS TVQMMLSQGD YPKAIECIET
SLDVLSKELN GVTCFRHLAS QLRELYTVIG RMMNEDFASL IQKELGVKPE AGTMIQAEGE
LSAVLLGLMR MRKYSFIAVL REEIIEGVKS VMRQVIKNQI LNNGVDLNDF DPSLTQLGEP
VRRMKHADFL KTVKAVMDEE FYFCKRLEAL QDILLETVER ANPLHRHGSE DIIVERIEEA
KDIHESESDD EVGTTFSKSA SSGGFSVGGS ALSSNASATT LLSIEVRSEA FLRHVLPLIA
EFGHQCAQQR ISRLLIARAK NASVTEATTP TQLSECIKLV KEFQSKCDKE GWYSTQNQKV
GGLGRSVNKL SMDYIEKFHA ARKIRIGNML DTELWKATDV SIVDQNIVDM AMETGQLRNT
KRIDDGPIKK SFKRTESAVT IDSTTSTSNQ IQGVIVDEEN YVVVGSSITM IQLLSDYCEA
ISEMPSFSQD WNSRVVELLK TFNSRCCQLI LGAGALQLVG LKTISVRNLA LAGRSLELVC
RFIPMVHDEM DRVLPENRKS LLRYFKQVES EYRDHVNEIA AKLISVIAHY TTNCLGMWDV
KGVIPSPEFQ QICRHMLKFH NGLVGIMPRD QIEALFRQVH ENFKANLREH VTGMGITPHD
PLKYGYVTKD YMFYQQNVKN MESCRNLELE SLNDIMFE
