VPS54_DROME
ID VPS54_DROME Reviewed; 940 AA.
AC Q9VLC0;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Vacuolar protein sorting-associated protein 54;
DE AltName: Full=Protein scattered;
GN Name=scat; ORFNames=CG3766;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=9550716; DOI=10.1242/dev.125.10.1833;
RA Fabrizio J.J., Hime G., Lemmon S.K., Bazinet C.;
RT "Genetic dissection of sperm individualization in Drosophila
RT melanogaster.";
RL Development 125:1833-1843(1998).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-30, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May be involved in retrograde transport from early and late
CC endosomes to late Golgi (By similarity). Required during
CC spermatogenesis for sperm individualization. {ECO:0000250,
CC ECO:0000269|PubMed:9550716}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VPS54 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52774.1; -; Genomic_DNA.
DR EMBL; AY061309; AAL28857.1; -; mRNA.
DR RefSeq; NP_524905.1; NM_080166.4.
DR AlphaFoldDB; Q9VLC0; -.
DR SMR; Q9VLC0; -.
DR BioGRID; 71064; 2.
DR IntAct; Q9VLC0; 2.
DR STRING; 7227.FBpp0079409; -.
DR iPTMnet; Q9VLC0; -.
DR PaxDb; Q9VLC0; -.
DR PRIDE; Q9VLC0; -.
DR EnsemblMetazoa; FBtr0079809; FBpp0079409; FBgn0011232.
DR GeneID; 47942; -.
DR KEGG; dme:Dmel_CG3766; -.
DR UCSC; CG3766-RA; d. melanogaster.
DR CTD; 47942; -.
DR FlyBase; FBgn0011232; scat.
DR VEuPathDB; VectorBase:FBgn0011232; -.
DR eggNOG; KOG2115; Eukaryota.
DR GeneTree; ENSGT00390000000583; -.
DR HOGENOM; CLU_005185_1_0_1; -.
DR InParanoid; Q9VLC0; -.
DR OMA; FSFVQSY; -.
DR OrthoDB; 449680at2759; -.
DR PhylomeDB; Q9VLC0; -.
DR SignaLink; Q9VLC0; -.
DR BioGRID-ORCS; 47942; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 47942; -.
DR PRO; PR:Q9VLC0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011232; Expressed in spermathecum and 45 other tissues.
DR Genevisible; Q9VLC0; DM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0000938; C:GARP complex; IDA:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000138; C:Golgi trans cisterna; IDA:FlyBase.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0001675; P:acrosome assembly; IMP:FlyBase.
DR GO; GO:0032456; P:endocytic recycling; IDA:FlyBase.
DR GO; GO:0006896; P:Golgi to vacuole transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR GO; GO:0007290; P:spermatid nucleus elongation; IMP:FlyBase.
DR InterPro; IPR039745; Vps54.
DR InterPro; IPR012501; Vps54_C.
DR InterPro; IPR019515; VPS54_N.
DR PANTHER; PTHR12965; PTHR12965; 1.
DR Pfam; PF07928; Vps54; 1.
DR Pfam; PF10475; Vps54_N; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; Differentiation; Golgi apparatus;
KW Phosphoprotein; Protein transport; Reference proteome; Spermatogenesis;
KW Transport.
FT CHAIN 1..940
FT /note="Vacuolar protein sorting-associated protein 54"
FT /id="PRO_0000148736"
FT REGION 192..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 299..325
FT /evidence="ECO:0000255"
FT MOD_RES 30
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 940 AA; 105241 MW; 295D78EFE213AD62 CRC64;
MATTRSAGGA ATSVDTGPAA GNSGIRKLST ASVGGIAGGV APSWQSCYYC TREHFKSISD
FVNHLRNRHC TREGGSFVCR YGFNGVCASL PLDGVSDRDY DAHVAKYHVN QHTREMPPEW
GVYSAAQNLP AVLNDPSRGK QSNLFTKKWG EHFVERSHVP PSPRLPDITH ADFTVYLGSI
GKRYRWHERR QQQLERDKPL ENGAQGAPGP GTGGQTPTHL SSVPEIFLKS QLQLHHPATF
KQVFPNYMQT SASSPESHQQ TGRQLQEQLS HYLDMVEVKI AQQVSQKSAA FFHAMTTQHA
ILAEMEQAAD QVRQLRAALA ELHSHSVVDS FKVLRFAQRR QHYNLTLDKL RLMATVHKTQ
PMLQLLLGTQ DYVAALDLIG TTQEILSAEL LGIHCFKHLP MQLSEMEKLI DKMLTTEFER
YAAADLNRPL TDALRETDSV CAEEDKLVAI VMGLLRKQNF SFVQAYQQEA IATIRAIIKQ
LLIEVLARSD SDQEISLTGH GEQALELTLP EWIALLQRSS QALVSILERI KTVVGIMQQT
ADAAVGAQDA VNLIDSEAFL SPGHHEQLKN QLQQLLQAVC HYCHERCANI VSPQSLERSS
ASEQELFQLS EIVDHFGETT RSICGVASVP LQLALKVQAS RYAQRFHSER KQKLSLLLDQ
ERWRQVDIPH EFQRIIERMA AGDYAKPEMG NLISNGAGNP VLLVEGKQPY TLVSASLMLI
RMLYEYGCSA HRLPLLASYH ARNVVDLLRC FNSRSCQLII GAGAMRVAGL KTITSTNLAL
VSRALQLVLW LLPKLKEHFQ AMSGYETIER DYQGHIKEIE NKIHGIVSER LAAQLDAWEA
RPPIPSQTFR HISRHLVKLH EAIAGVLPEA QIHEIYGVVH RNFKDKLREQ LLKLNVNNNG
GPQHGVVTSE LTFYMETLRT LKALPAEQLD NGILEEIWLY
