VPS54_RAT
ID VPS54_RAT Reviewed; 965 AA.
AC Q9JMK8;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Vacuolar protein sorting-associated protein 54;
DE AltName: Full=Vps54-like protein;
GN Name=Vps54; Synonyms=Vps54l;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Louvain;
RX PubMed=12039048; DOI=10.1016/s0378-1119(02)00405-5;
RA Walter L., Stark S., Helou K., Fluegge P., Levan G., Guenther E.;
RT "Identification, characterization and cytogenetic mapping of a yeast Vps54
RT homolog in rat and mouse.";
RL Gene 285:213-220(2002).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=31721635; DOI=10.1091/mbc.e18-07-0469;
RA Topalidou I., Cattin-Ortola J., Hummer B., Asensio C.S., Ailion M.;
RT "EIPR1 controls dense-core vesicle cargo retention and EARP complex
RT localization in insulin-secreting cells.";
RL Mol. Biol. Cell 31:59-79(2020).
CC -!- FUNCTION: Acts as component of the GARP complex that is involved in
CC retrograde transport from early and late endosomes to the trans-Golgi
CC network (TGN). The GARP complex is required for the maintenance of the
CC cycling of mannose 6-phosphate receptors between the TGN and endosomes,
CC this cycling is necessary for proper lysosomal sorting of acid
CC hydrolases such as CTSD. Within the GARP complex, required to tether
CC the complex to the TGN. Not involved in endocytic recycling.
CC {ECO:0000250|UniProtKB:Q9P1Q0}.
CC -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC complex, also called VFT (VPS fifty-three) complex, composed of VPS51,
CC VPS52, VPS53 and VPS54 (By similarity). EIPR1 interacts with GARP
CC complex and mediates its recruitment to the trans-Golgi network (By
CC similarity). Interacts with VPS51 in an EIPR1-independent manner (By
CC similarity). {ECO:0000250|UniProtKB:Q9P1Q0}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250|UniProtKB:Q9P1Q0}. Membrane {ECO:0000269|PubMed:31721635}.
CC Note=Associates with membranes in an EIPR1-independent manner.
CC {ECO:0000269|PubMed:31721635}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed at low level.
CC {ECO:0000269|PubMed:12039048}.
CC -!- SIMILARITY: Belongs to the VPS54 family. {ECO:0000305}.
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DR EMBL; AJ010392; CAB96885.1; -; mRNA.
DR RefSeq; NP_775170.1; NM_173147.1.
DR AlphaFoldDB; Q9JMK8; -.
DR SMR; Q9JMK8; -.
DR STRING; 10116.ENSRNOP00000010264; -.
DR PhosphoSitePlus; Q9JMK8; -.
DR PaxDb; Q9JMK8; -.
DR PeptideAtlas; Q9JMK8; -.
DR PRIDE; Q9JMK8; -.
DR GeneID; 286932; -.
DR KEGG; rno:286932; -.
DR UCSC; RGD:628718; rat.
DR CTD; 51542; -.
DR RGD; 628718; Vps54.
DR eggNOG; KOG2115; Eukaryota.
DR InParanoid; Q9JMK8; -.
DR OrthoDB; 449680at2759; -.
DR PhylomeDB; Q9JMK8; -.
DR Reactome; R-RNO-6811440; Retrograde transport at the Trans-Golgi-Network.
DR PRO; PR:Q9JMK8; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0000938; C:GARP complex; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:RGD.
DR GO; GO:0005802; C:trans-Golgi network; IDA:MGI.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0006896; P:Golgi to vacuole transport; IBA:GO_Central.
DR GO; GO:0048873; P:homeostasis of number of cells within a tissue; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0007041; P:lysosomal transport; ISO:RGD.
DR GO; GO:0050881; P:musculoskeletal movement; ISO:RGD.
DR GO; GO:2000672; P:negative regulation of motor neuron apoptotic process; ISO:RGD.
DR GO; GO:0060052; P:neurofilament cytoskeleton organization; ISO:RGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; ISO:RGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISO:RGD.
DR GO; GO:0048515; P:spermatid differentiation; ISO:RGD.
DR InterPro; IPR039745; Vps54.
DR InterPro; IPR012501; Vps54_C.
DR InterPro; IPR019515; VPS54_N.
DR PANTHER; PTHR12965; PTHR12965; 1.
DR Pfam; PF07928; Vps54; 1.
DR Pfam; PF10475; Vps54_N; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..965
FT /note="Vacuolar protein sorting-associated protein 54"
FT /id="PRO_0000148733"
FT REGION 516..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 228..249
FT /evidence="ECO:0000255"
FT COMPBIAS 517..558
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 8
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P1Q0"
SQ SEQUENCE 965 AA; 108842 MW; F9DAEFA5A9C8203E CRC64;
MASSHSSSPV PQGSSSDVFF KKEVDPTKHV RPVQSLPDVC PKEPTVTDQH RWTVYHSKVN
LPAALNDPTL AKRESDFFTK TWGLGFVDTE VIPSLYLPQI SKEHFIAYQQ EISQREKIHE
RCKNICPPKD TFDRTLLHIH DKSRTDLEQV PKIFMKPDFA LDDSLTFNSV LPWSHFNTAG
GKGNRDAASS KLLQEKLSHY LDIVEVNIAH QISLRSEAFF HAMTSQHELQ DYLKKTSQAV
KMLRDKIAQI DKVMCEGSLQ ILRLALTRNN CVKVSNKLKL MATVHQTQPT VQVLLSTSEF
VGALDLIATT QEVLQQELQG VHSFRHLGSQ LCELEKLIDK MMIAEFSTYS HSDLNRPLEG
ECQVLEEERL VSLVFGLLEQ RKLNFLEIYG EETIITAKNI IKECVINKVA QVEEIDTDAV
VKLADQMRML NFPQWIDLLK DIFSKFTIFL QRVKATLNVI HSVVLSVLDK NQRTRELEEV
SQQRSAGKDN SLDTEVAYLT HEGLFISDAF SEAEPASAAV DTTSQRNTSP HSEPCSSDSV
SEPECTTDSS SSKEQTPASA TLGGVDIIVS EDMRLTDLEL GKLASNIQEL LCNASDVCHD
RAVKFLMSRA KDGFLEKLNS TEFIALSRLM ETYIVDTEQI CGRKSTSLLG ALQSQANKFV
NRFHEERRTK LSLLLDNERW KQADVPAEFQ DLVDSIADGK IALPDKKPAA TEDRKPADVL
VVEGHQYAVV GTVLLLIRII LEYCQCVDNI PSVTTDMLTR LTDLLKYFNS RSCQLVLGAG
ALQVVGLKTI TTKNLALSSR CLQLIVHYIP VIRAHFEARL PRKQWSLLRH FDHITKDYHD
HIGEISSKLI AIMDSLFDKL LSRCEVEAPA PSPCFRNICK QMTKMHEAIF DLLPKEQTQM
LILRINASYK FHLKKQLSHL NVINDGGPQS GFVTADVAFY TGNLQALKGL KDLDLNMAEI
WEQKR
