ID   VPS65_YEAST             Reviewed;         104 AA.
AC   O13554;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Putative uncharacterized protein VPS65;
GN   Name=VPS65; OrderedLocusNames=YLR322W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=12134085; DOI=10.1091/mbc.02-01-0005;
RA   Bonangelino C.J., Chavez E.M., Bonifacino J.S.;
RT   "Genomic screen for vacuolar protein sorting genes in Saccharomyces
RT   cerevisiae.";
RL   Mol. Biol. Cell 13:2486-2501(2002).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Causes a vacuolar protein sorting defect.
CC       {ECO:0000269|PubMed:12134085}.
CC   -!- MISCELLANEOUS: Partially overlaps SFH1. Disruption phenotypes caused by
CC       deletion of this gene may also be a result of a defect in its
CC       overlapping gene. {ECO:0000305}.
CC   -!- CAUTION: Product of a dubious gene prediction unlikely to encode a
CC       functional protein. Because of that it is not part of the S.cerevisiae
CC       S288c complete/reference proteome set. {ECO:0000305|PubMed:24374639}.
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DR   EMBL; U20618; AAB64531.1; -; Genomic_DNA.
DR   PIR; S69310; S69310.
DR   AlphaFoldDB; O13554; -.
DR   DIP; DIP-1902N; -.
DR   IntAct; O13554; 1.
DR   MINT; O13554; -.
DR   STRING; 4932.YLR322W; -.
DR   PaxDb; O13554; -.
DR   PRIDE; O13554; -.
DR   EnsemblFungi; YLR322W_mRNA; YLR322W; YLR322W.
DR   SGD; S000004314; VPS65.
DR   HOGENOM; CLU_2252172_0_0_1; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006623; P:protein targeting to vacuole; HMP:SGD.
PE   5: Uncertain;
KW   Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..104
FT                   /note="Putative uncharacterized protein VPS65"
FT                   /id="PRO_0000299781"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   104 AA;  12162 MW;  A68FCABB5AB6F807 CRC64;
     MRHCIIFIVC ISIVEIRTVH IEFIKEIVVI FRIVDHFSPF MLPCLLSHCK DGDTIIFVCQ
     SVMKVRNISL WNKLVLVRHC VLLCAFLLSF FNVLHSIISI CRIF