VPS8_DROME
ID VPS8_DROME Reviewed; 1229 AA.
AC Q9VRX2; B7FNP6; C5WLL9; D0IQG0;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Vacuolar protein sorting-associated protein 8 homolog {ECO:0000250|UniProtKB:Q8N3P4};
DE AltName: Full=Vacuolar protein sorting 8 {ECO:0000312|FlyBase:FBgn0035704};
GN Name=Vps8 {ECO:0000312|FlyBase:FBgn0035704};
GN ORFNames=CG10144 {ECO:0000312|FlyBase:FBgn0035704};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ACS54286.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ACS54286.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ACK77654.1}, and
RC Testis {ECO:0000312|EMBL:ACS54286.1, ECO:0000312|EMBL:ACY40032.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE CORVET COMPLEX, DISRUPTION PHENOTYPE, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=27253064; DOI=10.7554/elife.14226;
RA Lorincz P., Lakatos Z., Varga A., Maruzs T., Simon-Vecsei Z., Darula Z.,
RA Benko P., Csordas G., Lippai M., Ando I., Hegedus K., Medzihradszky K.F.,
RA Takats S., Juhasz G.;
RT "MiniCORVET is a Vps8-containing early endosomal tether in Drosophila.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Plays a role in vesicle-mediated protein trafficking to
CC lysosomal compartments and in membrane docking/fusion reactions of late
CC endosomes/lysosomes probably as part of the class C core
CC vacuole/endosome tethering (CORVET) complex. Specifically required for
CC endocytic trafficking in a subset of cells, such as hemocytes and
CC nephrocytes, which are highly active in endocytosis.
CC {ECO:0000269|PubMed:27253064}.
CC -!- SUBUNIT: Component of the class C core vacuole/endosome tethering
CC (CORVET) complex composed of at least dor/Vps18, Vps16A, Vps8 and
CC car/Vps33A; unlike in other species, Vps11 is not part of the
CC Drosophila complex. {ECO:0000269|PubMed:27253064}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000269|PubMed:27253064}.
CC Note=Localization to the early endosome is dependent on Rab5 and class
CC C core vacuole/endosome tethering (CORVET) complex subunits such as
CC dor/Vps18, car/Vps33A and Vps16A. {ECO:0000269|PubMed:27253064}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the larva, in the pupal wings and the
CC adult fly (at protein level). High levels of expression are found in
CC larval hemocytes and garland nephrocytes (at protein level).
CC {ECO:0000269|PubMed:27253064}.
CC -!- DISRUPTION PHENOTYPE: Semilethal: animals can complete metamorphosis,
CC but most of them die as pharate adults unable to emerge from the pupal
CC case; surviving flies are weak, fail to unfold their wings properly and
CC die within 24 hr. The eye color is similar to wild type flies. In
CC larvae, results in the development of hemocyte-derived melanotic tumors
CC in body cavities, accumulation of crystal cells and lamellocytes and
CC disorganization of the sessile hemocyte compartment which might be
CC connected to the abnormal increase of circulating hemocytes. In garland
CC cells, uptake from the hemolymph, endosome formation and endo-lysosomal
CC trafficking are defective and result in enlarged cells; late endosomes
CC and endolysosomes are fragmented and erroneously distributed in the
CC cytoplasm instead of being found in a layer directly beneath peripheral
CC early endosomes. In hemocytes, acidification of endocytic vacuoles
CC containing bacteria is defective. In wing imaginal disks, there are no
CC endosome defects. {ECO:0000269|PubMed:27253064}.
CC -!- SIMILARITY: Belongs to the VPS8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACK77654.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ACY40032.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing at the N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF50660.1; -; Genomic_DNA.
DR EMBL; BT053736; ACK77654.1; ALT_FRAME; mRNA.
DR EMBL; BT088834; ACS54286.1; -; mRNA.
DR EMBL; BT100198; ACY40032.1; ALT_SEQ; mRNA.
DR RefSeq; NP_648048.1; NM_139791.3.
DR AlphaFoldDB; Q9VRX2; -.
DR IntAct; Q9VRX2; 1.
DR STRING; 7227.FBpp0076695; -.
DR PaxDb; Q9VRX2; -.
DR EnsemblMetazoa; FBtr0076986; FBpp0076695; FBgn0035704.
DR GeneID; 38735; -.
DR KEGG; dme:Dmel_CG10144; -.
DR UCSC; CG10144-RA; d. melanogaster.
DR CTD; 23355; -.
DR FlyBase; FBgn0035704; Vps8.
DR VEuPathDB; VectorBase:FBgn0035704; -.
DR eggNOG; KOG2079; Eukaryota.
DR GeneTree; ENSGT00390000010672; -.
DR HOGENOM; CLU_000917_1_2_1; -.
DR InParanoid; Q9VRX2; -.
DR OMA; WAHQDKH; -.
DR OrthoDB; 22166at2759; -.
DR PhylomeDB; Q9VRX2; -.
DR BioGRID-ORCS; 38735; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38735; -.
DR PRO; PR:Q9VRX2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035704; Expressed in embryonic/larval hemocyte (Drosophila) and 25 other tissues.
DR Genevisible; Q9VRX2; DM.
DR GO; GO:0033263; C:CORVET complex; IDA:UniProtKB.
DR GO; GO:0005769; C:early endosome; IDA:UniProtKB.
DR GO; GO:0030897; C:HOPS complex; IBA:GO_Central.
DR GO; GO:0005770; C:late endosome; IBA:GO_Central.
DR GO; GO:0051020; F:GTPase binding; ISS:FlyBase.
DR GO; GO:0008270; F:zinc ion binding; ISM:FlyBase.
DR GO; GO:0006897; P:endocytosis; IMP:UniProtKB.
DR GO; GO:0016197; P:endosomal transport; ISS:FlyBase.
DR GO; GO:0034058; P:endosomal vesicle fusion; IDA:UniProtKB.
DR GO; GO:0007032; P:endosome organization; IDA:UniProtKB.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR000547; Clathrin_H-chain/VPS_repeat.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR045111; Vps41/Vps8.
DR InterPro; IPR025941; Vps8_central_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR12616; PTHR12616; 1.
DR Pfam; PF00637; Clathrin; 1.
DR Pfam; PF12816; Vps8; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR PROSITE; PS50236; CHCR; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Endosome; Metal-binding; Protein transport; Reference proteome; Transport;
KW Zinc; Zinc-finger.
FT CHAIN 1..1229
FT /note="Vacuolar protein sorting-associated protein 8
FT homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000444757"
FT REPEAT 901..1063
FT /note="CHCR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01006"
FT ZN_FING 1148..1189
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 67..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 460
FT /note="V -> L (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 554
FT /note="L -> H (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 646
FT /note="D -> E (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 666
FT /note="E -> G (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 857
FT /note="S -> C (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 930
FT /note="L -> V (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
FT CONFLICT 960
FT /note="S -> A (in Ref. 3; ACS54286/ACY40032)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1229 AA; 137922 MW; 256B978BC5B4B71F CRC64;
MSELKAPSLQ SLLESERGST DSLLAESLQL DFEDLDDAEF AIPPTDVLPT LEAVLSEFEA
DSDVASEFGM PVPHATPTPS IGEDSTIRTD GRGGGGSIMR YTLLHGISAQ LSSAAERVNA
GAASSCAVAA FIAIGTSHGH ILNFDVTQTL RWAHQDKHGQ GAVASLAFNA DSTRLLAGFS
RGLVAMLDTH TGDVLRELFD VITPNTGVLH VKWTSRSSLA LCADAGGSVW SLSFTRKLGI
RGCQSRCLFS GARGEVCAVE PLIMDSQGRH ELDQYCIVAL ATLSKYFIVT VRPRLRVIKY
HVLQGPPDCL PLLAWHLVLI QAADTSRSVD PVIVVGRGNQ LFFHQLFVSN GRITLLYLRH
VQLQGSLLSA HWLGPKCVAS LDTAEILHLV DVRSSKELEC MDMANAGLVY GSAQFKGLAT
GGNVSPAFAL AGSNACYNSV VSRGTQLYVL GARSLHIIGV RTWSERISFL VKHHRWQEAC
QLALDGYIAS VDRPRKRAQA KERIIMLFKE YIANSARAPE YCLGAIVNCL ITVGELDLLW
TQLWEKLHNS STELFLQHIS EHIEKETIHS VNPVISQALV DYWLEHSPAK LEQLILKLDW
MCLDLNQVLK AVKKHRLFRA QIYLNTQALN DYTAALTELL PLVTPDETDL GNCLLVYVSS
CLAGREYPSG EIPVELVHQV KHDVLRCLTS QHSKENAGDE LPYPYLRALL KFDTRETLNV
ISLAFQEREF SNELGISHRK RIINLLLEIM SPENATWAEI GCLLNFIAQQ ISMQCLPRDR
QLLERVLSHL AQEEIANESS RQHSERENAW HELLSSNCLA EISSDEEQLR LAEKAKCYCV
VEYLLEKLER YDTILDSYIR NEARHETMFA YMERHVASPK RSIFRQLKRN LRELLTINAK
ETTRLLSLHY PEKINELLDN LRREENLLYL FLKCLNDRKS ELEASQMELL LELYCKMESS
STVEEFLRSN SGYRLENAIA IAESHHLNRS VIYLYEKQES YAKAFELSME LLKSAAGEEA
AKEAQTISAL LARSVETLPA QELERCWFAL LQYILPHQEL QSITKSLLHE ASQHIDLHNL
VQLIMNTHNV STSFGDIKDL LMGMLDSSRH KTEALRASAG ALCQDLHLKF VKRYQHAHRG
LWVTTTKCSM CRQRLYDHSQ VLIFGGCGHG IHEQCMEESE TQFEECPRCF TAIPDQSIGL
PRPNKNLISI SSSLEMGALQ LKAPPRRFI
