VPS8_SCHPO
ID VPS8_SCHPO Reviewed; 1272 AA.
AC O13756;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Vacuolar protein sorting-associated protein 8;
DE AltName: Full=Vacuolar protein-targeting protein 8;
GN Name=vps8; ORFNames=SPAC17A2.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in the retention of proteins to the late-Golgi.
CC Plays an integral role in the complex vacuolar protein sorting process
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000269|PubMed:16823372}.
CC Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the VPS8 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB16561.1; -; Genomic_DNA.
DR PIR; T37807; T37807.
DR RefSeq; NP_594240.1; NM_001019663.2.
DR AlphaFoldDB; O13756; -.
DR SMR; O13756; -.
DR BioGRID; 278612; 20.
DR STRING; 4896.SPAC17A2.06c.1; -.
DR iPTMnet; O13756; -.
DR MaxQB; O13756; -.
DR PaxDb; O13756; -.
DR PRIDE; O13756; -.
DR EnsemblFungi; SPAC17A2.06c.1; SPAC17A2.06c.1:pep; SPAC17A2.06c.
DR GeneID; 2542136; -.
DR KEGG; spo:SPAC17A2.06c; -.
DR PomBase; SPAC17A2.06c; vps8.
DR VEuPathDB; FungiDB:SPAC17A2.06c; -.
DR eggNOG; KOG2079; Eukaryota.
DR HOGENOM; CLU_263077_0_0_1; -.
DR InParanoid; O13756; -.
DR OMA; WAHQDKH; -.
DR PhylomeDB; O13756; -.
DR PRO; PR:O13756; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0033263; C:CORVET complex; ISO:PomBase.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0030897; C:HOPS complex; IBA:GO_Central.
DR GO; GO:0005770; C:late endosome; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; NAS:PomBase.
DR GO; GO:0034058; P:endosomal vesicle fusion; IBA:GO_Central.
DR GO; GO:0045324; P:late endosome to vacuole transport; ISO:PomBase.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045111; Vps41/Vps8.
DR InterPro; IPR025941; Vps8_central_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR12616; PTHR12616; 1.
DR Pfam; PF12816; Vps8; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Golgi apparatus; Protein transport; Reference proteome; Repeat;
KW Transport; WD repeat.
FT CHAIN 1..1272
FT /note="Vacuolar protein sorting-associated protein 8"
FT /id="PRO_0000372426"
FT REPEAT 91..130
FT /note="WD 1"
FT REPEAT 137..176
FT /note="WD 2"
FT REPEAT 183..223
FT /note="WD 3"
SQ SEQUENCE 1272 AA; 146210 MW; 3666AC89545908E7 CRC64;
MQSKYGRSSL KIKNGDDTRT LRALSFSKDS VNSSSFDNEA ASTTLGISTA IHASNRPFRQ
DEYSWNDCWM RWGQLRRVSS QLRELRFTMN DQPATVTSIA YQGTLLVAGT SSGHVLLNDW
RTNDFQILKP GLSSNESVTL PVTSLAISNS KRIVCQGHAG GIIFVWDVSR KPPLQLFTIN
QHSEDSVLTH LVFNGNSDDV LLSTDHLGKI AVHEFYNLVI NKHCTSWNLD MSKSNSLNLD
SIIVDTSSIS FEELHITGVK HFSFVVLQSL QSIQIVTLFP KTTLIYEFKY PAGIVSSACH
SFSSSIVHKN GENTKLHAFF ATAYNNNLRL YSVTFHKGYM SLQLMDRKKF AKAIWKLWWF
PSSSIIIILF DDMELAFVNS MSLDIIGTST ILDKSLLRWD WYSNSLAAIG VSQTVFPFIS
SSLCISPRYM FVVNSETVSV GSFLMVSEQL QMLSERYGFF DSIKFGSYCV SNLSIFPKLL
LEKTSVINLI MALYVKLLKS LARDFPARSP FEATSQKTND YELEVQHLFT WTCELYSENF
NTVISSFIIA NSLGILPEII EQIIPVFTRV GKVYVVLEAL FDLILSQRFT NPSPQLQHHI
LNYLNDCKRL QDMDKLIPCI EYQSLDLDFI TKFSRSKGLF DSLCYVSIYA FNDYSIPLVQ
FLNLLKSDID SPSEETSINV EKGFHFLLYS LTGMKYPLGH SNNINDAYVV IHTLLKVIFS
VVPLPFDVPV DNSVDFPYLR LFLEKHADDF FRCLEVAFDY PYFSLEKVTI AKKLLDTVNR
QWILECISQL YEKKKSVSQS YYIFVSHVTA NYSNQLLFSQ SFYQGILDGL CNIDVPDDKR
EVVEAAIEEL LTVYEHFDIA TTLLSCWDHQ LYQVILHISF KCGRYEDYIE AILALWKQKG
QKHSFVSESS EALISLVFNN LQTINRFPGH RERYVSTLIS HCGDLFAINH VCFVRNSLKY
LLDDFTKVIT KTFSIKSIRL QFLSLIIYEI TYEQLSSWYR ERTILSFLEL VSFDQGDVKM
LELLRLHPKL VRLQGLMEIL NKNDCIESCI FVYRELAEYK LALSHVSKYI EKSMSVFDLE
KSDDMTSRRI ERLCLHLKTV VPLFEQCTKE VSAEDVTNFW LELVFTLLLV YIKLSLKGSI
NSQITFKDFQ QELSLCIENL LDSFLSKTNS YKIALNTVLV RICEEASRNE HSDQYLRKLL
LKLITDLYIN HDITKECFLL WDMKQFYEIR STLLQNASGV LVEDPKLQKN AKGQYTSKLK
VYFSGLIERE DL
