ID   VPSU_BPP4               Reviewed;         190 AA.
AC   P05460; Q96207;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 3.
DT   29-SEP-2021, entry version 82.
DE   RecName: Full=Polarity suppression protein;
DE   AltName: Full=Amber mutation-suppressing protein;
GN   Name=psu;
OS   Enterobacteria phage P4 (Bacteriophage P4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales.
OX   NCBI_TaxID=10680;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3295254; DOI=10.1016/0022-2836(86)90029-x;
RA   Dale E.C., Christie G.E., Calendar R.;
RT   "Organization and expression of the satellite bacteriophage P4 late gene
RT   cluster.";
RL   J. Mol. Biol. 192:793-803(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2183201; DOI=10.1093/nar/18.6.1649;
RA   Halling C., Calendar R., Christie G.E., Dale E.C., Deho G., Finkel S.,
RA   Flensburg J., Ghisotti D., Kahn M.L., Lane K.B., Lin C.-S., Lindqvist B.H.,
RA   Pierson L.S., Six E.W., Sunshine M.G., Ziermann R.;
RT   "DNA sequence of satellite bacteriophage P4.";
RL   Nucleic Acids Res. 18:1649-1649(1990).
RN   [3]
RP   SEQUENCE REVISION TO 169.
RA   Halling C.;
RL   Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61.
RX   PubMed=6095206; DOI=10.1093/nar/12.22.8667;
RA   Lin C.-S.;
RT   "Nucleotide sequence of the essential region of bacteriophage P4.";
RL   Nucleic Acids Res. 12:8667-8684(1984).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
RX   PubMed=2193910; DOI=10.1128/jb.172.7.3541-3548.1990;
RA   Halling C., Sunshine M.G., Lane K.B., Six E.W., Calendar R.;
RT   "A mutation of the transactivation gene of satellite bacteriophage P4 that
RT   suppresses the rpoA109 mutation of Escherichia coli.";
RL   J. Bacteriol. 172:3541-3548(1990).
CC   -!- FUNCTION: Late protein which functions to suppress polarity of amber
CC       mutations in the late genes of the P2 helper phage.
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DR   EMBL; M29650; AAA32432.1; -; Genomic_DNA.
DR   EMBL; X51522; CAA35906.1; -; Genomic_DNA.
DR   EMBL; X02534; CAA26374.1; -; Genomic_DNA.
DR   EMBL; M29479; AAA32435.1; -; Genomic_DNA.
DR   PIR; S06887; PUBPP4.
DR   RefSeq; NP_042044.1; NC_001609.1.
DR   PDB; 3RX6; X-ray; 2.04 A; A=1-190.
DR   PDB; 4DVD; X-ray; 3.00 A; A=1-190.
DR   PDBsum; 3RX6; -.
DR   PDBsum; 4DVD; -.
DR   SMR; P05460; -.
DR   GeneID; 1261094; -.
DR   KEGG; vg:1261094; -.
DR   Proteomes; UP000009093; Genome.
DR   GO; GO:1990216; P:positive regulation by symbiont of host transcription; IDA:CACAO.
DR   InterPro; IPR010006; Phage_P4_Psu.
DR   Pfam; PF07455; Psu; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Late protein; Reference proteome.
FT   CHAIN           1..190
FT                   /note="Polarity suppression protein"
FT                   /id="PRO_0000165227"
FT   CONFLICT        18
FT                   /note="A -> T (in Ref. 4; CAA26374)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        169
FT                   /note="F -> S (in Ref. 1; AAA32432)"
FT                   /evidence="ECO:0000305"
FT   HELIX           5..39
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   STRAND          40..42
FT                   /evidence="ECO:0007829|PDB:4DVD"
FT   HELIX           45..99
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           101..104
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           107..109
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           112..134
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   TURN            144..146
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           147..153
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           159..165
FT                   /evidence="ECO:0007829|PDB:3RX6"
FT   HELIX           171..186
FT                   /evidence="ECO:0007829|PDB:3RX6"
SQ   SEQUENCE   190 AA;  21366 MW;  D09CEAE7135A3C06 CRC64;
     MESTALQQAF DTCQNNKAAW LQRKNELAAA EQEYLRLLSG EGRNVSRLDE LRNIIEVRKW
     QVNQAAGRYI RSHEAVQHIS IRDRLNDFMQ QHGTALAAAL APELMGYSEL TAIARNCAIQ
     RATDALREAL LSWLAKGEKI NYSAQDSDIL TTIGFRPDVA SVDDSREKFT PAQNMIFSRK
     SAQLASRQSV