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VPU_HV1ET
ID   VPU_HV1ET               Reviewed;          86 AA.
AC   Q75007;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 99.
DE   RecName: Full=Protein Vpu {ECO:0000255|HAMAP-Rule:MF_04082};
DE   AltName: Full=U ORF protein {ECO:0000255|HAMAP-Rule:MF_04082};
DE   AltName: Full=Viral protein U {ECO:0000255|HAMAP-Rule:MF_04082};
GN   Name=vpu {ECO:0000255|HAMAP-Rule:MF_04082};
OS   Human immunodeficiency virus type 1 group M subtype C (isolate ETH2220)
OS   (HIV-1).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX   NCBI_TaxID=388796;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C2220;
RX   PubMed=8891112; DOI=10.1089/aid.1996.12.1329;
RA   Salminen M.O., Johansson B., Sonnerborg A., Ayehunie S., Gotte D.,
RA   Leinikki P., Burke D.S., McCutchan F.E.;
RT   "Full-length sequence of an ethiopian human immunodeficiency virus type 1
RT   (HIV-1) isolate of genetic subtype C.";
RL   AIDS Res. Hum. Retroviruses 12:1329-1339(1996).
CC   -!- FUNCTION: Enhances virion budding by targeting host CD4 and
CC       Tetherin/BST2 to proteasome degradation. Degradation of CD4 prevents
CC       any unwanted premature interactions between viral Env and its host
CC       receptor CD4 in the endoplasmic reticulum. Degradation of
CC       antiretroviral protein Tetherin/BST2 is important for virion budding,
CC       as BST2 tethers new viral particles to the host cell membrane.
CC       Mechanistically, Vpu bridges either CD4 or BST2 to BTRC, a substrate
CC       recognition subunit of the Skp1/Cullin/F-box protein E3 ubiquitin
CC       ligase, induces their ubiquitination and subsequent proteasomal
CC       degradation. The alteration of the E3 ligase specificity by Vpu seems
CC       to promote the degradation of host IKBKB, leading to NF-kappa-B down-
CC       regulation and subsequent apoptosis. Ion channel activity has also been
CC       suggested, however, formation of cation-selective channel has been
CC       reconstituted ex-vivo in lipid bilayers. It is thus unsure that this
CC       activity plays a role in vivo. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- ACTIVITY REGULATION: Ion channel activity is inhibited by hexamethylene
CC       amiloride in vitro. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- SUBUNIT: Forms pentamers or hexamers. Interacts with host CD4 and BRTC;
CC       these interactions induce proteasomal degradation of CD4. Interacts
CC       with host BST2; this interaction leads to the degradation of host BST2.
CC       Interacts with host FBXW11. Interacts with host AP1M1; this interaction
CC       plays a role in the mistrafficking and subsequent degradation of host
CC       BST2. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000255|HAMAP-Rule:MF_04082};
CC       Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- DOMAIN: The N-terminal and transmembrane domains are required for
CC       proper virion budding, whereas the cytoplasmic domain is required for
CC       CD4 degradation. The cytoplasmic domain is composed of 2 amphipathic
CC       alpha helix. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- PTM: Phosphorylated by host CK2. This phosphorylation is necessary for
CC       interaction with human BTRC and degradation of CD4. {ECO:0000255|HAMAP-
CC       Rule:MF_04082}.
CC   -!- MISCELLANEOUS: HIV-1 lineages are divided in three main groups, M (for
CC       Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast
CC       majority of strains found worldwide belong to the group M. Group O
CC       seems to be endemic to and largely confined to Cameroon and neighboring
CC       countries in West Central Africa, where these viruses represent a small
CC       minority of HIV-1 strains. The group N is represented by a limited
CC       number of isolates from Cameroonian persons. The group M is further
CC       subdivided in 9 clades or subtypes (A to D, F to H, J and K).
CC       {ECO:0000255|HAMAP-Rule:MF_04082}.
CC   -!- SIMILARITY: Belongs to the HIV-1 VPU protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04082}.
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DR   EMBL; U46016; AAB36506.1; -; Genomic_DNA.
DR   Proteomes; UP000007694; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0005261; F:cation channel activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042609; F:CD4 receptor binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032801; P:receptor catabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0039587; P:suppression by virus of host tetherin activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-UniRule.
DR   GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.195.10; -; 1.
DR   HAMAP; MF_04082; HIV_VPU; 1.
DR   InterPro; IPR008187; Vpu.
DR   InterPro; IPR009032; Vpu_cyt_dom_sf.
DR   Pfam; PF00558; Vpu; 1.
DR   SUPFAM; SSF57647; SSF57647; 1.
PE   3: Inferred from homology;
KW   AIDS; Apoptosis; Host membrane; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host tetherin by virus; Ion channel; Ion transport; Membrane;
KW   Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport; Viral immunoevasion.
FT   CHAIN           1..86
FT                   /note="Protein Vpu"
FT                   /id="PRO_0000244326"
FT   TOPO_DOM        1..12
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT   TOPO_DOM        34..86
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT   REGION          52..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         58
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT   MOD_RES         62
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
SQ   SEQUENCE   86 AA;  9994 MW;  B611853F6EF6B92E CRC64;
     MVDLLAKVDY RIVIVAFIVA LIIAIVVWTI AYIEYRKLLR QRRIDRLIKR TRERAEDSGN
     ESDGDTEELS TMVDMGNLRL LDVNDL
 
 
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