VPU_HV1LW
ID VPU_HV1LW Reviewed; 81 AA.
AC Q70625;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 02-JUN-2021, entry version 107.
DE RecName: Full=Protein Vpu {ECO:0000255|HAMAP-Rule:MF_04082};
DE AltName: Full=U ORF protein {ECO:0000255|HAMAP-Rule:MF_04082};
DE AltName: Full=Viral protein U {ECO:0000255|HAMAP-Rule:MF_04082};
GN Name=vpu {ECO:0000255|HAMAP-Rule:MF_04082};
OS Human immunodeficiency virus type 1 group M subtype B (isolate LW123)
OS (HIV-1).
OC Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX NCBI_TaxID=82834;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7826699; DOI=10.1089/aid.1994.10.1143;
RA Reitz M.S. Jr., Hall L., Robert-Guroff M., Lautenberger J.A., Hahn B.M.,
RA Shaw G.M., Kong L.I., Weiss S.H., Waters D., Gallo R.C., Blattner W.;
RT "Viral variability and serum antibody response in a laboratory worker
RT infected with HIV type 1 (HTLV type IIIB).";
RL AIDS Res. Hum. Retroviruses 10:1143-1155(1994).
RN [2]
RP STRUCTURE BY NMR OF 1-30.
RX PubMed=14529626; DOI=10.1016/j.jmb.2003.08.048;
RA Park S.H., Mrse A.A., Nevzorov A.A., Mesleh M.F., Oblatt-Montal M.,
RA Montal M., Opella S.J.;
RT "Three-dimensional structure of the channel-forming trans-membrane domain
RT of virus protein 'u' (Vpu) from HIV-1.";
RL J. Mol. Biol. 333:409-424(2003).
CC -!- FUNCTION: Enhances virion budding by targeting host CD4 and
CC Tetherin/BST2 to proteasome degradation. Degradation of CD4 prevents
CC any unwanted premature interactions between viral Env and its host
CC receptor CD4 in the endoplasmic reticulum. Degradation of
CC antiretroviral protein Tetherin/BST2 is important for virion budding,
CC as BST2 tethers new viral particles to the host cell membrane.
CC Mechanistically, Vpu bridges either CD4 or BST2 to BTRC, a substrate
CC recognition subunit of the Skp1/Cullin/F-box protein E3 ubiquitin
CC ligase, induces their ubiquitination and subsequent proteasomal
CC degradation. The alteration of the E3 ligase specificity by Vpu seems
CC to promote the degradation of host IKBKB, leading to NF-kappa-B down-
CC regulation and subsequent apoptosis. Ion channel activity has also been
CC suggested, however, formation of cation-selective channel has been
CC reconstituted ex-vivo in lipid bilayers. It is thus unsure that this
CC activity plays a role in vivo. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- ACTIVITY REGULATION: Ion channel activity is inhibited by hexamethylene
CC amiloride in vitro. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- SUBUNIT: Forms pentamers or hexamers. Interacts with host CD4 and BRTC;
CC these interactions induce proteasomal degradation of CD4. Interacts
CC with host BST2; this interaction leads to the degradation of host BST2.
CC Interacts with host FBXW11. Interacts with host AP1M1; this interaction
CC plays a role in the mistrafficking and subsequent degradation of host
CC BST2. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000255|HAMAP-Rule:MF_04082};
CC Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- DOMAIN: The N-terminal and transmembrane domains are required for
CC proper virion budding, whereas the cytoplasmic domain is required for
CC CD4 degradation. The cytoplasmic domain is composed of 2 amphipathic
CC alpha helix. {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- PTM: Phosphorylated by host CK2. This phosphorylation is necessary for
CC interaction with human BTRC and degradation of CD4. {ECO:0000255|HAMAP-
CC Rule:MF_04082}.
CC -!- MISCELLANEOUS: HIV-1 lineages are divided in three main groups, M (for
CC Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast
CC majority of strains found worldwide belong to the group M. Group O
CC seems to be endemic to and largely confined to Cameroon and neighboring
CC countries in West Central Africa, where these viruses represent a small
CC minority of HIV-1 strains. The group N is represented by a limited
CC number of isolates from Cameroonian persons. The group M is further
CC subdivided in 9 clades or subtypes (A to D, F to H, J and K).
CC {ECO:0000255|HAMAP-Rule:MF_04082}.
CC -!- SIMILARITY: Belongs to the HIV-1 VPU protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04082}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U12055; AAA76689.1; -; Genomic_RNA.
DR PDB; 1PI7; NMR; -; A=1-28.
DR PDB; 1PI8; NMR; -; A=1-28.
DR PDB; 1PJE; NMR; -; A=1-28.
DR PDB; 2GOF; NMR; -; A=2-28.
DR PDB; 2GOH; NMR; -; A=2-28.
DR PDBsum; 1PI7; -.
DR PDBsum; 1PI8; -.
DR PDBsum; 1PJE; -.
DR PDBsum; 2GOF; -.
DR PDBsum; 2GOH; -.
DR SMR; Q70625; -.
DR EvolutionaryTrace; Q70625; -.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005261; F:cation channel activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042609; F:CD4 receptor binding; IEA:UniProtKB-UniRule.
DR GO; GO:0032801; P:receptor catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0039587; P:suppression by virus of host tetherin activity; IEA:UniProtKB-UniRule.
DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-UniRule.
DR GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.195.10; -; 1.
DR HAMAP; MF_04082; HIV_VPU; 1.
DR InterPro; IPR008187; Vpu.
DR InterPro; IPR009032; Vpu_cyt_dom_sf.
DR Pfam; PF00558; Vpu; 1.
DR SUPFAM; SSF57647; SSF57647; 1.
PE 1: Evidence at protein level;
KW 3D-structure; AIDS; Apoptosis; Host membrane; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host interferon signaling pathway by virus;
KW Inhibition of host tetherin by virus; Ion channel; Ion transport; Membrane;
KW Phosphoprotein; Transmembrane; Transmembrane helix; Transport;
KW Viral immunoevasion.
FT CHAIN 1..81
FT /note="Protein Vpu"
FT /id="PRO_0000085410"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT TOPO_DOM 28..81
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT MOD_RES 52
FT /note="Phosphoserine; by host CK2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT MOD_RES 56
FT /note="Phosphoserine; by host CK2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04082"
FT HELIX 8..24
FT /evidence="ECO:0007829|PDB:1PI7"
SQ SEQUENCE 81 AA; 9146 MW; AB74B4A7CFEE4B51 CRC64;
MQPIQIAIVA LVVAIIIAIV VWSIVIIEYR KILRQRKIDR LIDRLIERAE DSGNESEGEI
SALAEMGVEM GHHAPWDVDD L
