ID   VPX_SIVM1               Reviewed;         112 AA.
AC   P05917;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   29-SEP-2021, entry version 71.
DE   RecName: Full=Protein Vpx;
DE   AltName: Full=Viral protein X;
DE   AltName: Full=X ORF protein;
GN   Name=vpx;
OS   Simian immunodeficiency virus (isolate Mm142-83) (SIV-mac) (Simian
OS   immunodeficiency virus rhesus monkey).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX   NCBI_TaxID=11733;
OH   NCBI_TaxID=9527; Cercopithecidae (Old World monkeys).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3649576; DOI=10.1038/328543a0;
RA   Chakrabarti L., Guyader M., Alizon M., Daniel M.D., Desrosiers R.C.,
RA   Tiollais P., Sonigo P.;
RT   "Sequence of simian immunodeficiency virus from macaque and its
RT   relationship to other human and simian retroviruses.";
RL   Nature 328:543-547(1987).
CC   -!- FUNCTION: Plays a role in nuclear translocation of the viral pre-
CC       integration complex (PIC), thus is required for the virus to infect
CC       non-dividing cells. Targets specific host proteins for degradation by
CC       the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3
CC       ligase complex through direct interaction with host VPRPB/DCAF-1. This
CC       change in the E3 ligase substrate specificity results in the
CC       degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral
CC       replication in host myeloid cells by preventing SAMHD1-mediated
CC       hydrolysis of intracellular dNTPs necessary for reverse transcription
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the P6 region of unprocessed GAG. Interacts
CC       with host VPRBP/DCAF1, leading to change substrate specificity of the
CC       CUL4A-DDB1 E3 ligase complex (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host nucleus. Note=Nuclear just after
CC       virion uncoating, or if expressed in the absence of unprocessed GAG.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: This is a macaque isolate.
CC   -!- SIMILARITY: Belongs to the lentivirus VPX protein family.
CC       {ECO:0000305}.
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DR   EMBL; Y00277; CAA68382.1; -; Genomic_DNA.
DR   SMR; P05917; -.
DR   Proteomes; UP000007220; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0019058; P:viral life cycle; IEA:InterPro.
DR   InterPro; IPR000012; RetroV_VpR/X.
DR   Pfam; PF00522; VPR; 1.
PE   3: Inferred from homology;
KW   Host nucleus; Host-virus interaction;
KW   Inhibition of host innate immune response by virus; Viral immunoevasion;
KW   Virion.
FT   CHAIN           1..112
FT                   /note="Protein Vpx"
FT                   /id="PRO_0000085403"
FT   MOTIF           65..72
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   112 AA;  12906 MW;  46F564F45AFFD960 CRC64;
     MSDPRERIPP GNSGEETIGE AFEWLNRTVE EINREAVNHL PRELIFQVWQ RSWEYWHDEQ
     GMSQSYTKYR YLCLIQKALF MHCKKGCRCL GEGHGAGGWR PGPPPPPPPG LA