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VPY_MEDTR
ID   VPY_MEDTR               Reviewed;         541 AA.
AC   D3J162;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Protein VAPYRIN {ECO:0000303|PubMed:19912567};
DE            Short=MtVpy {ECO:0000303|PubMed:19912567};
DE   AltName: Full=Protein HERMES {ECO:0000303|PubMed:21223389};
GN   Name=VPY {ECO:0000303|PubMed:19912567};
GN   Synonyms=FNB4 {ECO:0000303|PubMed:21223389},
GN   HMS {ECO:0000303|PubMed:21223389};
GN   OrderedLocusNames=MTR_6g027840 {ECO:0000312|EMBL:KEH25576.1};
GN   ORFNames=MtrunA17_Chr6g0461061 {ECO:0000312|EMBL:RHN50766.1};
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP   DEVELOPMENTAL STAGE, INDUCTION BY GLOMUS VERSIFORME AND RHIZOBIA BACTERIA,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=19912567; DOI=10.1111/j.1365-313x.2009.04072.x;
RA   Pumplin N., Mondo S.J., Topp S., Starker C.G., Gantt J.S., Harrison M.J.;
RT   "Medicago truncatula Vapyrin is a novel protein required for arbuscular
RT   mycorrhizal symbiosis.";
RL   Plant J. 61:482-494(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP   INDUCTION BY SINORHIZOBIUM MELILOTI NOD FACTORS, TISSUE SPECIFICITY,
RP   INDUCTION BY MYCORRHIZAL FUNGI AND NITROGEN-FIXING RHIZOBIAL BACTERIA, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=21223389; DOI=10.1111/j.1365-313x.2010.04415.x;
RA   Murray J.D., Muni R.R., Torres-Jerez I., Tang Y., Allen S., Andriankaja M.,
RA   Li G., Laxmi A., Cheng X., Wen J., Vaughan D., Schultze M., Sun J.,
RA   Charpentier M., Oldroyd G., Tadege M., Ratet P., Mysore K.S., Chen R.,
RA   Udvardi M.K.;
RT   "Vapyrin, a gene essential for intracellular progression of arbuscular
RT   mycorrhizal symbiosis, is also essential for infection by rhizobia in the
RT   nodule symbiosis of Medicago truncatula.";
RL   Plant J. 65:244-252(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=22089132; DOI=10.1038/nature10625;
RA   Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA   Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA   Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA   De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA   Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA   Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA   Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA   Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA   Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA   Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA   Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA   Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA   Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA   O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA   Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA   Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA   Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA   Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA   White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA   Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA   Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT   "The Medicago genome provides insight into the evolution of rhizobial
RT   symbioses.";
RL   Nature 480:520-524(2011).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA   Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA   Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA   Schwartz D.C., Town C.D.;
RT   "An improved genome release (version Mt4.0) for the model legume Medicago
RT   truncatula.";
RL   BMC Genomics 15:312-312(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA   Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA   Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA   Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA   Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA   Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA   Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT   "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL   Nat. Plants 4:1017-1025(2018).
RN   [6]
RP   INTERACTION WITH EX70I, AND SUBCELLULAR LOCATION.
RX   PubMed=26234213; DOI=10.1016/j.cub.2015.06.075;
RA   Zhang X., Pumplin N., Ivanov S., Harrison M.J.;
RT   "EXO70I is required for development of a sub-domain of the periarbuscular
RT   membrane during arbuscular mycorrhizal symbiosis.";
RL   Curr. Biol. 25:2189-2195(2015).
RN   [7]
RP   INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX   PubMed=26511916; DOI=10.1104/pp.15.01155;
RA   Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT   "Hyphal branching during arbuscule development requires reduced arbuscular
RT   mycorrhiza1.";
RL   Plant Physiol. 169:2774-2788(2015).
CC   -!- FUNCTION: Required for arbuscular mycorrhizal (AM) symbiosis with AM
CC       fungi (e.g. Glomus versiforme and Gigaspora gigantea) both during
CC       fungal passage across root epidermis and for arbuscule formation in
CC       cortical cells; this symbiosis promotes phosphorus (P) and copper (Cu)
CC       uptake (PubMed:19912567, PubMed:21223389). Essential for infection by
CC       symbiotic nitrogen-fixing rhizobial bacteria (e.g. Sinorhizobium
CC       meliloti) leading to the formation of root nodules (PubMed:21223389).
CC       {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389}.
CC   -!- SUBUNIT: Interacts with EX70I at the periarbuscular membrane (PAM)
CC       around the arbuscule hyphal tips. {ECO:0000269|PubMed:26234213}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19912567,
CC       ECO:0000269|PubMed:21223389}. Nucleus {ECO:0000269|PubMed:19912567,
CC       ECO:0000269|PubMed:21223389}. Cell membrane
CC       {ECO:0000269|PubMed:26234213}; Peripheral membrane protein
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=In cells containing
CC       arbuscular mycorrhizal (AM) fungal hyphae and arbuscules, accumulates
CC       in small puncta that move through the cytoplasm, likely mobile
CC       spherical structures that are associated with the tonoplast referred to
CC       as 'tonospheres' (PubMed:19912567). Present in cytoplasmic strands
CC       below hyphopodia (PubMed:19912567). Observed associated with EX70I in
CC       zones adjacent to the periarbuscular membrane (PAM) around the
CC       arbuscule hyphal tips (PubMed:26234213). Occasionally observed in the
CC       nucleus of cells containing fungal hyphae (PubMed:19912567).
CC       {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:26234213}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:19912567,
CC       ECO:0000269|PubMed:21223389}.
CC   -!- DEVELOPMENTAL STAGE: First observed in root vascular tissues and root
CC       caps (PubMed:19912567). During arbuscular mycorrhizal (AM) symbiosis
CC       with (AM) fungi (e.g. Glomus versiforme), accumulates transiently in
CC       the root epidermis and outer cortical cells coincident with hyphal
CC       penetration (hyphopodia), and later present in cortex cells during
CC       arbuscule formation (PubMed:19912567). {ECO:0000269|PubMed:19912567}.
CC   -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC       colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus
CC       versiforme and Gigaspora gigantea) (PubMed:19912567, PubMed:21223389,
CC       PubMed:26511916). Induced in roots (including root hairs) by rhizobial
CC       bacteria (e.g. Sinorhizobium meliloti) synthesized Nod factors in a
CC       NFP, DMI1 and DMI3-dependent manner (PubMed:21223389). Also observed in
CC       root nodules that arise from symbiotic associations with nitrogen-
CC       fixing Rhizobia (PubMed:19912567, PubMed:21223389).
CC       {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389,
CC       ECO:0000269|PubMed:26511916}.
CC   -!- DISRUPTION PHENOTYPE: Impaired arbuscular mycorrhiza (AM) fungi (e.g.
CC       Glomus versiforme and Gigaspora gigantea) passage across root epidermis
CC       and abolished arbuscule formation in root cortex during AM symbiosis,
CC       thus leading to aborted hyphopodia and no arbuscules (PubMed:19912567,
CC       PubMed:21223389). Reduced accumulation of PT4 in AM roots
CC       (PubMed:19912567). Abnormal nitrogen-fixing rhizobial bacteria (e.g.
CC       Sinorhizobium meliloti) infection threads and fewer small white root
CC       nodules, but normal calcium spiking in root hairs in response to Nod
CC       factors (PubMed:21223389). {ECO:0000269|PubMed:19912567,
CC       ECO:0000269|PubMed:21223389}.
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DR   EMBL; GQ423209; ADC33495.1; -; Genomic_DNA.
DR   EMBL; FJ795648; ACX94227.1; -; Genomic_DNA.
DR   EMBL; CM001222; KEH25576.1; -; Genomic_DNA.
DR   EMBL; PSQE01000006; RHN50766.1; -; Genomic_DNA.
DR   RefSeq; XP_013451548.1; XM_013596094.1.
DR   AlphaFoldDB; D3J162; -.
DR   SMR; D3J162; -.
DR   EnsemblPlants; KEH25576; KEH25576; MTR_6g027840.
DR   GeneID; 25495843; -.
DR   Gramene; KEH25576; KEH25576; MTR_6g027840.
DR   KEGG; mtr:MTR_6g027840; -.
DR   HOGENOM; CLU_000134_53_1_1; -.
DR   OrthoDB; 460473at2759; -.
DR   Proteomes; UP000002051; Chromosome 6.
DR   Proteomes; UP000265566; Chromosome 6.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0085042; C:periarbuscular membrane; IDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR   GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR   GO; GO:0002237; P:response to molecule of bacterial origin; IEP:UniProtKB.
DR   GO; GO:0009609; P:response to symbiotic bacterium; IMP:UniProtKB.
DR   GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR   Gene3D; 1.25.40.20; -; 4.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR000535; MSP_dom.
DR   InterPro; IPR008962; PapD-like_sf.
DR   Pfam; PF12796; Ank_2; 4.
DR   Pfam; PF00635; Motile_Sperm; 1.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 8.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   SUPFAM; SSF49354; SSF49354; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 7.
DR   PROSITE; PS50202; MSP; 1.
PE   1: Evidence at protein level;
KW   ANK repeat; Cell membrane; Cytoplasm; Membrane; Nucleus;
KW   Reference proteome; Repeat.
FT   CHAIN           1..541
FT                   /note="Protein VAPYRIN"
FT                   /id="PRO_0000450029"
FT   DOMAIN          4..138
FT                   /note="MSP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT   REPEAT          176..205
FT                   /note="ANK 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          209..238
FT                   /note="ANK 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          242..271
FT                   /note="ANK 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          275..304
FT                   /note="ANK 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          309..338
FT                   /note="ANK 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          342..372
FT                   /note="ANK 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          374..392
FT                   /note="ANK 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          396..425
FT                   /note="ANK 8"
FT                   /evidence="ECO:0000255"
FT   REPEAT          429..458
FT                   /note="ANK 9"
FT                   /evidence="ECO:0000255"
FT   REPEAT          462..491
FT                   /note="ANK 10"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   541 AA;  59134 MW;  8E66558DE0998BEA CRC64;
     MDRLIKLDPS NIVLIRVEEG QKCLGKITLN NVMYTMPVAF RIQPLIKTRY TIKPQSGIIS
     PLASLVIEIT YHPPQQQGSN NLPHSFPFSD DSFLLHSVLA PGAAIKEPSS MFDSVPSDWF
     TTKKKQVFID SAIKVMFVGS QILTQLVEDG NSMDDIREVL EKSDPLWESV NSKDSQGQTL
     LHLAISKTRP DLVQLILEFK PDIEAINSVG STPLEAASSS GESLIVELLL AHKANTEGSE
     SSVFRPIHHA SREGHMEILR LLLLKGARVD SLTKDGNTSL HLAVEEKRRD CARLLLANGA
     RTDVRNMREG DTPLHIAAAN GDENMVKLLL HKGATKYVRN KLGKTAFDVA AENGHSRLFD
     ALRLGDNLCA AARKGEVRTI QKVLESGGVI NGRDQNGWTS LHRAAFKGRM DAVRFLVEKG
     IDLDAKDEDG YTALHCAAES GHADVTEFLV KKGADVEART NKGVSALQIV ESLNYVGITR
     ILVNGGASRE GLGEKPPSAP SKIPFGRKVE SGSVMTMKKK MSSRTRALRG SFDHSMPLAV
     L
 
 
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