VQ31_ARATH
ID VQ31_ARATH Reviewed; 173 AA.
AC Q9FNP0; A0MFE8; Q8LE89;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=VQ motif-containing protein 31 {ECO:0000303|PubMed:22535423};
DE Short=AtVQ31 {ECO:0000303|PubMed:22535423};
DE AltName: Full=MPK3/6-targeted VQ-motif-containing protein 6 {ECO:0000303|PubMed:24750137};
GN Name=VQ31 {ECO:0000303|PubMed:22535423};
GN Synonyms=MVQ6 {ECO:0000303|PubMed:24750137};
GN OrderedLocusNames=At5g08480 {ECO:0000312|Araport:AT5G08480};
GN ORFNames=MAH20.4 {ECO:0000312|EMBL:BAB09997.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22535423; DOI=10.1104/pp.112.196816;
RA Cheng Y., Zhou Y., Yang Y., Chi Y.J., Zhou J., Chen J.Y., Wang F., Fan B.,
RA Shi K., Zhou Y.H., Yu J.Q., Chen Z.;
RT "Structural and functional analysis of VQ motif-containing proteins in
RT Arabidopsis as interacting proteins of WRKY transcription factors.";
RL Plant Physiol. 159:810-825(2012).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND PHOSPHORYLATION AT THR-46 AND
RP SER-146.
RX PubMed=24750137; DOI=10.1111/nph.12817;
RA Pecher P., Eschen-Lippold L., Herklotz S., Kuhle K., Naumann K., Bethke G.,
RA Uhrig J., Weyhe M., Scheel D., Lee J.;
RT "The Arabidopsis thaliana mitogen-activated protein kinases MPK3 and MPK6
RT target a subclass of 'VQ-motif'-containing proteins to regulate immune
RT responses.";
RL New Phytol. 203:592-606(2014).
CC -!- FUNCTION: May modulate WRKY transcription factor activities.
CC {ECO:0000250|UniProtKB:Q9M9F0}.
CC -!- INTERACTION:
CC Q9FNP0; Q9FFS3: WRKY24; NbExp=3; IntAct=EBI-25517821, EBI-4431481;
CC Q9FNP0; Q8GY11: WRKY43; NbExp=3; IntAct=EBI-25517821, EBI-1235953;
CC Q9FNP0; Q8VWQ4: WRKY56; NbExp=3; IntAct=EBI-25517821, EBI-25517688;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9M9F0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FNP0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FNP0-2; Sequence=VSP_057494;
CC -!- PTM: Phosphorylated on serine and threonine residues by MPK6.
CC {ECO:0000269|PubMed:24750137}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28690.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB006697; BAB09997.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91308.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91309.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68666.1; -; Genomic_DNA.
DR EMBL; DQ446932; ABE66143.1; -; mRNA.
DR EMBL; DQ446933; ABE66144.1; -; mRNA.
DR EMBL; DQ653274; ABK28690.1; ALT_SEQ; mRNA.
DR EMBL; BT026100; ABG48456.1; -; mRNA.
DR EMBL; AY085562; AAM62784.1; -; mRNA.
DR RefSeq; NP_001318511.1; NM_001343006.1. [Q9FNP0-2]
DR RefSeq; NP_001330395.1; NM_001343007.1. [Q9FNP0-1]
DR RefSeq; NP_850793.1; NM_180462.2. [Q9FNP0-1]
DR AlphaFoldDB; Q9FNP0; -.
DR IntAct; Q9FNP0; 3.
DR STRING; 3702.AT5G08480.2; -.
DR iPTMnet; Q9FNP0; -.
DR PaxDb; Q9FNP0; -.
DR PRIDE; Q9FNP0; -.
DR ProteomicsDB; 242752; -. [Q9FNP0-1]
DR EnsemblPlants; AT5G08480.1; AT5G08480.1; AT5G08480. [Q9FNP0-2]
DR EnsemblPlants; AT5G08480.2; AT5G08480.2; AT5G08480. [Q9FNP0-1]
DR EnsemblPlants; AT5G08480.3; AT5G08480.3; AT5G08480. [Q9FNP0-1]
DR GeneID; 830747; -.
DR Gramene; AT5G08480.1; AT5G08480.1; AT5G08480. [Q9FNP0-2]
DR Gramene; AT5G08480.2; AT5G08480.2; AT5G08480. [Q9FNP0-1]
DR Gramene; AT5G08480.3; AT5G08480.3; AT5G08480. [Q9FNP0-1]
DR KEGG; ath:AT5G08480; -.
DR Araport; AT5G08480; -.
DR TAIR; locus:2159567; AT5G08480.
DR eggNOG; ENOG502S1DP; Eukaryota.
DR InParanoid; Q9FNP0; -.
DR OMA; HERRQYM; -.
DR PhylomeDB; Q9FNP0; -.
DR PRO; PR:Q9FNP0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNP0; differential.
DR Genevisible; Q9FNP0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR008889; VQ.
DR InterPro; IPR039829; VQ31.
DR InterPro; IPR039611; VQ_4/11/13/19/31/33.
DR PANTHER; PTHR33402; PTHR33402; 1.
DR PANTHER; PTHR33402:SF30; PTHR33402:SF30; 1.
DR Pfam; PF05678; VQ; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..173
FT /note="VQ motif-containing protein 31"
FT /id="PRO_0000432321"
FT REGION 76..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 27..36
FT /note="VQ"
FT /evidence="ECO:0000305"
FT COMPBIAS 85..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 46
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:24750137"
FT MOD_RES 92
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LDZ1"
FT MOD_RES 103
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LDZ1"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:24750137"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9FHZ3"
FT MOD_RES 165
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O23660"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LDZ1"
FT MOD_RES 170
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O23660"
FT VAR_SEQ 88..118
FT /note="Missing (in isoform 2)"
FT /id="VSP_057494"
SQ SEQUENCE 173 AA; 18899 MW; A2DF7C89A72F532E CRC64;
MNSKGSQNVA TTCKPVTTFV QTDTNTFREI VQRLTGPTEN NAAAATPEAT VIKTAIQKRP
TSKLHERRQC MRPKLEIVKP PLSFKPTGTT PSSKSGNTNL LTSPVGTPSS LFSNLSLIEG
EPDSCTTNIE EEEKAIKERR FYLHPSPRSK PGYTEPELLT LFPLTSPNSS GKP
