ID   VQSM_PSEAE              Reviewed;         325 AA.
AC   Q9I1P2;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=HTH-type transcriptional regulator VqsM;
DE   AltName: Full=Virulence and quorum sensing modulator protein;
GN   Name=vqsM; OrderedLocusNames=PA2227;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=16194239; DOI=10.1111/j.1365-2958.2005.04851.x;
RA   Dong Y.-H., Zhang X.-F., Xu J.-L., Tan A.-T., Zhang L.-H.;
RT   "VqsM, a novel AraC-type global regulator of quorum-sensing signalling and
RT   virulence in Pseudomonas aeruginosa.";
RL   Mol. Microbiol. 58:552-564(2005).
CC   -!- FUNCTION: Transcriptional regulator involved in both the repression (at
CC       least 99 genes, such as mexR and algU) and in the activation (at least
CC       203 genes, such as mvfR, rsaL, vqsR and rpoS) of regulatory or putative
CC       regulatory proteins which are implicated in quorum sensing, virulence
CC       and multidrug resistance. {ECO:0000269|PubMed:16194239}.
CC   -!- MISCELLANEOUS: Mutation in vqsM resulted in loss of proteolytic and
CC       elastase activity, significantly decreased expression of lasI and rhlI,
CC       which are responsible for acyl homoserine lactone (acyl-HSL)
CC       biosynthesis, much reduced swimming and swarming capacity as well as
CC       decreased capacity of killing C.elegans.
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DR   EMBL; AE004091; AAG05615.1; -; Genomic_DNA.
DR   PIR; D83366; D83366.
DR   RefSeq; NP_250917.1; NC_002516.2.
DR   RefSeq; WP_003113714.1; NZ_QZGE01000014.1.
DR   AlphaFoldDB; Q9I1P2; -.
DR   SMR; Q9I1P2; -.
DR   STRING; 287.DR97_6324; -.
DR   PaxDb; Q9I1P2; -.
DR   DNASU; 882297; -.
DR   EnsemblBacteria; AAG05615; AAG05615; PA2227.
DR   GeneID; 882297; -.
DR   KEGG; pae:PA2227; -.
DR   PATRIC; fig|208964.12.peg.2327; -.
DR   PseudoCAP; PA2227; -.
DR   HOGENOM; CLU_047522_3_1_6; -.
DR   PhylomeDB; Q9I1P2; -.
DR   BioCyc; PAER208964:G1FZ6-2267-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   CollecTF; EXPREG_00001670; -.
DR   GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEP:CollecTF.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   InterPro; IPR032687; AraC-type_N.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   Pfam; PF12625; Arabinose_bd; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Quorum sensing; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..325
FT                   /note="HTH-type transcriptional regulator VqsM"
FT                   /id="PRO_0000252690"
FT   DOMAIN          226..323
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        243..264
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        290..313
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   325 AA;  37004 MW;  665FECBDC24D17DE CRC64;
     MVTEHIFDFE WRPLQNYLRD RDIAIPPFAA EQESRSDALY NWIVSKGYSG KEGLNLGTYY
     HISDYGVIGL ALLCAENVGD ILKVIRAYVK LFNRDIANVG VKPRENYEVE IHISVNFKPE
     WNDASRQFHV NVIASATYKL IMDLLGNDFP ISALTVPVHG ADKTIYEGFF SLPVKHEGND
     IIFSFPAHQL NRTLATANPA VFQSALTMAG ESFNALLEVE MGGLRQRIEL FLDSIPDQYP
     SLVTTAKYLR MNERTVRRRL ADEGCTYRQI VDKARKERAI ALLLNSSIPV DRISDILGYS
     ETASFRHAFR RWTGQSTTEF RNSFH