ID   VR2A_BPT4               Reviewed;         725 AA.
AC   P03690; O36165;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Protein rIIA;
GN   Name=rIIA;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2379817; DOI=10.1093/genetics/125.2.237;
RA   Daegelen P., Brody E.;
RT   "The rIIA gene of bacteriophage T4. I. Its DNA sequence and discovery of a
RT   new open reading frame between genes 60 and rIIA.";
RL   Genetics 125:237-248(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-725.
RX   PubMed=7916730; DOI=10.1093/genetics/135.2.255;
RA   Kumagai M., Yamashita T., Honda M., Ikeda H.;
RT   "Effects of uvsX, uvsY and DNA topoisomerase on the formation of tandem
RT   duplications of the rII gene in bacteriophage T4.";
RL   Genetics 135:255-264(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 476-725.
RX   PubMed=6748077; DOI=10.1016/0022-2836(84)90422-4;
RA   Sugino A., Drake J.W.;
RT   "Modulation of mutation rates in bacteriophage T4 by a base-pair change a
RT   dozen nucleotides removed.";
RL   J. Mol. Biol. 176:239-249(1984).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 587-725.
RX   PubMed=6273585; DOI=10.1016/0022-2836(81)90477-0;
RA   Pribnow D., Sigurdson D.C., Gold L., Singer B.S., Napoli C., Brosius J.,
RA   Dull T.J., Noller H.F.;
RT   "rII cistrons of bacteriophage T4. DNA sequence around the intercistronic
RT   divide and positions of genetic landmarks.";
RL   J. Mol. Biol. 149:337-376(1981).
RN   [6]
RP   FUNCTION.
RX   PubMed=6757252; DOI=10.1016/s0021-9258(18)33397-0;
RA   Chiu C.S., Cook K.S., Greenberg G.R.;
RT   "Characteristics of a bacteriophage T4-induced complex synthesizing
RT   deoxyribonucleotides.";
RL   J. Biol. Chem. 257:15087-15097(1982).
RN   [7]
RP   INDUCTION.
RX   PubMed=2379818; DOI=10.1093/genetics/125.2.249;
RA   Daegelen P., Brody E.;
RT   "The rIIA gene of bacteriophage T4. II. Regulation of its messenger RNA
RT   synthesis.";
RL   Genetics 125:249-260(1990).
RN   [8]
RP   FUNCTION.
RX   PubMed=9560373; DOI=10.1093/genetics/148.4.1539;
RA   Paddison P., Abedon S.T., Dressman H.K., Gailbreath K., Tracy J.,
RA   Mosser E., Neitzel J., Guttman B., Kutter E.;
RT   "The roles of the bacteriophage T4 r genes in lysis inhibition and fine-
RT   structure genetics: a new perspective.";
RL   Genetics 148:1539-1550(1998).
CC   -!- FUNCTION: May play a role in viral replication since found in complexes
CC       carrying several of the enzymes synthesizing deoxyribonucleotides and
CC       associated with DNA and host membrane. May be required for maintaining
CC       membrane integrity during the process. {ECO:0000269|PubMed:6757252,
CC       ECO:0000269|PubMed:9560373}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane. Note=On the inner
CC       membrane of the host shortly after infection.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       rIIA RNA is translationally repressed by the regA protein.
CC       {ECO:0000269|PubMed:2379818}.
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DR   EMBL; X52686; CAA36911.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42647.1; -; Genomic_DNA.
DR   EMBL; D16359; BAA23634.1; -; Genomic_DNA.
DR   PIR; JU0400; Z2BPA4.
DR   RefSeq; NP_049616.1; NC_000866.4.
DR   GeneID; 1258593; -.
DR   KEGG; vg:1258593; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   SUPFAM; SSF55874; SSF55874; 1.
PE   2: Evidence at transcript level;
KW   DNA replication; DNA-binding; Early protein; Host cell inner membrane;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Repeat.
FT   CHAIN           1..725
FT                   /note="Protein rIIA"
FT                   /id="PRO_0000165070"
FT   REPEAT          101..179
FT                   /note="Approximate"
FT   REPEAT          481..559
FT                   /note="Approximate"
FT   DNA_BIND        544..570
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        616
FT                   /note="D -> H (in Ref. 4)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   725 AA;  82893 MW;  2290FE341BB45416 CRC64;
     MIITTEKETI LGNGSKSKAF SITASPKVFK ILSSDLYTNK IRAVVRELIT NMIDAHALNG
     NPEKFIIQVP GRLDPRFVCR DFGPGMSDFD IQGDDNSPGL YNSYFSSSKA ESNDFIGGFG
     LGSKSPFSYT DTFSITSYHK GEIRGYVAYM DGDGPQIKPT FVKEMGPDDK TGIEIVVPVE
     EKDFRNFAYE VSYIMRPFKD LAIINGLDRE IDYFPDFDDY YGVNPERYWP DRGGLYAIYG
     GIVYPIDGVI RDRNWLSIRN EVNYIKFPMG SLDIAPSREA LSLDDRTRKN IIERVKELSE
     KAFNEDVKRF KESTSPRHTY RELMKMGYSA RDYMISNSVK FTTKNLSYKK MQSMFEPDSK
     LCNAGVVYEV NLDPRLKRIK QSHETSAVAS SYRLFGINTT KINIVIDNIK NRVNIVRGLA
     RALDDSEFNN TLNIHHNERL LFINPEVESQ IDLLPDIMAM FESDEVNIHY LSEIEALVKS
     YIPKVVKSKA PRPKAATAFK FEIKDGRWEK RNYLRLTSEA DEITGYVAYM HRSDIFSMDG
     TTSLCHPSMN ILIRMANLIG INEFYVIRPL LQKKVKELGQ CQCIFEALRD LYVDAFDDVD
     YDKYVGYSSS AKRYIDKIIK YPELDFMMKY FSIDEVSEEY TRLANMVSSL QGVYFNGGKD
     TIGHDIWTVT NLFDVLSNNA SKNSDKMVAE FTKKFRIVSD FIGYRNSLSD DEVSQIAKTM
     KALAA