ID   VRAB_STAAC              Reviewed;         379 AA.
AC   Q5HIA0;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Putative acetyl-CoA C-acetyltransferase VraB;
DE            EC=2.3.1.-;
GN   Name=vraB; OrderedLocusNames=SACOL0622;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000046; AAW37731.1; -; Genomic_DNA.
DR   RefSeq; WP_001070681.1; NC_002951.2.
DR   AlphaFoldDB; Q5HIA0; -.
DR   SMR; Q5HIA0; -.
DR   EnsemblBacteria; AAW37731; AAW37731; SACOL0622.
DR   KEGG; sac:SACOL0622; -.
DR   HOGENOM; CLU_031026_2_1_9; -.
DR   OMA; GHPWGAS; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR   PROSITE; PS00737; THIOLASE_2; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..379
FT                   /note="Putative acetyl-CoA C-acetyltransferase VraB"
FT                   /id="PRO_0000206426"
FT   ACT_SITE        86
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   379 AA;  41022 MW;  477876C11342D392 CRC64;
     MNQAVIVAAK RTAFGKYGGT LKHLEPEQLL KPLFQHFKEK YPEVISKIDD VVLGNVVGNG
     GNIARKALLE AGLKDSIPGV TIDRQCGSGL ESVQYACRMI QAGAGKVYIA GGVESTSRAP
     WKIKRPHSVY ETALPEFYER ASFAPEMSDP SMIQGAENVA KMYDVSRGLQ DEFAYRSHQL
     TAENVKNGNI SQEILPITVK GEIFNTDESL KSHIPKDNFG RFKPVIKGGT VTAANSCMKN
     DGAVLLLIME KDMAYELGFE HGLLFKDGVT VGVDSNFPGI GPVPAISNLL KRNQLTIENI
     EVIEINEAFS AQVVACQQAL NISNTQLNIW GGALASGHPY GASGAQLVTR LFYMFDKETM
     IASMGIGGGL GNAALFTRF