ID   VRAB_STAAM              Reviewed;         379 AA.
AC   Q7A2W9;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Putative acetyl-CoA C-acetyltransferase VraB;
DE            EC=2.3.1.-;
GN   Name=vraB; OrderedLocusNames=SAV0576;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   VANCOMYCIN RESISTANCE.
RX   PubMed=10708580; DOI=10.1006/bbrc.2000.2277;
RA   Kuroda M., Kuwahara-Arai K., Hiramatsu K.;
RT   "Identification of the up- and down-regulated genes in vancomycin-resistant
RT   Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential
RT   hybridization method.";
RL   Biochem. Biophys. Res. Commun. 269:485-490(2000).
CC   -!- MISCELLANEOUS: May contribute to vancomycin resistance.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   EMBL; BA000017; BAB56738.1; -; Genomic_DNA.
DR   RefSeq; WP_001070676.1; NC_002758.2.
DR   AlphaFoldDB; Q7A2W9; -.
DR   SMR; Q7A2W9; -.
DR   PaxDb; Q7A2W9; -.
DR   EnsemblBacteria; BAB56738; BAB56738; SAV0576.
DR   KEGG; sav:SAV0576; -.
DR   HOGENOM; CLU_031026_2_1_9; -.
DR   OMA; GHPWGAS; -.
DR   PhylomeDB; Q7A2W9; -.
DR   BioCyc; SAUR158878:SAV_RS03200-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR   PROSITE; PS00737; THIOLASE_2; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..379
FT                   /note="Putative acetyl-CoA C-acetyltransferase VraB"
FT                   /id="PRO_0000206427"
FT   ACT_SITE        86
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   379 AA;  41094 MW;  B62F36E0A437A771 CRC64;
     MNQAVIVAAK RTAFGKYGGT LKHLEPEQLL KPLFQHFKEK YPEVISKIDD VVLGNVVGNG
     GNIARKALLE AGLKDSIPGV TIDRQCGSGL ESVQYACRMI QAGAGKVYIA GGVESTSRAP
     WKIKRPHSVY ETALPEFYER ASFAPEMSDP SMIQGAENVA KMYDVSRELQ DEFAYRSHQL
     TAENVKNGNI SQEILPITVK GEIFNTDESL KSHIPKDNFG RFKPVIKGGT VTAANSCMKN
     DGAVLLLIME KDMAYELGFE HGLLFKDGVT VGVDSNFPGI GPVPAISNLL KRNQLTIENI
     EVIEINEAFS AQVVACQQAL NISNTQLNIW GGALASGHPY GASGAQLVTR LFYMFDKETM
     IASMGIGGGL GNAALFTRF