ID   VRAB_STAEQ              Reviewed;         382 AA.
AC   Q5HRH3;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Putative acetyl-CoA C-acetyltransferase VraB;
DE            EC=2.3.1.-;
GN   Name=vraB; OrderedLocusNames=SERP0220;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000029; AAW53600.1; -; Genomic_DNA.
DR   RefSeq; WP_002445760.1; NC_002976.3.
DR   AlphaFoldDB; Q5HRH3; -.
DR   SMR; Q5HRH3; -.
DR   STRING; 176279.SERP0220; -.
DR   EnsemblBacteria; AAW53600; AAW53600; SERP0220.
DR   KEGG; ser:SERP0220; -.
DR   eggNOG; COG0183; Bacteria.
DR   HOGENOM; CLU_031026_2_1_9; -.
DR   OMA; GHPWGAS; -.
DR   OrthoDB; 1058688at2; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR   PROSITE; PS00737; THIOLASE_2; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..382
FT                   /note="Putative acetyl-CoA C-acetyltransferase VraB"
FT                   /id="PRO_0000206433"
FT   ACT_SITE        86
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   382 AA;  41803 MW;  9E614E4A29140F5B CRC64;
     MKQPVIIAAK RIAFGKYGGR LKHLEPESLL EPLFNHFTDQ YPKVMSLLDD VILGNTVGNG
     GNLARKSLLE AGLDFKIPGI TIDRQCGSGL EAVIQACRMV QSGAGTIYIA GGVESTSRAP
     WKIKRPQSVY ESEFPQFFER APFAREGEDP SMIEAAENVA KKYHISRNEQ DDFAYRSHQL
     ASKNMNNGNI SQEILPFKVK GECFNQDESI KPQLTLKTLG RLKPLLNEGT VTVGNSCMKN
     DGAVLLIVME ENRARQLGFT EGIKFVNSAT VGVQPQYLGV GPVPAVNQLL ARERLTINDI
     NAVELNEAFS SQVIASQQQL NIPLNKLNCW GGAIATGHPY GASGAALVTR LFYMKHQFRT
     VATMGIGGGI GNAALFERWY GN